Biological, Medical, and Clinical Analysis

How to use these tools to develop an efficient and robust process

Detailed examples of light-scattering techniques are presented as well as how their performance compares to MS and other methods.

Sample clean-up using solid-phase extraction (SPE) and similar methods can sometimes be both time-consuming and expensive. The purification of complex plant extracts requires special care in particular. A robust and sensitive online SPE sample preparation method is described for the determination of steviol glycosides.

This work demonstrates the potential of the simultaneous on-line hyphenation of asymmetric flow field flow fractionation (AF4) to inductively coupled plasma-mass spectrometry (ICP-MS) and ultra violet-visible/multi angle light scattering (UV-vis/MALS) detection as a promising and simple tool to obtain information on the multi-element speciation and absolute molecular mass of human plasma proteins such as albumin and transferrin: the two main proteins that have been proposed to bind and transport metal ions in this biological fluid.

Smaller particles, increasingly clever ways to overcome mass transfer limitations, further reductions in plate height, shorter separation times, higher pressure, and enhanced peak capacity dominate thinking in the high performance liquid chromatography (HPLC) community today. For healthcare applications, however, we need to think differently about how to improve analytical separations.

The Column spoke to Martin Giera of Leiden University Medical Centre, Leiden, The Netherlands, about developments in biomarker discovery.

Surfactants present a real challenge to the analytical chemist as they are a very complex class of chemical compounds.

Wyatt Application Note

Biotage Application Note

Wyatt Application Note

CE–MS using noncovalent coated capillaries provides advantages for purity and stability analysis of biopharmaceuticals.

LC–MS and CE–MS are the go-to techniques for characterizing biologics, but often help is needed from a range of other methods.

Biotage Application Note

Agilent Application Note

Waters Application Note

Accurate-mass approaches offer a significant advance over nominal-mass approaches in the arena of qualitative analysis, and some of the analytical approaches can now be conducted in a relatively routine manner.

Turbulent flow chromatography is often used for on-line sample cleanup of biological matrices in liquid chromatography–mass spectrometry applications.

Part I of this series explains how lipids analysis has evolved.

For PAT, you need to choose the right analytical methods. Here's how.

Biotage Application Note

Bruker Application Note

Metrohm Application Note

Biotage Application Note

Spark Application Note

Thermo Fisher Scientific Application Note

Biotage Application Note

Shimadzu Application Note

Tosoh Application Note

A description of the use of UHPLC in the biopharmaceutical industry to characterize drug substances through peptide mapping and amino acid analysis

Until recently, a promising solution to couple capillary electrophoresis to electrospray ionization mass spectrometry has only been available to a small group of experts.