Tips for Achieving Consistent Performance and Long Life of UHPLC Columns when Using Water-Acetonitrile Gradients with Water Soluble Buffers
Reversed-phase ultra high performance liquid chromatography (RP-UHPLC) columns are the most widely used UHPLC columns in analytical separations. As with other reversed-phase columns, analytes are separated in RP-UHPLC columns due to their differences in the degree of hydrophobic interaction with the stationary phase.
Reversed-phase ultra high performance liquid chromatography (RP-UHPLC) columns are the most widely used UHPLC columns in analytical separations. As with other reversed-phase columns, analytes are separated in RP-UHPLC columns due to their differences in the degree of hydrophobic interaction with the stationary phase. In our bacterial metabolomic studies we used a C18 UHPLC column with MS detection. Due to its smaller particle size packing, the UHPLC column provided a very high efficiency separation. The shorter column length and smaller internal diameter helped to achieve high speed separations and reduced mobile phase solvent consumption, thus decreasing cost and environmental impact.
Green Chemistry: What is it (and What Is It Not)? And How Does It Apply to Gas Chromatography?
October 31st 2024Everyone is talking about sustainability, and organizations are creating sustainability programs. But what does green chemistry really mean, and how does it apply to gas chromatography?
HILIC Peptide Retention Times Predicted Using New Approach
October 29th 2024Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.
A Review of the Latest Separation Science Research in PFAS Analysis
October 17th 2024This review aims to provide a summary of the most current analytical techniques and their applications in per- and polyfluoroalkyl substances (PFAS) research, contributing to the ongoing efforts to monitor and mitigate PFAS contamination.
