This article describes a comprehensive study on optimizing ion-pair reversed-phase liquid chromatography for analysing protected and unprotected single-stranded DNA oligonucleotides.
Integrating electric fields into extraction techniques increases sensitivity and selectivity. These approaches are compatible with miniaturized, portable, and many biological samples.
Monoliths are chromatography sorbents cast into columns as a single continuous piece in contrast with regular chromatographic sorbents, which are packed as individual particles. The guest authors compare three such novel sorbents with a conventional particle-packed column.
Commercially available trypsin IMERs can digest proteins with high sequence coverage and robustness, facilitating online multidimensional LC–MS.
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Guest author Reg Cross discusses strategies for collecting samples from large masses of material. He also describes the problems associated with acquiring truly representative samples for analysis.
This GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.
The authors compare their results in analyzing styrene–butadiene block copolymers by gel permeation chromatography with other methods, such as Fourier transform infrared spectroscopy and pyrolysis gas chromatography.
October 4, 2021 at 3pm BST | 10am EST | 4pm CEST Join global industry and regulatory experts as they discuss current hot topics in extractables and leachables (E&L) testing for pharmaceuticals, biopharmaceuticals and medical devices.
Using ion mobility, analytes that have the same molecular mass can be separated by their shape, centers of mass, and collision cross section, but challenges such as ion loss can still occur. A new development in ion mobility separation, high-resolution ion mobility (HRIM), addresses such problems, and is particularly well suited to challenging applications, such as glycosylation monitoring of biological drugs and vitamin D analysis.
Per- and polyfluoralkyl substances (PFAS) are found in our food. Sensitive, precise, and accurate analytical methods are needed to estimate human exposure to these chemicals. A comparative study was performed between two extraction and cleanup methods (solid-phase extraction [SPE] and dispersive SPE) for the analysis of PFAS in apples. Both methods showed excellent sensitivity, precision, and accuracy. dSPE has some benefits over conventional SPE, and vice versa. The advantages and disadvantages of both methods are discussed.
Hydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.
A column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
HRIM has emerged as a robust separation strategy for complex chemical analyses due to its ability to improve peak capacity and aid in the separation of isobaric signals.
Pyrolysis–gas chromatography–mass spectrometry has advantages for the analysis of environmental microplastic samples compared to other leading analytical methods, including spectroscopic techniques.
This GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.
Food contamination from mineral oil saturated hydrocarbons (MOSHs) and mineral oil aromatic hydrocarbons (MOAHs) is problematic and requires a sensitive analytical technique. These contaminants were analyzed using GC×GC with flame ionization detection (FID) and time-of-flight–MS (TOF–MS) parallel dual detection. The method provides enhanced chromatographic separation, along with the full mass spectra information, and overcomes difficult interferences, resulting in reduction of false positives over conventional GC–MS methods.
Improved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.
The current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.
The success of screening column and mobile phase combinations that generate dissimilar selectivity is highlighted in a typical method development strategy.
This information is supplementary to the article “Non-aqueous Ion-Exchange Chromatography Using High Acid‑base Concentration: A Strategy for Purifying Non-crystalline Pharmaceutical Intermediates” that was published in the March 2022 issue of LCGC Asia Pacific.
A collaborative multi-analyte method developed utilizes HPLC and UHPLC to analyze more than 70 active ingredients. This method offers a validated approach for determining technical AI alongside linearity, precision, accuracy, and specificity tests for seven active ingredients.
HS-SPME-GC–MS was combined with OPLS-DA data analysis to tentatively identify eight chemical markers to differentiate the geographical origins of cigar leaf samples.
Pepsin digestion and guanidine hydrochloride post-digestion can improve sequence coverage in antibody peptide mapping compared with trypsin digestion.
The latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.
The UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.
The current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.
The challenges that arise during cannabis metabolomics analysis using ultrahigh-performance reversed-phase liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC–reversed-phase–HRMS/MS) are presented.
Compact instrumentation offers important advantages for many workflows, as illustrated by these examples.