Biological, Medical, and Clinical Analysis

A study published in PLoS ONE has provided the most comprehensive protein profile of cerebrospinal fluid to date, using liquid chromatography to identify 2630 proteins, nearly three times the number previously identified.

LGC is to extend its hair drug analysis services in Europe, following an agreement to market a hair test from drug testing company, Psychemedics.

Characterizing biologics through chromatographic analysis.

The analysis of clozapine and norclozapine in plasma using automated on-line sample preparation and LC–MS/MS

Global health and safety concerns now play a key role in driving innovation of liquid chromatography systems and the uptake of advanced LC technology, such as UHPLC.

An HS-GC-FID method for the determination of ethanol and methanol content in biological fluids is presented.

Peas, beans and some other plant seeds contain proteins, called globulins, which have been shown to act as a defence against insects and other herbivores. Coffee beans contain large amounts of globulins, so scientists in Brazil studied the beans to assess their potential for warding off insects.

Two-dimensional polyacrylamide gel electrophoresis reveals regulatory effects of dietary polyunsaturated fatty acid supply on protein expression in early development.

This article features some of the most frequently used HPLC applications for the characterization of biopharmaceuticals.

How the combination of an electroextraction with hollow-fibre liquid-phase microextraction can lead to a selective, rapid method

Explaining the importance of sensitive detection in phthalate testing during GC–MS analysis.

How the combination of an electroextraction with hollow-fiber liquid-phase microextraction can lead to a selective, rapid new method.

Driving under the influence is a serious criminal offense. To prove a suspect guilty calls for solid scientific evidence. The generally accepted method is to accurately measure the blood alcohol content. This article presents a static headspace-gas chromatography method, which offers reliable qualitative analysis, reproducibility, and short run times to allow for high throughput.

Novel analytical methods for the discovery and trace analysis of biochemically active compunds in three main area are described: protein analysis, screening technologies and multidimensional separations.

October 2006. The authors demonstrate the use of multiple-injection affinity capillary electrophoresis (MIACE) and several variations to MIACE to determine binding constants between the glycopeptide antibotics vancomycin, ristocetin, and teicoplanin from Streptomyces orientalis, Nocardia lurida, and Actinoplanes teichomyceticus, respectively, and D-Ala-D-Ala terminus peptides.

LabLogic Systems have been chosen by the Department of Medical Physics and Clinical Engineering at Addenbroke's Hospital in Cambridge, UK to supply the equipment to support the department?s metabolite analysis system for early-phase PET studies.

BioTek Instruments has appointed Millennium Science as their official distributor in New Zealand. Millennium Science will now be responsible for sales and support of the company?s entire line of microplate-based instrumentation and software in New Zealand and Australia.

American life sciences company Promega, has opened a new EuroLab facility in Charbonnières near Lyon, France. The laboratory will be used to develop new applications to address Europe?s scientific needs.

The Biotechnology and Biological Sciences Research Council (BBSRC) is launching a consultation on Next Generation Sequencing Technologies. The council is seeking comments from individuals and organizations from the UK?s academic and industrial research community about the potential impacts that these technologies can have on research in biosciences.

A provider of contract laboratory services and manufacturer of scientific instruments in the US, has opened a new European laboratory and office in Warwickshire, UK.

Achieving Product Ion Confirmation

Faculty of Health Sciences at the University of Copenhagen will open the doors of its new research centre, The Novo Nordisk Foundation Centre for Protein Research. The centre is the result of an historic donation from the Novo Nordisk Foundation, which in 2007 gave the university €80 million for its establishment.

PerkinElmer and LEAP Technologies have entered into a resale agreement for ultra high performance liquid chromatography (UHPLC) and high performance liquid chromatography (HPLC) technology.

Presented here are four types of evaporation-free extractions that are widely applicable.

A rapid and simple high performance liquid chromatography (HPLC) method with basic extraction assays was developed to investigate free diazepam levels in the plasma and urine samples of patients medicated with this drug for the management of alcohol withdrawal syndrome. The HPLC analysis was optimized and evaluated for linearity, imprecision, recovery, detection and quantification limits. The method showed linearity between 50–500 ng/mL (r2 ≥ 0.990). Coefficients of variations (%CV) were calculated to be in the range of 1.77–9.60. According to ICH guidelines, theoretical limits of detection (LOD) and quantification (LOQ) for plasma and urine were calculated as 8.3 ng/mL, 27.5 ng/mL and 8.2 ng/mL, 26 ng/mL respectively. Diazepam monitoring in plasma and urine displayed remarkable variations. The importance of adjusting doses according to individual requirements and the routine monitoring of plasma or urine for patients under medication is highlighted.

This column discusses the similarities and differences between the validation approaches for analytical methods for QC product analysis and bioanalysis.

ERLIC is a new technique that shows promise as an alternative or complementary technique for both enrichment and separation of these posttranslationally-modified peptides.

The authors examine MS-based methods to search for protein biomarkers of prion diseases from plasma samples.

Guest authors show how mixed modes can be used successfully in the optimization of protein purification, and discuss how various experimental parameters can be used to regulate the binding of proteins to mixed-mode sorbents.

Microextraction by packed sorbent (MEPS) is a new technique in sample preparation that can be connected on-line to gas chromatography (GC) or liquid chromatography (LC) without any modifications.