Tips for Achieving Consistent Performance and Long Life of UHPLC Columns when Using Water-Acetonitrile Gradients with Water Soluble Buffers
Reversed-phase ultra high performance liquid chromatography (RP-UHPLC) columns are the most widely used UHPLC columns in analytical separations. As with other reversed-phase columns, analytes are separated in RP-UHPLC columns due to their differences in the degree of hydrophobic interaction with the stationary phase.
Reversed-phase ultra high performance liquid chromatography (RP-UHPLC) columns are the most widely used UHPLC columns in analytical separations. As with other reversed-phase columns, analytes are separated in RP-UHPLC columns due to their differences in the degree of hydrophobic interaction with the stationary phase. In our bacterial metabolomic studies we used a C18 UHPLC column with MS detection. Due to its smaller particle size packing, the UHPLC column provided a very high efficiency separation. The shorter column length and smaller internal diameter helped to achieve high speed separations and reduced mobile phase solvent consumption, thus decreasing cost and environmental impact.
New Study Investigates Optimizing Extra-Column Band Broadening in Micro-flow Capillary LC
March 12th 2025Shimadzu Corporation and Vrije Universiteit Brussel researchers recently investigated how extra-column band broadening (ECBB) can be optimized in micro-flow capillary liquid chromatography.
