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HPLC for Characterization and Quality Control of Therapeutic Monoclonal AntibodiesThis instalment describes recent advances and best practices in HPLC analysis of intact monoclonal antibodies (mAbs) and their variants and fragments.
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On-Line Two-Dimensional Liquid Chromatography (2D-LC) for the Analysis of PharmaceuticalsOn-line two-dimensional liquid chromatography (2D-LC) embracing mainly comprehensive LC (LC×LC) and multiple heart-cutting LC (mLC–LC) offers new opportunities for in-depth characterization of pharmaceuticals. Reversed-phase LC × reversed-phase LC using different column chemistries and mobile phases provides good orthogonality for a wide range of applications related to small molecule drugs. Moreover, hardware configurations and software are now commercially available to perform LC×LC and mLC–LC measurements in a reproducible manner.
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Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent AcquisitionThis article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
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Screening of Drugs of Abuse Using the Velox 360 Paper Spray SystemPaper spray mass spectrometry (MS) allows for the direct analysis of pharmaceuticals, drugs of abuse, and other small molecules from blood, urine, and other biofluids (1,2).
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Positive-Negative Switching LC–MS–MS for Quantification of Pesticides in JuiceLiquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC–MS–MS) in multiple reaction monitoring (MRM) mode is widely considered as the gold standard for pesticide analysis. In this article recent developments in LC–MS–MS technology to meet increasing regulatory demands and productivity targets in quality control and contract laboratories are explored.
Latest:
Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent AcquisitionThis article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.
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BioPharma Compass: A Fully Automated Solution for Characterization and QC of Intact and Digested ProteinsBioPharma Compass? is a fully automated solution for the rapid characterization of biopharmaceutical products such as proteins, peptides, RNA, and DNA. This push button solution assists nonspecialist operators to generate high quality, accurate data for automatic comparison with laboratory reference standards. Automated, visual reports are then generated for each sample and important information regarding a products purity and identity can be observed at a glance. In this application note, we will apply the BioPharma Compass workflow to the QC characterization of two proteins including intact IgG1 and digested transferrin.
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Lab-on-a-Chip GC for Environmental ResearchThe development of a microfabricated comprehensive GC that can be used as a portable measurement device.
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Positive-Negative Switching LC–MS–MS for Quantification of Pesticides in JuiceLiquid chromatography coupled to triple quadrupole tandem mass spectrometry (LC–MS–MS) in multiple reaction monitoring (MRM) mode is widely considered as the gold standard for pesticide analysis. In this article recent developments in LC–MS–MS technology to meet increasing regulatory demands and productivity targets in quality control and contract laboratories are explored.
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Comparison of Extracts from Dry and Alcohol-Steamed Root of Polygonatum kingianum (Huang Jing) by Sub-2-µm–LC–TOF-MSMost plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.
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The Effect of Particle Monodispersity in HPLC Column PerformanceWe explore advancements in silica particles for liquid chromatography, focusing on the shift toward improved monodispersity and its impact on chromatographic parameters, efficiency, backpressure, and sensitivity in high-performance liquid chromatography (HPLC).
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Chinese American Chromatography Association Provides Networking, Career Support, and a Sense of CommunityWhen one walks into either a chromatography research laboratory at a university or a chromatography testing or R&D laboratory in a North American company, one may notice one common factor: A significant number (more than 50%) of researchers are of Chinese origin. This was not the case 25 years ago.
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Pulsed Electrochemical Detection: Waveform EvolutionDevelopments in pulsed electrochemical detection are traced through the progression of its waveforms.
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Lab-on-a-Chip GC for Environmental ResearchThe development of a microfabricated comprehensive GC that can be used as a portable measurement device.
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Optimizing Sensitivity in GC–FID AnalysisIt is often possible to achieve better sensitivity and lower limits of detection and quantitation using standard gas chromatography instrumentation. Paying attention to fundamental variables-like sample solvent, initial oven temperature hold, carrier gas operating mode, and thermal gradients- can lead to much improved method performance.
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Fluorescence-Detection Size-Exclusion Chromatography — An Analytical Technique with Multiple ApplicationsIn this installment, we discuss fluorescence-detection size exclusion chromatography (FSEC) technology and its implications in protein characterization, specifically the development and characterization of biologics.
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Fluorescence-Detection Size-Exclusion Chromatography — An Analytical Technique with Multiple ApplicationsIn this installment, we discuss fluorescence-detection size exclusion chromatography (FSEC) technology and its implications in protein characterization, specifically the development and characterization of biologics.
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Establishing USP Rebaudioside A and Stevioside Reference Standards for the Food Chemicals CodexHere's how these new reference standards were characterized.
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Advances in the Ageing Chemistry of Distilled Spirits Matured in Oak BarrelsDescribing developments in LC–MS and GC–MS that reveal the ageing chemistry of whiskey aged in oak barrels.
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How Much Pomegranate Is In Pomegranate Juice?Pomegranates are loaded with antioxidants and millions of people turn to pomegranate juice for its many health benefits, but every time you take a sip from a bottle of the popular juice, how much of the actual fruit are you consuming?
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Improving Intact IgG Separations with Aeris WIDEPORE Core-Shell HPLC/UHPLC ColumnsPhenomenex Application Note
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Quantification of Total w-6 and w-3 Fatty Acids and w-6/w-3 Ratio in Human Serum Using GC-MSThis article demonstrates the superiority of the GC-MS approach over spectrophotometry.
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Laser Diode Thermal Desorption Tandem Mass Spectrometry for Simultaneous Quantitation of Metformin and Sitagliptin in Mouse and Human Dried Blood SpotsAn exploration of LDTD-MS-MS and how it compares to HPLC–MS-MS techniques in terms of sensitivity, robustness, and speed in an in-vivo drug discovery application
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An Accurate Mass Database for the Analysis of Pesticides and Veterinary Drugs using Liquid Chromatography-High-Resolution Mass SpectrometryHere, the process to build an accurate mass database is thoroughly described and applications are commented on.
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Flow Path Considerations for Improved SFC AnalysesCertain considerations must be made with regards to the overall ?ow path used in a SFC analysis to ensure additional obstacles are not introduced inadvertently. This article provides more information.
Latest:
Lab-on-a-Chip GC for Environmental ResearchThe development of a microfabricated comprehensive GC that can be used as a portable measurement device.
Latest:
Lab-on-a-Chip GC for Environmental ResearchThe development of a microfabricated comprehensive GC that can be used as a portable measurement device.
Latest:
Quantification of Total w-6 and w-3 Fatty Acids and w-6/w-3 Ratio in Human Serum Using GC-MSThis article demonstrates the superiority of the GC-MS approach over spectrophotometry.
Latest:
Metabolomics Workflows: Combining Untargeted Discovery-Based and Targeted Confirmation Approaches for Mining Metabolomics DataThe metabolomics workflow described here combines untargeted (discovery) quadrupole time-of-flight (Q-TOF) liquid chromatography–mass spectrometry (LC–MS), targeted (confirmation) triple-quadrupole LC–MS-MS, and sophisticated data mining as an effective means to elucidate metabolite changes.
