Author
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Development of Gas Chromatography Instruments at ShimadzuThe guest authors summarize the history of this Japanese scientific instrument company and events in the development of its products.
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Enter the Matrix: Improving the Interpretation of Separations Data Using Chemometrics in Analytical InvestigationsExamples from food science illustrate the usefulness of chemometrics techniques like pattern recognition, regression, and classification.
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Portable and Field‑Deployable Liquid Chromatography for Environmental StudiesLiquid chromatography (LC) is a platform technology amenable to portable and “at-site” or deployable applications. This has awoken end-users to new possibilities and potential cost savings and process improvements.
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Assessing How Chemical Exposures Affect Human HealthMeasuring chemical exposure is extremely challenging due to the range and number of anthropogenic molecules encountered in our daily lives, as well as their complex transformations throughout the body. To broadly characterize how chemical exposures influence human health, a combination of genomic, transcriptomic, proteomic, endogenous metabolomic, and xenobiotic measurements must be performed. However, while genomic, transcriptomic, and proteomic analyses have rapidly progressed over the last two decades, advancements in instrumentation and computations for nontargeted xenobiotic and endogenous small molecule measurements are still greatly needed.
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Future-Proofing Validated Pharmacopeia Methods: A Case Study of Improving Throughput and Decreasing Operational CostHow to modernize an older pharmaceutical monograph from an HPLC column to an UHPLC column, along with calculated time and solvent savings.
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Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry (HS-SPME-GC–MS) for the Characterization of Cigar LeavesHS-SPME-GC–MS was combined with OPLS-DA data analysis to tentatively identify eight chemical markers to differentiate the geographical origins of cigar leaf samples.
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Application of Liquid- and Supercritical Fluid Chromatography Coupled with High-Resolution Mass Spectrometry for the Analysis of Short-, Medium-, and Long-Chain Chlorinated Paraffins in Dietary SupplementsScientists used supercritical fluid chromatography (SFC) and ultrahigh-pressure liquid chromatography (UHPLC)—both coupled with high-resolution mass spectrometry (HRMS)—to analyze various types of chlorinated paraffins (CPs) in fish oil-based dietary supplements.
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Achieving Success in a Career as a Separation Scientist: An Overview of the Chinese American Chromatography Association (CACA) Career Development Workshop at PittconSince 2008, the Chinese American Chromatography Association (CACA) has hosted career development workshops at Pittcon, featuring over 65 well-established pharmaceutical, biotechnology, and contract research organizations, and academia professionals. This year’s workshop featured five speakers, all of whom shared valuable insights and advice for individuals at different stages of their careers, including graduate students, early-career professionals, high-level managers, and aspiring entrepreneurs.
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Cryogenic- and Flow-Modulation Comprehensive Two-Dimensional Gas Chromatography–Mass Spectrometry: Obtaining Similar Chromatography PerformancesIn the present research, similar chromatography fingerprints were obtained using finely-tuned cryogenic-modulation (CM) and flow-modulation (FM) comprehensive two-dimensional gas chromatography–mass spectrometry (GC×GC–MS) experimental conditions.
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Fundamentals of Benchtop GC–MS Data Analysis and TerminologyIn this installment, we will review the fundamental terminology and data analysis principles in benchtop GC–MS. We will compare the three modes of analysis—full scan, extracted ion chromatograms, and selected ion monitoring—and see how each is used for quantitative and quantitative analysis.
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AI-Powered Precision for Functional Component Testing in Tea AnalysisAnalyzing functional foods reveals numerous health benefits. These foods are rich in bioactive compounds that go beyond basic nutrition, boosting the immune system and improving overall wellness. However, analyzing these compounds can be challenging. This article discusses AI algorithms to support automated method development for liquid chromatography, simplifying the process, enhancing labor efficiency, and ensuring precise results, making it accessible to non-experts for tea analysis.
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In Bruges: HPLC 2025Here's a taster of what to look forward to at the 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025), which will be held from Sunday through Thursday, 15–19 June, 2025, in Bruges, Belgium.
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The Role of Adsorption and pH of the Mobile Phase on the Chromatographic Behavior of a Therapeutic PeptideWe explore the impact of two different stationary phases and ion-pair reagents on the retention behavior of a therapeutic peptide using reversed-phase liquid chromatography. This information is of fundamental importance for the development of reliable, selective, and fast analytical methods able to separate and identify the target peptide.
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Development of a “Freeze-Pour” Sample Preparation Method for the GC Analysis of Semivolatile Flavouring Chemicals Present in E-cigarette Refill LiquidsHS-GC analysis is presented as an excellent method for the analysis of high volatile components in e-liquids. For the analysis of semivolatile ingredients, an additional sample preparation step is proposed based on a LLE followed by a freeze-out of the matrix components.
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An Overview of the Untargeted Analysis Using LC–MS (QTOF): Experimental Process and Design ConsiderationsUntargeted analysis using liquid chromatography with tandem mass spectrometry (LC–MS/MS) chemical profiling is a valuable tool, but care needs to be taken with experimental design.
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How Tube-Based Thermal Desorption Can be Used to Monitor Chemical Warfare Agents at Ultratrace LevelsThis study demonstrates the analysis of live chemical warfare agents and simulants at trace and higher levels.
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In Memory of Pavel Jandera, Leading ChromatographerProfessor Pavel Jandera passed away suddenly and unexpectedly on August 5, 2021. He was one of the most famous Czech analytical chemists. He was 77 years old.
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Systematic Evaluation of HILIC Stationary Phases for MS Characterization of OligonucleotidesHydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.
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Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography MethodsA column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
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An Overview of the Untargeted Analysis Using LC–MS (QTOF): Experimental Process and Design ConsiderationsUntargeted analysis using liquid chromatography with tandem mass spectrometry (LC–MS/MS) chemical profiling is a valuable tool, but care needs to be taken with experimental design.
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Rapid Simultaneous Determination of Five Major Alkaloids from Menispermi Rhizoma in Rat Urine by Ultrahigh-Pressure Liquid Chromatography– Tandem Mass Spectrometry (UHPLC–MS/MS) and its Application to a Urinary Excretion StudyA UHPLC–MS/MS method is described for rapid quantification of five major bioactive alkaloids in rat urine. The results obtained help lay the foundation for the clinical application and safety evaluation of the bioactive ingredients of menispermi rhizoma, used in herbal medicines.
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GC Troubleshooting GuideMaster Gas Chromatography Troubleshooting - Streamline Your Analysis with Expert Guidance.
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Py-GC-MS Investigation of Pyrolysis Behaviours and Decomposition Products of α- and β-2,7,11-Cembratriene-4,6-DiolsThe study of the thermal behaviour and pyrolysis products of these terpenoids could possibly suggest flavour precursors that could be used to provide specific flavours to tobacco.
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Liquid Chromatography–Mass Spectrometry Analysis of Hop-Derived Humulone and Isohumulone Constituents in Beer: The Bitter Truth of Hops Utilization During BrewingA rapid LC–MS method using ESI coupled with SIM-MS for analysis of humulone and isohumulone content in beer is described.
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Essentials of LC Troubleshooting, Part II, Continued: More Misbehaving Retention TimesWhen can analyte retention deviate from what is expected or normal? We explain three subtle causes.
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Wastewater Surveillance of Pharmaceuticals and Genes: Challenges and OpportunitiesSample preparation and analysis of pharmaceuticals in wastewater present unique challenges. Here, we describe those challenges.
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Comparison of PFAS Sample Preparation Between WAX + Dispersive GCB and WAX/GCB Cartridges from Water and Soil Extracts Per Draft EPA 1633How to speed up sample prep time and equivalent acccuracy and precision.
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Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography MethodsA column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
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A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS TransmissionThe investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.
