Biological, Medical, and Clinical Analysis

A new approach for identifying isomeric and isobaric metabolites has been developed using liquid chromatography (LC) with high-resolution ion mobility spectrometry (IMS) and cryogenic infrared (IR) spectroscopy, allowing for faster, more accurate, and cost-effective metabolomics workflows.

Researchers at Clemson University have developed a computational framework that integrates commercial software components to perform customizable technoeconomic feasibility analyses of monoclonal antibody capture chromatography platforms.

The level of uncertainty provided by most forensic laboratories for reported blood alcohol results has been woefully underassessed. Not only is this bad science, but someone’s civil liberties may be at stake.

Researchers combine a single-probe single cell mass spectrometry technique with a bioinformatics software package to quantify cell subpopulations and prioritize metabolite biomarkers, providing a label-free approach to studying cell heterogeneity and improving understanding of cell metabolism in human diseases and response to therapy.

Researchers have developed a PAMAM-functionalized paper substrate that boosts the sensitivity of protein analysis by paper spray mass spectrometry (PS-MS), potentially revolutionizing clinical diagnosis of diseases.

A new study analyzed the trophic niches and niche overlap between native and introduced cyprinid fishes in Karacaören I reservoir using IRMS.

Researchers have developed a new method for detecting pyrethroid pesticide residues in tobacco using Schiff base networks (SNWs) coated stir bar sorptive extraction (SBSE) coupled with high performance liquid chromatography-ultraviolet detection (HPLC-UV), which exhibited high extraction efficiency and good mechanical stability even when reused more than 50 times.

A new RPLC–MS method can map the substitution degree and composition of β-glucose monomers of cellulose ethers (CEs), providing detailed compositional distributions and constitutional isomer distributions, thereby identifying compositional variation between bio-stable and non-bio-stable CEs and unexpected methylations in EHEC samples.

The combination of an untargeted approach using ultrahigh pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF) and a targeted approach using UHPLC–tandem mass spectrometry (MS/MS) are presented as an ideal method for detecting per- and polyfluroalkyl substances (PFAS) in fast-food packaging.

Researchers have shown the effectiveness of ion chromatography (IC) in identifying markers of forest fire emissions in rainfall samples, providing valuable insights into the signature of forest fire emissions and aiding in the development of models to predict and control air pollution resulting from these events.

A review article describes spatial metabolomics using high spatial resolution mass spectrometry imaging (MSI), a promising technology that enables the visualization and analysis of metabolites at the cellular and subcellular levels.

Recent advancements in electrospray ionization mass spectrometry (ESI-MS) for single-cell proteomics are highlighted in a review article, including various separation techniques and MS instrumentation used to improve sample throughput and coverage.

Scientists propose a gel-free hybridization assay using peptide nucleic acid (PNA) probes and capillary electrophoresis (CE) for qualitative and quantitative analysis of nucleic acid therapeutics.

Researchers have developed a new model for achiral chromatography (ACh) that can separate non-racemic mixtures of enantiomers.

A new study evaluated the use of HILIC for fast aqueous species distribution analysis of Nickel(II)-Histidine complex, finding that HILIC is appropriate for investigating coordination metal complexes.

Researchers have now used mass spectrometry imaging to study induced pluripotent stem cells (iPSCs) during the early stages of differentiation.

By modifying polyniobate on a metal-organic framework, scientists have managed to purify Cytochrome C for proteomic analysis using LC–MS/MS.

A recent study analyzed individual hippocampal cells at ultrahigh mass resolution using a FT-ICR mass spectrometer.

Using LC–MS, scientists have developed a new method for testing doping steroids in blood.

Combining liquid chromatography (LC) with high resolution mass spectrometry (HRMS) has led to incredibly detailed results in forensic drug screenings, though not without challenges.

Chemical isotope labeling (CIL) can lead to excellent biological sample analysis when combined with mass spectrometry (MS).

Direct-infusion tandem mass spectrometry (DI-MS/MS) enables researchers to quantify citrate isomers and isobars in biological samples with high accuracy and reliability.

Scientists recently used continuous electrospray ionization mass spectrometry to study what makes the inland taipei snake venom so dangerous.

Using liquid chromatography-tandem mass spectrometry, scientists have found a similarity between the protective functions of the oral permeability barrier and the skin barrier.

A new approach developed by researchers called MetImage can help decode liquid chromatography–mass spectrometry (LC–MS)-based untargeted metabolomics data into multichannel digital images, which is a new approach for using whole metabolome profiles for AI-based clinical applications.

Dipeptide profiles have an important role in detecting disease, and a recent study has made it much more accessible to study them.

A new buffer management system that automates most processes will further how biopharmaceuticals are produced, helping save more lives in the process.

Solid-phase extraction holds an important role in identifying and improving the effectiveness of antibiotics.

By improving fluorescent labeling, scientists can find new ways to help treat and prevent influenza.

The "Deep Proteome" project is helping to advance our understanding of the vast world of human proteomics.