Biological, Medical, and Clinical Analysis

Researchers have developed a new model for achiral chromatography (ACh) that can separate non-racemic mixtures of enantiomers.

A new study evaluated the use of HILIC for fast aqueous species distribution analysis of Nickel(II)-Histidine complex, finding that HILIC is appropriate for investigating coordination metal complexes.

Researchers have now used mass spectrometry imaging to study induced pluripotent stem cells (iPSCs) during the early stages of differentiation.

By modifying polyniobate on a metal-organic framework, scientists have managed to purify Cytochrome C for proteomic analysis using LC–MS/MS.

A recent study analyzed individual hippocampal cells at ultrahigh mass resolution using a FT-ICR mass spectrometer.

Using LC–MS, scientists have developed a new method for testing doping steroids in blood.

Combining liquid chromatography (LC) with high resolution mass spectrometry (HRMS) has led to incredibly detailed results in forensic drug screenings, though not without challenges.

Chemical isotope labeling (CIL) can lead to excellent biological sample analysis when combined with mass spectrometry (MS).

Direct-infusion tandem mass spectrometry (DI-MS/MS) enables researchers to quantify citrate isomers and isobars in biological samples with high accuracy and reliability.

Scientists recently used continuous electrospray ionization mass spectrometry to study what makes the inland taipei snake venom so dangerous.

Using liquid chromatography-tandem mass spectrometry, scientists have found a similarity between the protective functions of the oral permeability barrier and the skin barrier.

A new approach developed by researchers called MetImage can help decode liquid chromatography–mass spectrometry (LC–MS)-based untargeted metabolomics data into multichannel digital images, which is a new approach for using whole metabolome profiles for AI-based clinical applications.

Dipeptide profiles have an important role in detecting disease, and a recent study has made it much more accessible to study them.

A new buffer management system that automates most processes will further how biopharmaceuticals are produced, helping save more lives in the process.

Solid-phase extraction holds an important role in identifying and improving the effectiveness of antibiotics.

By improving fluorescent labeling, scientists can find new ways to help treat and prevent influenza.

The "Deep Proteome" project is helping to advance our understanding of the vast world of human proteomics.

The current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.

This simple, rapid, and accurate HPLC-UV method can facilitate routine therapeutic drug monitoring in neonates.

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.

Compact instrumentation offers important advantages for many workflows, as illustrated by these examples.

Reducing matrix effects during LC–MS/MS bioanalysis is paramount. Improving sample preparation techniques is the best way to combat this issue.

Research conducted in Norway carried out the first screening of legacy and emerging contaminants in multiple tissues of killer whales to shed light on the impact of persistent chemical contaminants in the wild.

GC and TLC methods are demonstrated for quantification of stigmasterol 3-O-β-D-glucopyranoside (S3G), the main active component in the herbal nutraceutical Balanites aegyptiaca, an antihyperglycemic in Egyptian folk medicine.

This concise yet comprehensive overview of sample preparation for bioanalysis looks at sample preparation fundamentals, best practices, and modern trends—all illustrated with a case study.

We present the main analytical techniques for performing functional characterization of biotherapeutic products. Such assessments are particularly critical for biosimilars, where analytical testing must ensure functional comparability with the innovator product.

This is the first article in a four-part series exploring the quantitative assessment of drugs and their metabolites in biological fluids (such as blood, plasma, and urine) and tissue homogenates using liquid chromatography–mass spectrometry (LC–MS).

This article is the first of four on the bioanalysis of small-molecule drugs and metabolites by liquid chromatography–mass spectrometry (LC–MS). In this article, we provide an overview of the fundamentals, workflow, regulations, and modern trends on these quantitative assays.

A simple and rugged method to determine psychoactive drugs using automated SPE–LC–MS/MS is described.

The Column spoke to Martin Giera of Leiden University Medical Center about his innovative research using GC–MS in bioanalysis applications.