A review article that summarizes the successful sample preparation strategies that have led to some of the highest peptide and protein identification rates reported in the literature
The need to increase the use of low valued co-products derived from the processing of sugar beets has prompted the investigation of the structure of the pectin extracted from sugar beet pulp. The characterization of sugar beet pectin is essential as it has the potential to be used in the production of industrial products, e.g., as an emulsifying agent in food systems.
A carefully chosen analytical technique can enhance the inherent advantages of passive sampling of watercourses over more traditional "grab" or "spot" sampling.
Microwave-accelerated extraction (MAE) is described and evaluated. The latest enhancements to this technology are discussed from a hardware and applications perspective.
LCGC NA - The Quantification of Clopidogrel in Human Plasma
The development of various analytical MS methods to investigate the chemical composition of liquids used in electronic cigarettes and characterize their quality is presented in this study.
An overview of different approaches for the qualitative and quantitative analysis of polysorbates.
The variation in selectivity of aromatic hydrocarbons with bisubstituted polar groups is investigated in systems consisting of C18 stationary phase and ternary eluents. The solutions of the ternary eluents were obtained by mixing binary solvents (organic modifier + water) of similar eluent strength. Acetonitrile, methanol and tetrahydrofuran were the organic modifiers applied. The influence of the organic solvent type and its concentration in the ternary mobile phase on retention and selectivity of the solutes is discussed. An approach previously presented by our group was adapted to explain the selectivity changes.
A systematic approach for formation of sigmoidal gradient for analysis of biopharmaceutical proteins has been reviewed and discussed together with two case studies. The first application involves HPLC separation of the various product related variants of a microbial expressed biotherapeutic, granulocyte colony stimulating factor (GCSF). The second case study involves separation of the charge heterogeneity related variants for a monoclonal antibody (mAb) biotherapeutic product. In all cases, it is observed that the use of sigmoidal gradient successfully reduces the analysis time significantly (from 70 minutes to 15 minutes for GCSF and from 40 minutes to 4 minutes for the mAb) while retaining the selectivity and the resolution.
The development of various analytical MS methods to investigate the chemical composition of liquids used in electronic cigarettes and characterize their quality is presented in this study.
Simple, rapid and accurate methods have been developed for the analysis of simple carbohydrates including mono- or disaccharides, or as fairly small glycans (6–11 residues) liberated from glycoproteins using charged aerosol detection combined with mass spectrometry techniques.
Insulin was the first recombinant pharmaceutical produced. Currently, one of the critical quality control attributes of therapeutic insulin in the US and European Pharmacopoeia (USP and EP) monograph is the level of covalent high molecular weight (HMW) insulin as determined by HPLC size-exclusion chromatography.
The use of electrospray ionization–mass spectrometry for rapid and specific determination of the widely used metal deactivators Irgamet-30 and Irgamet-39 is demonstrated here.
The FDA has made changes to how products containing sunscreen are labeled in the U.S. to ensure they meet the new regulations set forth for safety and effectiveness. The new regulations will require companies that want to use the "broad spectrum" label to test for both UVA and UVB protection.
This article compares results from samples prepared and analyzed according to EPA Method 1613B on a sector instrument with those from a high-resolution, accurate mass TOF mass spectrometer and a low-resolution TOF mass spectrometer interfaced to a comprehensive two-dimensional gas chromatography (GC) system (GC?C–TOF-MS).
An application of MALDI-MS in qualitative and quantitative analysis of pharmaceutical compounds spiked in urine is demonstrated.
Advantages of the planar, off-line format of TLC relative to on-line column HPLC, the steps in TLC analyses, and future prospects of TLC are discussed.
Webinar Date/Time: Thu, Sep 5, 2024 11:00 AM EDT
Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
This work describes a simple and sensitive high performance liquid chromatography (HPLC) method with mass spectrometry (MS) and ultraviolet (UV) detection for the analysis of mixtures of up to 32 pesticides.
Analysis of chromium species is made challenging due to the nature of the element and diverse sample matrices
A novel "green chromatography" method for analysing melamine in milk is described. The method uses 100% water as the eluent and in the sample preparation stage and is reported to be a reliable and cost-effective method for laboratories in developing countries.
Monoclonal antibodies (mAbs) are being developed at an explosive rate and have attracted great interest from both smaller biotech firms and big pharmaceutical companies. Developing mAbs and next-generation antibody–drug conjugates (ADCs) is highly demanding in many ways. From an analytical perspective, handling mAbs and ADCs presents many new challenges. This article describes how size-exclusion chromatography (SEC) combined with high-resolution mass spectrometry (HRMS) can be applied to the detailed characterization of mAbs and ADCs.
A look at the LC and LC–MS methods that are helping to overcome challenges in sample analysis, and how this can make adeno‑associated virus (AAV)–based gene therapy more accessible.
In on-site environmental applications, representative sampling and proper replication are essential. For these reasons, recent work in thin-film solidphase microextraction has focused on the development of unique holders and customized samplers that are tailored for distinct sampling environments. Here, we explore the latest developments.
This article describes the analysis of fluorescently labelled N-glycans released from proteins by liquid chromatography–mass spectrometry (LC–MS).
HPLC with diode-array and electrochemical-array detection overcomes sensitivity and specificity limitations in natural products analysis.