Author
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A Turnkey Protocol for Detecting Taste and Odor Compounds in Drinking and Surface Waters by Gas Chromatography–Mass Spectrometry and Electron Capture Detector (GC–MS/ECD)A comprehensive monitoring protocol has been developed using GC–MS/ECD in selective ion monitoring (SIM) mode, with injection performed by solid-phase microextraction (SPME) and headspace (HS). This single system has been configured to analyze for all taste and odor (T&O) compounds in Standard Method 2170, with minimal changing of columns, injectors, or SPME fibers between methods.
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Optimized Explosives Analysis Using Portable Gas Chromatography–Mass Spectrometry for Battlefield ForensicsWhen explosives are encountered on the battlefield, the use of portable GC–MS is valuable for the detection and confirmatory identification of pre- and post-detonation threats. In addition, this technique provides information about the source of explosives based on the detection and identification of trace-level chemicals in the sample. The data presented here confirm this capability.
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Untargeted Screening and Identification of Contaminants Using Gas Chromatography Coupled to High-Resolution Accurate Mass–Mass Spectrometry: Novel Solutions for Applied Testing LaboratoriesLimitations often arise when using GC with quadrupole-based mass spectrometers for detecting volatile and semivolatile contaminants. Enter HRAMS-MS.
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Free Excel Software for Performing Virtual Liquid ChromatographyA new free simulator is available for students, educators, and trainers to teach and perform virtual HPLC experiments that are applicable to real HPLC instrumentation and method development.
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The Role of mD-LC–MS for Antibody Analysis in the Biopharmaceutical IndustryHow is mD-LC–MS changing the way we evaluate HPLC peaks and assess performance?
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An Ultrahigh-Performance Liquid Chromatography Alternative to Animal Testing for Skin SensitizationOECD-compliant amino acid derivative assay (ADRA) testing and method transfer to UHPLC conditions is presented.
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Novel LC–MS/MS Method with a Dual ESI and APCI Ion Source for Analysis of California-Regulated Pesticides and Mycotoxins in Medium-Chain Triglyceride (MCT) Oil Cannabis TincturesA novel liquid chromatography–tandem mass spectrometry (LC–MS/MS) method with a dual electrospray ionization (ESI) and atmospheric-pressure chemical ionization (APCI) source was developed for analysis of 66 pesticides and 5 mycotoxins regulated by the State of California in medium-chain triglyceride (MCT) oil cannabis tinctures. The limits of quantitation (LOQs) of all of the pesticides and mycotoxins were well below California state action limits of these analytes in cannabis-based non-inhalable or edible products. A total of 62 (out of 66) pesticides and 5 mycotoxins were analyzed using LC–MS/MS with an ESI source, and the remaining 4 pesticides were determined using LC–MS/MS with an APCI source. A simple, fast, and cheap acetonitrile solvent extraction method was used for sample preparation to get good recovery and achieve high throughput for this analysis. For late-eluted analytes, a number of internal standards were used to compensate for ion suppression effects from the hydrophobic matrix.
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Acid-Catalyzed Isomerization of Carbonyls-2,4- dinitrophenylhydrazone in Mainstream Smoke of Heat-Not-Burn Tobacco Product for HPLC AnalysisErrors arising from the DNPH approach commonly used to analyze carbonyl compounds in smoke from heat-not-burn (HNB) tobacco can be avoided by using the approach described in this study.
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Ion-Exchange Displacement Chromatography: Scale-Up and Displacer Clearance for Recombinant Human Brain-Derived Neurotrophic FactorDisplacement chromatography is a viable alternative to elution chromatography, but the biotechnology industry has yet to fully exploit this technique. The authors present results of a pilot-scale ion-exchange displacement process developed for the removal of variants from recombitant human brain-derived neutrotrophic factor.
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Next Generation Peak Fitting for SeparationsSeparation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.
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But Why Doesn’t It Get Better? Kinetic Plots for Liquid Chromatography, Part 3: Pulling It All TogetherThe impact of extracolumn dispersion on kinetic plot curves is investigated. A web-based calculator that allows users to make their own kinetic plots with examples of how the tool can be used to troubleshoot underperforming columns and support column selection is discussed.
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The Power of Trypsin Immobilized Enzyme Reactors (IMERs) Deployed in Online MDLC–MS ApplicationsCommercially available trypsin IMERs can digest proteins with high sequence coverage and robustness, facilitating online multidimensional LC–MS.
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Efficient Sample Preparation and High-Capacity Columns for Successful Food and Beverage AnalysisOvercoming the challenges of food and beverage analysis with ion chromatography (IC).
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Extractable and Leachable Testing for Pharmaceutical Packaging, Finished Pharmaceutical Products, and Medical Devices: An Analytical PerspectiveExtractable and leachable (E&L) testing for finished pharmaceutical products, bioprocess manufacturing systems, and medical devices is currently a subject of intense interest. The majority of the challenges encountered in analytical workflows are related to the highly complex matrices and relatively low analyte evaluation thresholds seen in E&L studies. This paper provide options for executing E&L testing to supporting regulatory submissions. There are multiple compliant routes possible, and the presented workflows and analytical solutions are only one of multiple successful approaches.
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Quantitative Analysis of PFAS in Milk, Infant Formula, and Related Ingredients Using LC–MSThis method for PFAS analysis in milk and infant formula is robust, reliable, and reproducible, with scope to expand the list of PFAS in the future.
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Fully Automated QuEChERS Extraction and Cleanup of Organophosphate Pesticides in Orange JuiceA fully automated quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and extract clean-up method is described for analysis of organophosphate pesticides in orange juice. This method uses GC–MS and LC–MS along with an industry standard robotic x,y,z-sampling system.
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Solving Key Challenges in (Bio)pharmaceutical AnalysesThis article discusses several practical examples using bioinert columns, demonstrating their ability to deliver reliable, high-resolution results with precision and reproducibility.
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Sample Preparation by Electric Field–Assisted ExtractionIntegrating electric fields into extraction techniques increases sensitivity and selectivity. These approaches are compatible with miniaturized, portable, and many biological samples.
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Monoliths as Stationary Phases for Separating Biopolymers – Fourth-Generation Chromatography SorbentsMonoliths are chromatography sorbents cast into columns as a single continuous piece in contrast with regular chromatographic sorbents, which are packed as individual particles. The guest authors compare three such novel sorbents with a conventional particle-packed column.
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The Role of mD-LC–MS for Antibody Analysis in the Biopharmaceutical IndustryHow is mD-LC–MS changing the way we evaluate HPLC peaks and assess performance?
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The State of the Art in Life Science Analysis; Virtual SymposiumWebinar Date/Time: Wednesday, October 18th, 2023 12:30 pm BST 7:30 am EDT 13:30 pm CEST Thursday, October 19th, 2023 12:30pm BST | 7:30am EDT | 13:30pm CEST
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Reducing Sample Size and Obtaining Representative SamplesGuest author Reg Cross discusses strategies for collecting samples from large masses of material. He also describes the problems associated with acquiring truly representative samples for analysis.
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Optimization of Determination Method of Cooling Agents in Cigarette Tipping Paper by Gas ChromatographyThis GC–FID method enables accurate determination of cooling agents, which are flavor compounds commonly used in cigarette tipping paper.
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Characterization of Styrene–Butadiene Block Copolymers by Gel Permeation ChromatographyThe authors compare their results in analyzing styrene–butadiene block copolymers by gel permeation chromatography with other methods, such as Fourier transform infrared spectroscopy and pyrolysis gas chromatography.
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Retaining Talent in Field-Flow Fractionation: An InitiativeThe authors present their motivation for establishing the Young Scientists of FFF (YSFFF) initiative within the FFF community.
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The Ever-Changing World of Extractables and Leachables: Lessons learned and future challengesOctober 4, 2021 at 3pm BST | 10am EST | 4pm CEST Join global industry and regulatory experts as they discuss current hot topics in extractables and leachables (E&L) testing for pharmaceuticals, biopharmaceuticals and medical devices.
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Six Key Differentiators Between Liquid Chromatography and High-Resolution Ion MobilityUsing ion mobility, analytes that have the same molecular mass can be separated by their shape, centers of mass, and collision cross section, but challenges such as ion loss can still occur. A new development in ion mobility separation, high-resolution ion mobility (HRIM), addresses such problems, and is particularly well suited to challenging applications, such as glycosylation monitoring of biological drugs and vitamin D analysis.
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Dispersive Solid-Phase Extraction and Solid-Phase Extraction for ppt-level PFAS Analysis in Apples: A ComparisonPer- and polyfluoralkyl substances (PFAS) are found in our food. Sensitive, precise, and accurate analytical methods are needed to estimate human exposure to these chemicals. A comparative study was performed between two extraction and cleanup methods (solid-phase extraction [SPE] and dispersive SPE) for the analysis of PFAS in apples. Both methods showed excellent sensitivity, precision, and accuracy. dSPE has some benefits over conventional SPE, and vice versa. The advantages and disadvantages of both methods are discussed.
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Systematic Evaluation of HILIC Stationary Phases for MS Characterization of OligonucleotidesHydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.
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Retaining Talent in Field-Flow Fractionation: An InitiativeThe authors present their motivation for establishing the Young Scientists of FFF (YSFFF) initiative within the FFF community.
