Biological, Medical, and Clinical Analysis

A new study reveals that real-time library searching significantly improves the structural characterization of glycerophospholipids and sphingomyelins, offering enhanced selectivity and comprehensive analysis. By utilizing real-time identifications to trigger targeted MSn analyses, researchers gain deeper insights into these crucial lipid species.

Researchers have discovered diacylglycerols as potent biomarkers for the rapid diagnosis of breast cancer, using mass spectrometry imaging. This breakthrough study offers new possibilities for improved intraoperative assessment and personalized treatment strategies.

Mixed-mode cationic exchange (MCX) sorptive tapes combined with direct infusion mass spectrometry enable selective extraction and rapid analysis of cationic compounds in complex samples, such as opioids in saliva.

The development of a newly-designed Girard's reagent, HBP, as charged isobaric mass tags has enabled selective capture and sensitive detection of aldehyde metabolites. The LC–dNLS-MS/MS screening approach using this reagent has successfully facilitated the discovery of natural aldehydes and their profiling in complex samples.

Researchers have developed an innovative strategy for compound annotation in offline 2D-LC–MS analysis. The approach combines hand-in-hand alignment with targeted molecular networking (TMN) and was successfully applied to analyze the chemical profile of Yupingfeng (YPF), a traditional Chinese medicine prescription, resulting in the identification of 497 compounds and demonstrating its efficiency and scalability in complex sample analysis.

A newly developed UHPLC–MS/MS method allows for the accurate analysis of bioactive compounds, including apigenin, apigenin 7-glucoside, and chlorogenic acid, in goat serum.

This article explores what this transition to newer technology looks like, with an example applied to determining organic impurities of pramipexole dihydrochloride.

Scientists achieve a breakthrough in separation techniques with the development of alizarin-modified iron oxide magnetic nanoparticles, enabling highly efficient and selective separation of anthraquinones from complex plant extracts.

The latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.

Superficially porous particle (SPP) column technology is leveraged to optimize high-throughput HPLC methods for small-molecule drug discovery, resulting in improved efficiency and reduced runtime.

An analytical team has successfully developed a novel strategy for the separation of sanshools from Zanthoxylum bungeanum oleoresin using countercurrent chromatography, providing valuable insights for the separation of compounds with similar chemical properties.

Researchers in Iran utilize graphene oxide aerogels to significantly improve the extraction and analysis of risperidone from plasma samples, offering enhanced accuracy and efficiency in pharmaceutical analysis.

A novel system combining hydrogel-modified human umbilical vein endothelial cell membrane chromatography with high-performance liquid chromatography-mass spectrometry revolutionizes the screening and purification of active ingredients in herbal medicines.

A recent study has introduced comprehensive quality control procedures for large-scale plasma proteomics analyses. The research provides valuable insights into optimizing performance, enhancing reproducibility, and ensuring quantitative accuracy in proteomics investigations.

A novel analytical method combines charge inversion ion-ion reactions with ion mobility–mass spectrometry to enhance the separation and characterization of oligosaccharides.

A new tandem mass spectrometry technique has been developed for the rapid determination of complex lipid A structures, including novel variations from arctic bacterioplankton. This study enhances our understanding of bacterial adaptations and paves the way for the development of targeted therapies and antimicrobial agents.

A cutting-edge technique combining droplet microfluidics and mass spectrometry revolutionizes monoclonal antibody screening, enabling rapid and high-throughput analysis while setting a new standard for efficient clone selection in biopharmaceutical development.

Scientists have developed a new method for predicting and assessing the source of black inks using direct analysis in real-time mass spectrometry. By applying dimensionality reduction techniques and likelihood ratio analysis, the researchers achieved high accuracy in identifying ink sources, demonstrating the potential of this approach in forensic ink analysis.

Metabolic Perturbation Score-Based Mass Spectrometry Imaging (MPS-MSI) offers spatially resolved insights into the functional metabolic response, facilitating a deeper understanding of disease progression and drug action, and paving the way for improved therapeutic strategies and targeted interventions.

A research team has developed a novel method for assessing the volatility of individual components in complex organic aerosol mixtures using temperature-programmed desorption and high resolution mass spectrometry, offering insights into atmospheric pollutants.

Researchers have developed a novel liquid chromatography–mass spectrometry method for analyzing sialic acid linkage in N-linked glycopeptides, offering insights into glycosylation patterns.

A new method determines gas-polydimethylsiloxane (PDMS) distribution constants of key Cannabis terpenes and terpenoids, providing insights into their behavior and for optimizing SPME methods for analysis of these compounds.

Innovative gas chromatography (GC) study combines computer vision and chromatographic fingerprinting to uncover diagnostic signatures in food volatilome.

The evolution of biomolecule analysis is discussed in this article.

A new study reports the development a highly sensitive LC–MS/MS method to measure salivary melatonin levels. The method has a lower limit of the measuring interval of 0.8 pg/mL, making it the most sensitive method for quantifying melatonin in saliva.

A recent study has found that using only seven points across an LC–MS/MS peak can provide sufficient accuracy and precision for drug quantitation studies in drug discovery and development, challenging the "rule-of-thumb" of using at least 15 points.

A recent study analyzed the composition and metabolism of steroidal alkaloids in Solanum nigrum fruits of different maturities and identified steroidal glycoalkaloids and steroidal alkaloids, providing a foundation for further research on biosynthesis and discovery of new steroidal alkaloid anticancer drugs.

A new study has identified potential quality-markers for Eucommiae Folium (Duzhongye), a traditional Chinese medicine, using UHPLC–MS/MS analysis, and has recommended isoquercitrin as a new quality-marker candidate.

A new approach for identifying isomeric and isobaric metabolites has been developed using liquid chromatography (LC) with high-resolution ion mobility spectrometry (IMS) and cryogenic infrared (IR) spectroscopy, allowing for faster, more accurate, and cost-effective metabolomics workflows.

Researchers at Clemson University have developed a computational framework that integrates commercial software components to perform customizable technoeconomic feasibility analyses of monoclonal antibody capture chromatography platforms.