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Novel 3D-Printing Method to Create Liquid Chromatography ColumnsThree-dimensional (3D) printing shows promise for creating stationary phases with tailored geometries. We present a method that overcomes limitations of other approaches, and can produce columns with a feature resolution of 10 μm.
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Aflatoxins Analysis in A Wide Range of Samples By LCDetect aflatoxins B1, B2, G1, and G2 and sterigmatocystin in complex (and simple) sample matrices using a streamlined approach with LC.
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Liquid Chromatographic Peak Purity Assessments in Forced Degradation Studies: An Industry PerspectiveThis review article discusses scientific rationales and current best practices in the pharmaceutical industry for performing chromatographic peak purity assessments (PPA). These activities are associated with the development and validation of liquid chromatographic (LC) stability-indicating analytical methods applicable to regulatory submissions of small-molecule drug candidates. The discussion includes a comprehensive overview of the PPA-related regulatory and scientific landscape and common industry approaches to obtain PPA results, as well as the strengths and weaknesses of PDA-facilitated ultraviolet (UV) PPA and other PPA techniques.
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Volatile Petrochemical Hydrocarbons AnalysisAnalysis of volatile petrochemical hydrocarbons in the environment by fully automated workflow (flow-modulated headspace–GC×GC–FID) for efficient sample quantitation
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Passing the Test: Understanding Proficiency TestingLaboratories use proficiency tests (PTs) to comply with their accreditation requirements and evaluate analysts’ performance. Laboratories regard PTs as a burdensome chore that must be successfully completed to satisfy internal or external compliance or accreditation requirements. PTs are an integral part of a quality management system (QMS) under quality assurance and control (QA/AC). Understanding the core components of the QMS is an important part of passing any PT test. Unacceptable PT results may have little to do with the result itself but reflect the use and application of statistics, standards, and methods.
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Postnova - Separation and Sizing of a Virus Mixture Using Asymmetrical Flow Field‐Flow Fractionation Coupled to Multi‐Angle Light ScatteringBiogen and Postnova present data on the analysis of adeno-associated viruses (AAV) using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering detection (AF4-MALS). AAV are promising gene therapy delivery vehicles, whose efficacy may be negatively affected by the presence of viral aggregates. Due to its gentle separation and broad applicable size range, AF4-MALS is able to characterize AAV and their aggregates with high resolution and precision, thereby overcoming the drawbacks that column-based chromatography techniques often face when dealing with samples larger than 50 nm in size.
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Metabolomics Meets Machine Learning: The Future of Nature-Inspired Drug DiscoveryResearchers at Enveda Biosciences are transforming nature into new medicines with their artificial intelligence (AI)-enabled industrial-scale drug discovery platform incorporating tandem mass spectrometry (MS/MS).
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Your 70:20:10 Development Plan for ChromatographersAspiring separation scientists should prepare for career development as well as they prepare their mobile phases. Can the 70:20:10 career development plan work for you?
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Accelerating Sample Preparation for the Analysis of Complex SamplesGiven that sample preparation consumes over two thirds of analysis time, sample preparation becomes the bottleneck issue in analytical chemistry, resulting in the urgent necessity of developing accelerated sample preparation techniques.
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An Assessment of Stationary Phase Selectivity in SFCSustainability concerns are renewing interest in SFC, often as a substitute for HPLC. With the broader application of SFC, we need a better understanding of selectivity in SFC, where stationary phase classification is not well established. This study assessed and measured the selectivity differences afforded by three prototype SFC phases.
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A Fast and Novel Chromatography-Free Forensics Toxicology Urine Screen With DART-MSMSWebinar Date/Time: Tue, Oct 24, 2023 11:00 AM EDT
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Intact Mass Analysis–Based Multi-Attribute Methods (iMAMs) for Characterization of BiopharmaceuticalsSome traditional MAM workflows are being substituted with iMAM workflows that rely on analyzing the intact mass of mAbs in their native form, resulting in significant improvements in high-throughput sample analysis.
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Gas Chromatography and Ion Mobility Spectrometry: A Perfect Match?The basic operating principles of ion mobility spectrometers (IMS), their resulting strengths and weaknesses, and why both perfectly align with the capabilities and requirements of gas chromatographs are discussed.
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The Importance of Sample Preparation in Quantitative Bioanalytical LC–MSA concise summary and celebration of the role of sample preparation for quantitative analysis in bioanalytical applications.
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Systematic Evaluation of HILIC Stationary Phases for MS Characterization of OligonucleotidesHydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.
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Size-Exclusion Chromatography–Mass Spectrometry Analysis of mAbs on a Biocompatible Flow PathThis article details a recent experiment that demonstrates how nonspecific interactions can be prevented by using metal-free or low adsorption flow paths.
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Method Development for the Qualitative and Quantitative Analysis of Pesticides by Direct Injection Liquid Chromatography– Tandem Mass Spectrometry (LC–MS/MS)This modification of ASTM method D8026 for pesticides in environmental matrices includes more pesticides and lowers the reporting limits, thus increasing throughput and measurement capacity for a large surface-water monitoring project.
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Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography MethodsA column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.
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Supercritical Fluid Chromatography Coupled with Drift Time Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry as a Tool for Lipid Characterization of HepG2 CellsLipids in SFC are mainly separated according to their lipid classes, which simplifies quantification with standards. In this study, SFC coupled with drift time IM‑QTOF-MS was used to characterize the HepG2 lipidome.
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Ion Chromatography Separation of Alkali, Alkaline Earth, Transition, Post-transition, Lanthanide, and Actinide Metal Cations in Waste SludgeThe authors developed an ion chromatography method to separate 31 cations in a single chromatographic run using inductively coupled plasma–mass spectrometry, liquid scintillation counting, and gamma counting. The high-level radioactive waste samples contained trace radionuclides in concentrations of only a few hundred disintegrations-per-minute-per-milliliter.
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Enter the Matrix: Improving the Interpretation of Separations Data Using Chemometrics in Analytical InvestigationsExamples from food science illustrate the usefulness of chemometrics techniques like pattern recognition, regression, and classification.
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Boosting the Purification Process of Biopharmaceuticals by Means of Continuous ChromatographySingle-column (batch) chromatography, involving two or more successive single-column (batch) chromatographic steps, is a standard approach for purifying biopharmaceuticals. Step one, known as the capture step, is used to remove product-related impurities, and step two, the polishing step, is used to remove product-related impurities. Here we present and illustrate the advantages of continuous chromatography for these separations: capture simulated moving bed (captureSMB) for the capture step and multicolumn countercurrent solvent gradient purification (MCSGP) for polishing.
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A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS TransmissionThe investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.
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Benefits of Integrating Travelling Wave Ion Mobility Spectrometry into Liquid Chromatography and Mass Spectrometry Workflows for Steroid AnalysisThe advantages provided by the implementation of ion mobility spectrometry (IMS), and in particular travelling wave ion mobility spectrometry (TWIMS), in traditional liquid chromatography–mass spectrometry (LC–MS) systems are discussed.
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Development of Gas Chromatography Instruments at ShimadzuThe guest authors summarize the history of this Japanese scientific instrument company and events in the development of its products.
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Enter the Matrix: Improving the Interpretation of Separations Data Using Chemometrics in Analytical InvestigationsExamples from food science illustrate the usefulness of chemometrics techniques like pattern recognition, regression, and classification.
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Portable and Field‑Deployable Liquid Chromatography for Environmental StudiesLiquid chromatography (LC) is a platform technology amenable to portable and “at-site” or deployable applications. This has awoken end-users to new possibilities and potential cost savings and process improvements.
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Assessing How Chemical Exposures Affect Human HealthMeasuring chemical exposure is extremely challenging due to the range and number of anthropogenic molecules encountered in our daily lives, as well as their complex transformations throughout the body. To broadly characterize how chemical exposures influence human health, a combination of genomic, transcriptomic, proteomic, endogenous metabolomic, and xenobiotic measurements must be performed. However, while genomic, transcriptomic, and proteomic analyses have rapidly progressed over the last two decades, advancements in instrumentation and computations for nontargeted xenobiotic and endogenous small molecule measurements are still greatly needed.
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Future-Proofing Validated Pharmacopeia Methods: A Case Study of Improving Throughput and Decreasing Operational CostHow to modernize an older pharmaceutical monograph from an HPLC column to an UHPLC column, along with calculated time and solvent savings.
