Some of the newer hydrophilic interaction liquid chromatography (HILIC) columns are reviewed, and experiments performed to categorize columns for optimized separations are described.
Here is a step-by-step approach to investigate the source of ghost peaks in a gradient LC method.
A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS
Detailed examples of light-scattering techniques are presented as well as how their performance compares to MS and other methods.
The exploration of myxobacterial metabolite profiles by LC–MS screening for the presence of new natural products is described. Extracts from fermentations of Myxococcus strains are analysed by UPLC-coupled ESI-TOF mass spectrometry and the obtained data are processed using principal component analysis (PCA). The generation of molecular formulae from accurate mass measurements facilitates rapid compound identification.
Here's how these new reference standards were characterized.
Nano LC is now widely applied in proteomics, and also is robust enough for application in fields such as pharmaceutical analysis.
This application note describes the method for detection of nanogram levels of explosive constituents in seawater samples (e.g., after detonation of unexploded ordnance devices). The improved chromatographic method was developed using the Agilent 1290 Infinity LC Method Development system.
We explore advancements in silica particles for liquid chromatography, focusing on the shift toward improved monodispersity and its impact on chromatographic parameters, efficiency, backpressure, and sensitivity in high-performance liquid chromatography (HPLC).
An analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described.
A look at the role of system suitability tests (SSTs) during performance qualification (PQ).
A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS
A sensitive and selective liquid chromatography spectrometry mass spectrometry (LC–MS–MS) method to determine clenbuterol-like beta agonist residues in human hair was developed and validated.
Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.
This article details a recent experiment that demonstrates how nonspecific interactions can be prevented by using metal-free or low adsorption flow paths.
A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS
Describing developments in LC–MS and GC–MS that reveal the ageing chemistry of whiskey aged in oak barrels.
This new data-management method is specifically designed to handle the large amounts of data produced in proteomics studies. Experimental methods and data are presented from a recent study conducting proteomics profiling of Schizosaccharomyces pombe to demonstrate the effectiveness of the data management solution. The results demonstrate enhanced data search speeds as well as effective data analysis.
This article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.
How does one decipher and tabulate thousands of chemical formulas and relative abundances without losing the important details?
High-definition screening by gas chromatography–mass spectrometry (GC–MS) is shown to be a viable option for the reliable identification of odorous compounds in pork.
A look at the role of system suitability tests (SSTs) during performance qualification (PQ).
Compounds such as ibuprofen, chlorpheniramine, and allantoin are important in the field of pharmaceuticals and cosmetics. The analysis of acidic and basic compounds is particularly important but it is sometimes difficult to analyze both acidic and basic compounds on a single standard C18 column. Imtakt's Scherzo SM-C18 and Scherzo SW-C18 HPLC columns can separate acidic ibuprofen and basic chlorpheniramine simultaneously.
For lurasidone treatment adherence testing, an untargeted high-resolution mass spectrometry method was employed, using known positive human urine samples to identify the lurasidone metabolites and their relative abundance in urine.
Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.
Metabolomics, the study of small molecule metabolites that are found within a biological sample, is an emerging field of study. Progress in this field depends upon technological advancement in the fields of LC–MS and separation technology.
Here, the process to build an accurate mass database is thoroughly described and applications are commented on.
This process can improve efficiency in a drug discovery laboratory.
A look at the role of system suitability tests (SSTs) during performance qualification (PQ).