Author


Einar Pontén

Latest:

Advancements in the Understanding of Stationary Phases for HILIC

Some of the newer hydrophilic interaction liquid chromatography (HILIC) columns are reviewed, and experiments performed to categorize columns for optimized separations are described.


Dee Dee Zhang

Latest:

Ghost Peak Investigation in a Reversed-Phase Gradient LC System

Here is a step-by-step approach to investigate the source of ghost peaks in a gradient LC method.


A. Walch

Latest:

Matrix-Assisted Laser Desorption-Ionization Imaging Mass Spectrometry for Direct Tissue Analysis

A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS


Simion Kreimer

Latest:

The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part II: Examples and Comparison to Mass Spectrometry

Detailed examples of light-scattering techniques are presented as well as how their performance compares to MS and other methods.


Gabriela Zurek

Latest:

Analysis of Secondary Metabolites from Myxobacteria using ESI-TOF–MS and PCA

The exploration of myxobacterial metabolite profiles by LC–MS screening for the presence of new natural products is described. Extracts from fermentations of Myxococcus strains are analysed by UPLC-coupled ESI-TOF mass spectrometry and the obtained data are processed using principal component analysis (PCA). The generation of molecular formulae from accurate mass measurements facilitates rapid compound identification.



Evert-Jan Sneekes

Latest:

Nano LC: Principles, Evolution, and State-of-the-Art of the Technique

Nano LC is now widely applied in proteomics, and also is robust enough for application in fields such as pharmaceutical analysis.


GmbH

Latest:

Determination of Explosives Using Fast and High Resolution Liquid Chromatography with the Agilent 1290 Infinity LC System

This application note describes the method for detection of nanogram levels of explosive constituents in seawater samples (e.g., after detonation of unexploded ordnance devices). The improved chromatographic method was developed using the Agilent 1290 Infinity LC Method Development system.


Mark Woodruff

Latest:

The Effect of Particle Monodispersity in HPLC Column Performance

We explore advancements in silica particles for liquid chromatography, focusing on the shift toward improved monodispersity and its impact on chromatographic parameters, efficiency, backpressure, and sensitivity in high-performance liquid chromatography (HPLC).


Tuulia Hyötyläinen

Latest:

Ultrahigh-Performance Liquid Chromatography–Mass Spectrometry in Lipidomics

An analytical method for the global profiling of molecular lipids in biological samples, with particular emphasis on the plasmalogen lipids, is described.


Lukas Kaminski

Latest:

Why System Suitability Tests Are Not a Substitute for Analytical Instrument Qualification (Part 3): Performance Qualification (PQ)

A look at the role of system suitability tests (SSTs) during performance qualification (PQ).


S.A. Schoenbichler

Latest:

Matrix-Assisted Laser Desorption-Ionization Imaging Mass Spectrometry for Direct Tissue Analysis

A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS


Qin Feng

Latest:

Determination of Clenbuterol-Like Beta-Agonist Residues in Hair

A sensitive and selective liquid chromatography spectrometry mass spectrometry (LC–MS–MS) method to determine clenbuterol-like beta agonist residues in human hair was developed and validated.


Baiping Ma

Latest:

Comparison of Extracts from Dry and Alcohol-Steamed Root of Polygonatum kingianum (Huang Jing) by Sub-2-µm–LC–TOF-MS

Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.


Udo Huber

Latest:

Size-Exclusion Chromatography–Mass Spectrometry Analysis of mAbs on a Biocompatible Flow Path

This article details a recent experiment that demonstrates how nonspecific interactions can be prevented by using metal-free or low adsorption flow paths.


B. Pfeifer

Latest:

Matrix-Assisted Laser Desorption-Ionization Imaging Mass Spectrometry for Direct Tissue Analysis

A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS


Norbert Helle

Latest:

Advances in the Ageing Chemistry of Distilled Spirits Matured in Oak Barrels

Describing developments in LC–MS and GC–MS that reveal the ageing chemistry of whiskey aged in oak barrels.



Laurence M. Brill

Latest:

Responding to Data Analysis and Evaluation Challenges in Mass Spectrometry–Based Methods for High-Throughput Proteomics

This new data-management method is specifically designed to handle the large amounts of data produced in proteomics studies. Experimental methods and data are presented from a recent study conducting proteomics profiling of Schizosaccharomyces pombe to demonstrate the effectiveness of the data management solution. The results demonstrate enhanced data search speeds as well as effective data analysis.


Paola Donato

Latest:

Evaluation of PGC Stationary Phases Under High-Temperature LC Conditions for the Analysis of Parabens in Food Samples

This article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.


Michael Balough

Latest:

Visualizing the Chemical Composition of Complex Samples

How does one decipher and tabulate thousands of chemical formulas and relative abundances without losing the important details?


Alun Cole

Latest:

High-Definition Screening for Boar Taint in Fatback Samples Using GC–MS

High-definition screening by gas chromatography–mass spectrometry (GC–MS) is shown to be a viable option for the reliable identification of odorous compounds in pork.


Peter Link

Latest:

Why System Suitability Tests Are Not a Substitute for Analytical Instrument Qualification (Part 3): Performance Qualification (PQ)

A look at the role of system suitability tests (SSTs) during performance qualification (PQ).


Bhavana Verma

Latest:

The Scherzo Family Columns Simultaneously Separate Acidic and Basic Pharmaceutical Compounds and Resolve Better than a Standard C18 Column

Compounds such as ibuprofen, chlorpheniramine, and allantoin are important in the field of pharmaceuticals and cosmetics. The analysis of acidic and basic compounds is particularly important but it is sometimes difficult to analyze both acidic and basic compounds on a single standard C18 column. Imtakt's Scherzo SM-C18 and Scherzo SW-C18 HPLC columns can separate acidic ibuprofen and basic chlorpheniramine simultaneously.


Jeffrey R. Enders

Latest:

Determination of the Relative Prevalence of Lurasidone Metabolites in Urine Using Untargeted HRMS

For lurasidone treatment adherence testing, an untargeted high-resolution mass spectrometry method was employed, using known positive human urine samples to identify the lurasidone metabolites and their relative abundance in urine.


Liping Kang

Latest:

Comparison of Extracts from Dry and Alcohol-Steamed Root of Polygonatum kingianum (Huang Jing) by Sub-2-µm–LC–TOF-MS

Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.


Gary J. Patti

Latest:

Novel C18 Stationary Phase Provides Exceptional Results for Metabolite Profiling

Metabolomics, the study of small molecule metabolites that are found within a biological sample, is an emerging field of study. Progress in this field depends upon technological advancement in the fields of LC–MS and separation technology.


Antonia Garrido Frenich

Latest:

An Accurate Mass Database for the Analysis of Pesticides and Veterinary Drugs using Liquid Chromatography-High-Resolution Mass Spectrometry

Here, the process to build an accurate mass database is thoroughly described and applications are commented on.


Lifu Ma

Latest:

A Generic Workflow for Achiral SFC Purification of Complex Pharmaceutical Mixtures

This process can improve efficiency in a drug discovery laboratory.


Martin Tegtmeier

Latest:

Why System Suitability Tests Are Not a Substitute for Analytical Instrument Qualification (Part 3): Performance Qualification (PQ)

A look at the role of system suitability tests (SSTs) during performance qualification (PQ).