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Sigmoidal Gradients in the HPLC-Based Analysis of Biotherapeutic ProductsA systematic approach for formation of sigmoidal gradient for analysis of biopharmaceutical proteins has been reviewed and discussed together with two case studies. The first application involves HPLC separation of the various product related variants of a microbial expressed biotherapeutic, granulocyte colony stimulating factor (GCSF). The second case study involves separation of the charge heterogeneity related variants for a monoclonal antibody (mAb) biotherapeutic product. In all cases, it is observed that the use of sigmoidal gradient successfully reduces the analysis time significantly (from 70 minutes to 15 minutes for GCSF and from 40 minutes to 4 minutes for the mAb) while retaining the selectivity and the resolution.
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Analysis of Nicotine Alkaloids and Impurities in Liquids for e-Cigarettes by LC–MS, GC–MS, and ICP-MSThe development of various analytical MS methods to investigate the chemical composition of liquids used in electronic cigarettes and characterize their quality is presented in this study.
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Glycan Analysis using HPLC with Charged Aerosol Detection and MS DetectorsSimple, rapid and accurate methods have been developed for the analysis of simple carbohydrates including mono- or disaccharides, or as fairly small glycans (6–11 residues) liberated from glycoproteins using charged aerosol detection combined with mass spectrometry techniques.
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Size-Exclusion Ultra Performance Liquid Chromatography Analysis of InsulinInsulin was the first recombinant pharmaceutical produced. Currently, one of the critical quality control attributes of therapeutic insulin in the US and European Pharmacopoeia (USP and EP) monograph is the level of covalent high molecular weight (HMW) insulin as determined by HPLC size-exclusion chromatography.
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Rapid and Specific Determination of Contaminants, By-Products, and Additives in Insulating Mineral Oils with Tandem Mass SpectrometryThe use of electrospray ionization–mass spectrometry for rapid and specific determination of the widely used metal deactivators Irgamet-30 and Irgamet-39 is demonstrated here.
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Analysis of "Broad Spectrum" UVA and UVB Components in Sun Care Products for Compliance with New FDA RegulationsThe FDA has made changes to how products containing sunscreen are labeled in the U.S. to ensure they meet the new regulations set forth for safety and effectiveness. The new regulations will require companies that want to use the "broad spectrum" label to test for both UVA and UVB protection.
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Applying Comprehensive Analysis to EPA Method 1613B Samples — Discover Those Compounds Usually Discounted in Environmental SamplesThis article compares results from samples prepared and analyzed according to EPA Method 1613B on a sector instrument with those from a high-resolution, accurate mass TOF mass spectrometer and a low-resolution TOF mass spectrometer interfaced to a comprehensive two-dimensional gas chromatography (GC) system (GC?C–TOF-MS).
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Rapid and Direct Quantitation of Pharmaceutical Drugs from Urine Using MALDI-MSAn application of MALDI-MS in qualitative and quantitative analysis of pharmaceutical compounds spiked in urine is demonstrated.
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Stationary Phases for Modern Thin-Layer ChromatographyAdvantages of the planar, off-line format of TLC relative to on-line column HPLC, the steps in TLC analyses, and future prospects of TLC are discussed.
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Unveiling CQA Insights for mRNA, LNPs, and Viral Vectors by SEC and MALS With Novel 1000 Å Ultrawide Pore Particle ColumnsWebinar Date/Time: Thu, Sep 5, 2024 11:00 AM EDT
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Enhancing Peptide Mapping Sequence Coverage Through an Automated Dual Protease DigestTrypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.
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Qualitative LC–MS Analysis of Pesticides Using Monolithic Silica Capillaries and Potential for Assay of Pesticides in KidneyThis work describes a simple and sensitive high performance liquid chromatography (HPLC) method with mass spectrometry (MS) and ultraviolet (UV) detection for the analysis of mixtures of up to 32 pesticides.
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Hexavalent Chromium Determination HamiltonAnalysis of chromium species is made challenging due to the nature of the element and diverse sample matrices
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"Green" HPLC with 100% Water Eluent for Analysing Melamine in MilkA novel "green chromatography" method for analysing melamine in milk is described. The method uses 100% water as the eluent and in the sample preparation stage and is reported to be a reliable and cost-effective method for laboratories in developing countries.
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Denaturing and Native Size-Exclusion Chromatography Coupled to High-Resolution Mass Spectrometry for Detailed Characterization of Monoclonal Antibodies and Antibody–Drug ConjugatesMonoclonal antibodies (mAbs) are being developed at an explosive rate and have attracted great interest from both smaller biotech firms and big pharmaceutical companies. Developing mAbs and next-generation antibody–drug conjugates (ADCs) is highly demanding in many ways. From an analytical perspective, handling mAbs and ADCs presents many new challenges. This article describes how size-exclusion chromatography (SEC) combined with high-resolution mass spectrometry (HRMS) can be applied to the detailed characterization of mAbs and ADCs.
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Applying LC and LC–MS Methods to the Characterization and Analysis of rAAVA look at the LC and LC–MS methods that are helping to overcome challenges in sample analysis, and how this can make adeno‑associated virus (AAV)–based gene therapy more accessible.
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Frontiers of Sampling: Design of High Surface Area Thin-Film Samplers for On-site Environmental AnalysisIn on-site environmental applications, representative sampling and proper replication are essential. For these reasons, recent work in thin-film solidphase microextraction has focused on the development of unique holders and customized samplers that are tailored for distinct sampling environments. Here, we explore the latest developments.
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A Mixed-Mode Stationary Phase for the Structural Analysis of Labelled N-Glycans Using LC–MS–MSThis article describes the analysis of fluorescently labelled N-glycans released from proteins by liquid chromatography–mass spectrometry (LC–MS).
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The Spectro-Electro Array: A Novel Platform for the Measurement of Secondary Metabolites in Botanicals, Supplements, and BeveragesHPLC with diode-array and electrochemical-array detection overcomes sensitivity and specificity limitations in natural products analysis.
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Low Level PAH Detection with an Extensive Dynamic RangeThe analysis of PAHs in less than 16 min using hydrogen as carrier gas and the PerkinElmer? Clarus? SQ 8 GC/MS operating in SIM mode is presented. Detection limits down to 0.5 pg on-column and a wide dynamic range are reported.
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Screening by LC–MS-MS and Fast Chromatography: An Alternative Approach to Traditional Immunoassays?A new technique was developed based on liquid chromatography coupled with tandem mass spectrometry to analyze drugs that are not part of current immunoassay screening, specifically zolpidem, zopiclone, and zaleplone, or "z-drugs."
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Recent Advances in Pressurized-Fluid ExtractionCurrent advances in commercial pressurized-fluid extraction (PFE) instrumentation are discussed, along with the advantages of performing PFE prior to chromatographic analysis.
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Probing Aqueous Surfaces by TOF-SIMSA breakthrough using a microfluidic interface to conduct sensitive time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis and study liquid surfaces in situ under vacuum conditions is described here.
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Effective Cleanup and Analysis of Metronidazole from Foaming Facial CleanserWhen working with complex matrices such as personal care products, sample preparation is perhaps the most difficult step of the analysis process. By utilizing a targeted sample preparation technique, such as ion-exchange SPE, analysis can be significantly improved as compared to less targeted techniques such as liquid-liquid extraction.
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Ultratrace Quantitive Analaysis of Catalyst Poisoners Using a Dedicated GC–MS AnalyserA dedicated GC– MS analyser was developed to address the increasing need for more sensitive catalyst poisoner analysis. The system combines the separation power and robustness of a classic backflush configuration with the selectivity and sensisitivitiy of mass spectrometry.
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Fast Cap IC Determinations of Inorganic Anions and Cations in Drinking WaterThermo Scientific Application Note
