HPLC

A description of how refractive index detectors work and some good practices to get the most out of this powerful detector

Two promising wide-pore stationary phases were recently introduced for the fast and high resolution separations of large biomoleculaes: the recently launched widepore 3.6?m superficially porous particle and the hybrid-type sub 2-?m fully porous particle. This article focuses on the achievable throughout and resolution for the characterization of biomolecules.

How can we tell if an LC method is working properly?

An improved liquid chromatography tandem mass spectrometry (LC–MS–MS) method for the screening and confirmation of clenbuterol in human urine in sports doping applications was developed and validated.

Quinine- and quinidine-derived anion-exchanger chiral stationary phases have proven to be versatile in enantiomer separation of acidic compounds in HPLC. In this article, the authors demonstrate their performance in specific HPLC applications involving enantiomer resolution and topoisomer separation.

How can we tell if a liquid chromatography method is working properly? How can a compendial method be adjusted to speed up the run time?

A preview of HPLC 2013.

The HPLC 2012 conference was abuzz with talk of "slip flow" following the talk given by Mary Wirth of Purdue University about her groundbreaking work involving protein column efficiency. In this interview, Wirth explains how “slip flow†works and what this discovery can contribute to the application of LC-MS to disease research.

Click here to view the complete E-Separation Solutions newsletter from October 11, 2012.

Click here to view the complete E-Separation Solutions newsletter from October 4, 2012.

Two promising wide-pore stationary phases were recently introduced for the fast and high-resolution separations of large biomolecules: the recently launched wide pore 3.6 ?m superficially porous particle and the hybrid-type sub-2-?m fully porous particle. This article focuses on the achievable throughput and resolution for the characterization of biomolecules.

How ethylene-bridged hybrid inorganic-organic (BEH) particles are prepared, and how their special quallities can be used to improve separations.

Some of the newer hydrophilic interaction liquid chromatography (HILIC) columns are reviewed, and experiments performed to categorize columns for optimized performance.

A discussion of new column packing materials– including sub-2-?m particles, superficially porous particles and second-generation monoliths– and their impact on instrumentation and laboratory productivity.

An overview of the principles and equipment required for scaling analytical separations to the preparative scale and considerations for the scale of analyte required.

A closer look at how the solvent chosen for injection can affect the appearance of chromatographic peaks.

Book Review: Introduction to Modern Liquid Chromatography, 3rd Edition.

Unsustainable fishing and habitat destruction is increasing pressure on tropical marine eco-systems. Conservation of coral reef habitats has been prioritized at the expense of other habitats, but the importance of maintaining migration links between habitats has been explicitly recognized during the recent re-zoning of the Great Barrier Reef Marine Park.

A comparison of polymer- and silica-based stationary phases used in hydrophilic interaction chromatography (HILIC) for saccharide analysis.

Click here to view the complete E-Separation Solutions newsletter from September 13, 2012.

LCGC recently spoke with Fred E. Regnier, the John H. Law distinguished professor in the Department of Chemistry at Purdue University (West Lafayette, Indiana) and a member of LCGC's editorial advisory board.

A review of the trends at this year&s symposium, including discussions of column technology, sample preparation, and detector usage.

A simple and sensitive high performance liquid chromatography (HPLC) method with ultra-violet (UV) detection has been developed for the analysis of phenylurea herbicides.

Questions from the e-mail bag are considered this month.

A sensitive and selective liquid chromatography spectrometry mass spectrometry (LC–MS–MS) method to determine clenbuterol-like beta agonist residues in human hair was developed and validated.

A panel of experts give their opinions about the current trends in high performance liquid chromatography (HPLC) instrumentation and how the technology could develop in the future.

Questions from the e-mail bag are considered in this month's instalment.

A look at the role of micro-LC–MS to reduce the environmental impact of chromatography laboratories.

This article describes the GC–MS and LC–MS screening methods developed by the Environment Agency for England and Wales for the analysis of both low-volumn water samples and extracts obtained from various designs of passive samplers.

In honor of LCGC's celebration of 30 years covering the latest developments in separation science, we asked a panel of experts (listed in the sidebar) about the current state of the art of high performance liquid chromatography (HPLC) instrumentation and how the technology will develop in the future.