CESI–MS: An Essential Complement to Nano LC–ESI–MS for Complex Proteome Analysis
Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) is often used for the identification of proteins from complex peptide mixtures made from enzymatic digests of selected proteomes.
Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) is often used for the identification of proteins from complex peptide mixtures made from enzymatic digests of selected proteomes. However, these experiments only represent part of the proteome, as there are many peptides that are not ionized, retained or eluted under these reversed-phase LC–ESI conditions.
Extracting Estrogenic Hormones Using Rotating Disk and Modified Clays
April 14th 2025University of Caldas and University of Chile researchers extracted estrogenic hormones from wastewater samples using rotating disk sorption extraction. After extraction, the concentrated analytes were measured using liquid chromatography coupled with photodiode array detection (HPLC-PDA).
Polysorbate Quantification and Degradation Analysis via LC and Charged Aerosol Detection
April 9th 2025Scientists from ThermoFisher Scientific published a review article in the Journal of Chromatography A that provided an overview of HPLC analysis using charged aerosol detection can help with polysorbate quantification.
Removing Double-Stranded RNA Impurities Using Chromatography
April 8th 2025Researchers from Agency for Science, Technology and Research in Singapore recently published a review article exploring how chromatography can be used to remove double-stranded RNA impurities during mRNA therapeutics production.
