October 29th 2024
Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.
Automated MAb Workflow: From Harvest Cell Culture to Intact Mass Analysis of Variants
May 17th 2013Recombinant monoclonal antibodies (MAbs) are a growing class of therapeutic molecules. Early in the development of MAbs, a large number of harvest cell culture (HCC) samples must be screened for immunoglobulin G (IgG) titer, aggregations, and charge variants. This study describes a two-dimensional (2D) high performance liquid chromatography (HPLC) workflow for afinity, size-exclusion, and ion-exchange analysis.
New Chromatography Columns and Accessories at Pittcon (Part 1)
May 1st 2013Part I of Ron Major's report on new products introduced at Pittson. This month, columns are highlighted, covering all modes of high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), and liquid-phase separations.
Core-Shell Particles: How do they Work?
April 18th 2013Fabrice Gritti of the Department of Chemistry at the University of Tennessee (Knoxville, Tennessee, USA) discusses results from his and Georges Guiochon's research on the kinetic mechanisms of chromatography columns packed with core-shell particles.
Improving the Ion Chromatography Fluid Pathway
April 18th 2013Ion chromatography (IC) is an important analysis tool for chromatographers. Typically, emphasis is placed on the hardware components and chemistry to achieve the ideal level of separation. However, the flow path itself is often overlooked, and there are some relatively simple steps that can be taken to ensure that the system's flow path positively affects the overall chromatographic results achieved.
The LCGC Blog: Retention Time Variability in HPLC
March 26th 2013A number of retention time issues can cause problems in HPLC. Here's advice on how to handle common ones, such as retention time drift, temperature variability, problems arising from column equilibration, and difficulties validating a method.