Author


Clinton Corman

Latest:

Insights into the Shape-Selective Retention Properties of Porous Graphitic Carbon Stationary Phases and Applicability for Polar Compounds

When the unique shape-selective properties of PGC are appropriately identified, PGC stationary phases can be harnessed for fine separations of structurally related compounds.


Giulio Lievore

Latest:

The Role of Adsorption and pH of the Mobile Phase on the Chromatographic Behavior of a Therapeutic Peptide

We explore the impact of two different stationary phases and ion-pair reagents on the retention behavior of a therapeutic peptide using reversed-phase liquid chromatography. This information is of fundamental importance for the development of reliable, selective, and fast analytical methods able to separate and identify the target peptide.


Martina Catani

Latest:

When van Deemter Goes Upside Down in Chiral Chromatography

The occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.


Drago Kocsar

Latest:

In Vino Veritas: LC–MS in Wine Analysis

Liquid chromatography–mass spectrometry (LC–MS) is a popular technique for the analysis of wine. This article gives an overview of wine analysis and new insights this technique has revealed regarding the composition of wine, possible health benefits, customer safety and the understanding of winemaking processes.


Melissa Churley

Latest:

Fast Semivolatiles Analysis Harmonized for EPA Methods 625.1 and 8270E Using Supported Liquid Extraction and GC–MS/MS

A supported liquid extraction (SLE) and fast gas chromatography–tandem mass spectroscopy (GC–MS/MS) method, used in multiple reaction monitoring (MRM) mode, was developed for the analysis of semivolatile organic compounds (SVOCs) in environmental samples, according to the updated EPA Methods 625.1 and 8270E. This method requires minimal sample handling and yields significant throughput and productivity gains in the laboratory.


François Vallée

Latest:

Evaporation-Free Extraction and Application in High-Throughput Bioanalysis by LC–MS-MS

Evaporation-free extraction (no drying down) is highly desirable because of its reduced cost and pollution, higher speed, and less possibility for contamination and conversion. Presented in this article are four types of evaporation-free extraction that are widely applicable. The first one is for the determination of didanosine over the range of 25.02–2502.00 ng/mL by injecting solid-phase extraction (SPE) eluate directly. The second is for the determination of betamethasone phosphate over the range of 2.51–250.60 ng/mL by injecting SPE eluate after pH adjustment. The third is for the determination of sumatriptan over the range of 0.99–99.40 ng/mL based upon SPE with high organic washing and low organic elution. The fourth is based upon automated dilution after protein precipitation for the determination of raloxifene-4'-glucuronide and raloxifene-6-glucuronide over the ranges of 2.02–202.40 ng/mL and 0.40–39.95 ng/mL, respectively.





Brian De Borba

Latest:

Fast Determination of Anthocyanins to Authenticate Pomegranate Juice

Anthocyanins are a sub-class of naturally electron deficient and powerful antioxidants called flavonoids that are responsible for the red, orange, and blue coloration in fruits and flowers.


Sareeta Nerkar

Latest:

Pickering Laboratories - Analysis of Mycotoxins in Hemp and Hemp- Containing Edible Products

Pickering Laboratories developed an easy and sensitive method to analyze aflatoxins B1, B2, G1, G2, and ochratoxin A in hemp and hemp-containing edible products. Mycotoxins are isolated using immunoaffinity clean-up columns and analyzed with fluorescence detection. To increase sensitivity of aflatoxins B1 and G1, an in-line photochemical reactor (UVETM) is installed before the detector. This method utilizes standard HPLC equipment and allows laboratories to easily determine these mycotoxins at low ppb levels.


Saphwan Al-Assaf

Latest:

Characterization and Prediction of Emulsification Performance of Acacia Gums

The authors use GPC coupled online to multiangle laser light scattering, refractive index, and ultra violet (UV) detectors for the characterization of Acacia gum (gum arabic).


Pranathi Perati

Latest:

Dionex - Peptide Mapping of Ovalbumin

Dionex - Peptide Mapping of Ovalbumin


Cynthia Bosnak

Latest:

The Benefits of ICP-MS for the Determination of Toxic and Nutritional Elements in the Cannabis Family of Flowering Plants

This study will focus on an ICP-MS sample preparation procedure and analytical methodology optimized for both toxic and nutritional elements in dried hops, a surrogate for the cannabis family of flowering plants. It will show that the wide dynamic range of the technique allows it to be used for the simultaneous determination of parts per billion (ppb) levels of heavy metals including Pb, As, Cd and Hg, together with high parts per million (ppm) levels of nutritional elements, such as P, Ca, K and Mg.


Rick Lake

Latest:

Automated Solid Phase Extraction for Efficient Extraction of Fluoroquinolone Antibiotics from Fish

The global demand for fish as a natural source of fresh animal protein, essential fats, minerals, and vitamins continues to rise with the human population.


Dafydd Milton

Latest:

The Use of Temperature to Eliminate Organic Solvent in Analytical HPLC Methods

Increased temperature has been used to assist the elimination of organic modifier required in the mobile phase, to achieve analyte separation in pure aqueous mobile phases.


Chris Loran

Latest:

Quantitative Analysis of Pseudoephedrine Tablets by UHPLC–MS

An effective UHPLC–MS method for high throughput separation, identification and quantification of pseudoephedrine was developed on a Hypersil GOLD PFP 1.9 µm, 2.1 Ã- 100 mm column.


Jeremy Shia

Latest:

Rapid, Specific Analysis of Melamine and Cyanuric Acid in Infant Formula

This method is rapid and sensitive for the analysis of melamine and cyanuric acid simultaneously in infant formula. Using two Oasis solid-phase extraction protocols and the ACQUITY UPLC, the results are consistent with the published US FDA interim method, while demonstrating a reduced analysis time.



Matthew J. Woodcock

Latest:

HPLC Analysis of Non-volatile Analytes Using Charged Aerosol Detection

A detection method based upon aerosol charging was examined for its applicability and performance with high performance liquid chromatography.



Jonathan Beck

Latest:

Fast and Accurate Determination of Algal Toxins in Water Using On-line Preconcentration and UHPLC–HRAM–MS

The development of a column-switching technique based on on-line preconcentration and HRAM–MS to obtain fast and accurate results for the determination of algal toxins in drinking water is discussed.



J. Bauer

Latest:

Application of a GPC-LC–MS/MS Method for the Determination of 31 Mycotoxins in Edible OIls

Gel Permeation Chromatography (GPC) is widely used for sample clean up in mycotoxin analysis. The most commonly described methods use GPC columns packed with SX-3 BioBeads suitable for cleaning Zearalenone, Aflatoxins, and Trichothesenes from edible oils and fatty matrices. Separation of Fumonisins from the oil fraction are inadequate with this column.



Peter Földi

Latest:

Gradient Performance Tests for Reversed-Phase and Biochemical Applications using High Salt Buffers

In gradient mode, the quality of the analysis will be influenced by the performance of the proportioning and mixing of the eluents at any time during a given chromatogram.


Ewa Pruszkowski

Latest:

The Benefits of ICP-MS for the Determination of Toxic and Nutritional Elements in the Cannabis Family of Flowering Plants

This study will focus on an ICP-MS sample preparation procedure and analytical methodology optimized for both toxic and nutritional elements in dried hops, a surrogate for the cannabis family of flowering plants. It will show that the wide dynamic range of the technique allows it to be used for the simultaneous determination of parts per billion (ppb) levels of heavy metals including Pb, As, Cd and Hg, together with high parts per million (ppm) levels of nutritional elements, such as P, Ca, K and Mg.


James W. Jorgenson

Latest:

Recent Advances in Ultrahigh-Pressure Liquid Chromatography

Ultrahigh-pressure liquid chromatography (UHPLC) is a technique that is experiencing continued growth due to the benefits in separation power and speed of analysis over traditional HPLC. We attempt here to give an overview of some of the advances that have occurred in UHPLC since 2003, from the standpoint of both fundamental research and the introduction of commercially-available technology.


Axel Zerrath

Latest:

Mass Analysis from Kilodaltons to Megadaltons Using Macroion Mobility Spectrometry

Mass spectrometry (MS) has advanced to analyze ever-larger biomolecules with the invention of soft ionization techniques like electrospray ionization (ESI). Although ESI has provided a method of generating ions of high mass, mass spectrometers generally suffer both lower sensitivity and lower resolution as the mass-to-charge ratio of an ion increases. To extend the mass range of ionized macromolecules beyond the limits of MS, macroion mobility spectrometry utilizes ion mobility sizing to characterize charge-reduced ESI-generated macroions from >5 kDa to beyond megadalton masses. One prominent application of macroion mobility spectrometry, highlighted here, is the high sensitivity analysis of intact proteins, antibodies, and conjugates in which molecular masses range from antibody light-chain fragments to high mass immunoglobulin multimers.


G. Engelhardt

Latest:

Application of a GPC-LC–MS/MS Method for the Determination of 31 Mycotoxins in Edible OIls

Gel Permeation Chromatography (GPC) is widely used for sample clean up in mycotoxin analysis. The most commonly described methods use GPC columns packed with SX-3 BioBeads suitable for cleaning Zearalenone, Aflatoxins, and Trichothesenes from edible oils and fatty matrices. Separation of Fumonisins from the oil fraction are inadequate with this column.