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Optimization of Determination Method of Cooling Agents in Cigarette Tipping Paper by Gas ChromatographyThis GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.
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A Final Word from Incognito—The Past, Present, and Future of ChromatographyAfter 14 years in print, Incognito’s last article takes a look at what has changed over a career in chromatography, but it predominantly focuses on what the future might hold in terms of theory, technology, and working practices.
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Gas Chromatography Tandem Mass Spectrometry Analysis of Ethylene Oxide: An Emerged Contaminant in Seeds and SpicesTwo alternative approaches based on gas chromatography coupled to tandem mass spectrometry (GC–MS/MS) were developed to control the maximum residue limit of the banned insecticide, ethylene oxide (EtO), and its transformation product, 2-chloroethanol (2-CE).
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Polysorbate Analysis in Biopharmaceutical Applications—A Snapshot of the Analytical ToolboxAn overview of different approaches for the qualitative and quantitative analysis of polysorbates.
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Supercritical Fluid Chromatography Coupled with Drift Time Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry as a Tool for Lipid Characterization of HepG2 CellsLipids in SFC are mainly separated according to their lipid classes, which simplifies quantification with standards. In this study, SFC coupled with drift time IM‑QTOF-MS was used to characterize the HepG2 lipidome.
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Accelerating Sample Preparation for the Analysis of Complex SamplesGiven that sample preparation consumes over two thirds of analysis time, sample preparation becomes the bottleneck issue in analytical chemistry, resulting in the urgent necessity of developing accelerated sample preparation techniques.
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Improved Performance of Ultrahigh-Pressure Liquid Chromatography–Mass Spectrometry (UHPLC–MS) Hyphenated SystemsAn UHPLC–MS research prototype instrument was built to improve the resolution power and the usability of conventional LC–MS hyphenated instruments for routine analyses in pharmaceutical applications.
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Enhancing the Performance of Analytical Methods to Combat Doping in SportIon mobility–mass spectrometry (IM–MS) has become a cornerstone of bioanalytical laboratories. New developments could lead to its widespread adoption for regulated bioanalysis methods such as anti-doping testing for anabolic steroids in athletes.
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Automated Hydroxyl Radical Protein Footprinting in a 96-Well FormatThis poster describes GenNext’s first-in-class instrument for fully automated protein footprinting that delivers highly precise and accurate quantification for numerous higher order structure (HOS) mapping experiments.
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Organochlorine Pesticide Analysis Using DiLLMEThis technique achieves highly reproducible and fast analysis of organochlorine pesticides in water by coupling automation with the use of a disperser solvent.
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Multi-Attribute Monitoring of Therapeutic mRNA by Liquid Chromatography–Mass SpectrometryA novel method to simultaneously characterize the capping and poly(A) tail for therapeutic mRNA in a single sample preparation workflow is described.
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Innovations and Strategies of Sample Preparation Techniques to Reduce Matrix Effects During LC–MS/MS BioanalysisReducing matrix effects during LC–MS/MS bioanalysis is paramount. Improving sample preparation techniques is the best way to combat this issue.
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A Well-Written Analytical Procedure for Regulated HPLC TestingThis paper describes the content of a well-written analytical procedure for regulated high-performance liquid chromatography (HPLC) testing. A stability-indicating HPLC assay for a drug product illustrates the required components for regulatory compliance, including additional parameters to expedite a laboratory analyst’s execution.
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Validation of an Aqueous Normal Phase Chromatography Method for the Analysis of Ergothioneine in Commercial MushroomsThe authors developed a new analytical HPLC method using a silica hydride-based column to analyze mushrooms.
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Evaluating the Use of Continuous ChromatographyThe editors of BioPharm International spoke with Christine Rozanas, PhD, global product marketing manager at Cytiva, to discuss the advantages and persistent challenges with implementing continuous downstream chromatography.
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Passing the Test: Understanding Proficiency TestingLaboratories use proficiency tests (PTs) to comply with their accreditation requirements and evaluate analysts’ performance. Laboratories regard PTs as a burdensome chore that must be successfully completed to satisfy internal or external compliance or accreditation requirements. PTs are an integral part of a quality management system (QMS) under quality assurance and control (QA/AC). Understanding the core components of the QMS is an important part of passing any PT test. Unacceptable PT results may have little to do with the result itself but reflect the use and application of statistics, standards, and methods.
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Bioanalysis of Small-Molecule Drugs and Metabolites in Physiological Samples by LC–MS, Part 1: An OverviewThis is the first article in a four-part series exploring the quantitative assessment of drugs and their metabolites in biological fluids (such as blood, plasma, and urine) and tissue homogenates using liquid chromatography–mass spectrometry (LC–MS).
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In Search of The Needle in The Mariana Trench: Host Cell Proteins and the Problem of Dynamic RangeThis article explores the analytical challenges associated with HCP monitoring and reviews recent advances in HCP characterization, with special emphasis on high performance liquid chromatography mass spectrometry (HPLC–MS)-based methods and HCP enrichment techniques.
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A Novel Mass Spectrometry Paradigm to Analyze Classical and New Biotherapeutic ModalitiesA new fragmentation approach—tunable electron-activated dissociation (EAD) tandem mass spectrometry (MS/MS)—offers a solution to better improve the detection and characterization of impurities in lipid nanoparticles and impurities in other biopharmaceutical modalities.
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Go With the Flow: Thinking About Carrier Gas Flow in GCSuccessful GC analysis requires careful control of carrier gas. Here, we explain how to measure and control flow rate, use constant pressure vs. constant flow, and more.
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Best of the Week: EAS 2024 Awardees, Oligonucleotides in Drug Development, and MoreHere is some of the most popular content posted on LCGC International this week.
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Simple Purification and Ultrahigh-Pressure Liquid Chromatography–Tandem Mass Spectrometry Determination of Five Quinoxaline 1,4-dioxides and their Metabolites in Swine LiverThe UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.
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Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
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Suspect Screening of Lupin-Produced Phytotoxins in Environmental SamplesThe authors introduce a new high-throughput approach for analyzing environmental micropollutants.
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Determination and Validation of a Single Laboratory Method for Coenzyme Q10 (Ubiquinone) by UHPLCA fast ultrahigh-pressure liquid chromatography (UHPLC) method, with low solvent consumption, is described for the determination of coenzyme Q10 in a variety of complex formulations. This method exhibits good linearity, reproducibility, accuracy, recovery, and specificity, while resulting in an acceptable limit of detection (LOD) and limit of quantification (LOQ).
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Using the Three Cs of Data Visualization as a Life Raft When You’re Drowning in Multidimensional Chromatography PeaksCorrelation, clustering, and color projection techniques exploit the ability of the human brain to identify patterns from huge amounts of visual information. This process can provide a life raft for a weary chromatographer who is drowning in data.
