HPLC

Octadecyl bonded silica gel (C18) is widely used in solid-phase extraction (SPE), but there are many variations including particle size, pore size, surface chemistry, and carbon loading applications.

A change in the mobile phase strength can be a powerful tool to move peaks in a separation.

Here is a step-by-step approach to investigate the source of ghost peaks in a gradient LC method.

Ghost peaks can be tough to eliminate when minor peaks are important. This case study shows how to isolate the source of such peaks.

This year, many HPLC systems focused on facilitating process development, method transfer, and biological analysis.

This process can improve efficiency in a drug discovery laboratory.

Here's how these new reference standards were characterized.

Microextraction by packed sorbent is a sample preparation technique that can be connected on-line with LC or GC systems.

Can pressure alone affect the appearance of a chromatogram?

Often, what you remember most about your career is the great people you met along the way.

Can pressure alone affect the appearance of a chromatogram?

A high performance thin-layer chromatographic (HPTLC) method was developed for the determination of aflatoxin B1 in cereals.

A high performance liquid chromatography (HPLC)–UV method was developed for the analysis of phenylurea herbicides and applied successfully to the analysis of herbicides spiked in three soft drink brands and tap water.

Tips on what to consider when choosing another column to try and solve a tricky separation problem.

A review of the liquid chromatography columns and accessories for analytical and preparative chromatography to be unveiled at Pittcon 2011

What challenges occur when using hydrophilic interaction liquid chromatography (HILIC), and what options are available for analysis?

Comparisons between different C18 columns and the best way to select which one to use

Now in its fourth year, the LCGC Awards have quickly grown into one of the premier honors for both emerging and veteran practitioners of chromatography.

A few simple calculations and estimates can provide insight into a troublesome chromatogram.

High recovery, reproducibility, and cost savings are among the advantages of this environmentally friendly method.

Chiral chromatography is now the fastest way to access large amounts of enantiopure pharmaceutical compounds.

Apply the fundmentals to your problem and the cause may become obvious.

A change in the mobile phase strength can be a powerful tool to move peaks in a separation.

This article describes the operating principles of the direct-electron ionization (EI) interface, which is becoming more popular in many LC–MS applications. Matrix effects and the role of direct-EI as a universal detector for small molecule analysis are also discussed in detail. The advantages and drawbacks of this approach are described and a comparison with atmospheric pressure ionization (API) interfaces is made. The potential of direct-EI is illustrated with a selection of practical applications.

The authors discuss an LC method for the direct analysis of diquat and paraquat in lake water.

A change in the mobile phase strength can be a powerful tool to move peaks in a separation.

Examining the properties of the three most commonly used solvents and how to select the most suitable mobile phase composition.

John Dolan presents some practical pointers to get the most out of your UHPLC system.

This article highlights a number of recent developments in HPLC for polyphenol analysis.

The guest authors describe an optimization procedure to achieve the highest plate count in a given analysis time.