Technical Note: Modifying a Standard HPLC Autosampler for On-Line Process Monitoring
Autosamplers for most high performance liquid chromatography (HPLC) systems share characteristic design features: enclosure in relatively small units and sampling operations performed with samples placed inside the autosampler housing. Although these features suffice for most HPLC applications, they limit the versatility of the autosampling operation when sample containers cannot fit inside the unit. In this technical note, the authors describe a simple modification of the standard HPLC autosampler flow path that enables automated sampling and analysis of samples outside the autosampler unit. An added benefit of the modified configuration is that it does not compromise operation in the standard mode, and switching between the two configurations is easy.
LCGC 17(9), 862–864 (1999).
LCGC’s Year in Review: Highlights in Liquid Chromatography
December 20th 2024This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Next Generation Peak Fitting for Separations
December 11th 2024Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.
Mobile Phase Buffers in Liquid Chromatography: A Review of Essential Ideas
December 11th 2024In this installment of "LC Troubleshooting," Dwight Stoll discusses several essential principles related to when and why buffers are important, as well as practical factors, such as commonly used buffering agents, that are recommended for use with different types of detectors.
