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Determination of 2-Hydroxy-4-(methylthio)butanoic Acid in Bovine Serum and Sea Water

Steve Lobert;Racha Seemamahannop;Balaji Viswanathan;Shubhen Kapila
July 1st 2014

Simple, sensitive, rapid, selective, and precise reversed-phase liquid chromatography (LC), electrospray ionization mass spectrometry (ESI-MS), and tandem MS (ESI-MS-MS) methods were developed and validated for the determination of 2-hydroxy-4-(methylthio)-butanoic acid (HMTBA) in bovine serum and sea water matrix. HMTBA is the ?-hydroxy analog of the sulfur-containing amino acid methionine and is extensively used as a methionine supplement in poultry and bovine feed.

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