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Modernization of the Acetaminophen USP Monograph Gradient HPLC Method for Impurities using USP <621> Guidelines and MaxPeak Premier HPS TechnologyThis application note examines the USP acetaminophen impurities using an HPLC system and UPLC System. Improvements in sample throughput and solvent usage were realized by using newer technology.
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Optimizing Flavour Analysis Using Modern Heart-Cutting MDGCThis review discusses recent technological advances in classical heart-cuttting two-dimensional gas chromatography (GC–GC). These developments are then illustrated by application to analysis of important flavour compounds at trace levels in very complex matrices
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The Characterization of Sugar Beet Pectin using the EcoSEC GPC System Coupled to Multi-Angle Light Scattering, Quasi-Elastic Light Scattering, and Differential ViscometryThe need to increase the use of low valued co-products derived from the processing of sugar beets has prompted the investigation of the structure of the pectin extracted from sugar beet pulp. The characterization of sugar beet pectin is essential as it has the potential to be used in the production of industrial products, e.g., as an emulsifying agent in food systems.
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Cover Story - Technical Challenges Encountered During Chromatographic Method Transfers to Pharmaceutical Manufacturing SitesDrug substance development requires a range of analytical methods to be developed to generate process knowledge and to support in-process and release testing throughout a synthetic sequence. This article describes practical examples of a wide variety of transfer challenges and our remediation strategy.
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Petrochemical ProfilingThe Column spoke to Hans van den Heuvel, the Product Marketing Manager for Bruker Chemical & Applied Markets.
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Sample Preparation for Chromatography: How Much Can Be Automated?The author reviews the automation capabilities available, and some practical considerations to take into account when choosing to automate some or all of the sample preparation and handling steps that must be done before analysis.
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Lenses or No Lenses? A Study of Ion Transfer Efficiency at Interfaces in a Lens-Free Triple Quadrupole MSThe efficiency of ion transfer between the quadrupole mass analyzers and the Q2 collision cell in a triple quadrupole mass spectrometry (MS) is critical for instrument robustness and sensitivity. Q2 is a radio frequency (RF) only non-mass filtering quadrupole containing only an inert collision gas such as Ar, He, or N2 to provide collision induced dissociation of the precursor ion selected in Q1.
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Retention and Selectivity of Aromatic Hydrocarbons with Polar Groups in Ternary Reversed-Phase–HPLC Systems with Tetrahydrofuran as ModifierThe variation in selectivity of aromatic hydrocarbons with bisubstituted polar groups is investigated in systems consisting of C18 stationary phase and ternary eluents. The solutions of the ternary eluents were obtained by mixing binary solvents (organic modifier + water) of similar eluent strength. Acetonitrile, methanol and tetrahydrofuran were the organic modifiers applied. The influence of the organic solvent type and its concentration in the ternary mobile phase on retention and selectivity of the solutes is discussed. An approach previously presented by our group was adapted to explain the selectivity changes.
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Rapid and Specific Determination of Contaminants, By-Products, and Additives in Insulating Mineral Oils with Tandem Mass SpectrometryThe use of electrospray ionization–mass spectrometry for rapid and specific determination of the widely used metal deactivators Irgamet-30 and Irgamet-39 is demonstrated here.
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Microwave-Accelerated Extraction — SW-846 Method 3546 and BeyondMicrowave-accelerated extraction (MAE) is described and evaluated. The latest enhancements to this technology are discussed from a hardware and applications perspective.
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Supercritical Fluid Chromatography for Chiral Analysis, Part 2: ApplicationsIn the second part of this review article, the recent progress in SFC for enantiomeric separations is evaluated. Several applications reported on the enantioselective separation of drugs and pharmaceutical compounds using chiral SFC are discussed, including pharmaceutical applications, clinical research, forensic toxicology, and environmental sciences.
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Tracing PAH Analysis with an Automated Sample Pretreatment Online SPE MethodShimadzu Application Note
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Control of Matrix Effects in Bioanalytical MS–MS Using On-line Multidimensional Solid-Phase ExtractionThe authors discuss a solid-phase extraction (SPE) clean-up procedure for drugs present in biofluids.
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Characterization of IgG monomers & their aggregatesTwo antibody samples were characterized using column calibration and multi-detection SEC, and the results compared to show how multi-detection SEC provides more accurate data and a more complete characterization of the protein mixtures under study.
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Investigating the Environmental Impact of OrganotinsExploring the concerns surrounding the use of organotin compounds and their impact on the environment and potential toxicity to mammals.
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Creating a High-Throughput LC–MS-MS System Using Common ComponentsHow to create a liquid chromatography–tandem mass spectrometry (LC–MS-MS) system using mass spectrometers, a high performance liquid chromatography (HPLC) binary pump system, and an autosampler
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Native Separation-Mass Spectrometry in Biopharmaceutical AnalysisInnovative native separation strategies coupled with native mass spectrometry techniques to characterize biopharmaceuticals close to their natural state are described.
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Field-Flow Fractionation for Biological, Natural, and Synthetic Polymers: Recent Advances and TrendsA review of the latest trends in field-flow fractionation (FFF) for various types of polymer analysis.
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Detecting Environmental PFAS Using Liquid Chromatography–Tandem Mass SpectrometryThe presence of per- and polyfluoroalkyl substances (PFAS) in products used every day by millions of people is a cause of concern among both consumers and scientists. PFAS found in drinking water and the environment can cause serious health issues in animals and humans. Amanda Belunis, who is a PhD candidate at the University of Maryland in Baltimore County, has been investigating the use of liquid chromatography–tandem mass spectrometry (LC–MS/MS) to detect PFAS from a variety of environmental sources. She spoke with us about methods used to detect PFAS and described a new method that she and her team have developed to enhance PFAS detection.
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Massively Parallel Phase Analysis Light Scattering Enables Reliable Mobility Measurements of Proteins, Nanoparticles and ColloidsTraditionally, measuring the mobility of proteins and nanoparticles has been a challenging task. An effective measurement method traditionally used is phase analysis light scattering (PALS), a technique based on interfering laser light scattered from a sample solution. However, it has been associated with two important shortcomings. Massively Parallel Phase Analysis Light Scattering (MP-PALS) has emerged as a viable alternative to the conventional PALS method.
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Creating a High-Throughput LC–MS-MS System Using Common ComponentsHow to create a liquid chromatography–tandem mass spectrometry (LC–MS-MS) system using mass spectrometers, a high performance liquid chromatography (HPLC) binary pump system, and an autosampler
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Efficient Quantitation of Biotherapeutics in Biological Matrices Through the Application of LC–MS‑Related TechniquesBiotherapeutics must endure in-depth testing to validate their efficacy and safety before their release to the medical community. Characterization and quantitation of these large molecule medicines is traditionally performed with ligand binding assays or radiolabeling procedures. Issues with selectivity, accuracy, and unavailability of applicable assays for the characterization and quantitation of certain biotherapeutics means that liquid chromatography–mass spectrometry (LC–MS) is becoming an increasingly selected method for biotherapeutics testing. Typically used for small molecules, LC–MS can be adapted for larger molecule analysis with additional high throughput and multiplexing capabilities. New method development has turned LC–MS into a highly sensitive option for biotherapeutics validation.
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Significant Improvements in Pesticide Residue Analysis in Food Using the QuEChERS MethodThe analysis of pesticide residues in food samples from the state of Connecticut's regulatory monitoring program are compared to USDA and US FDA results.
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UHPLC-UV Method for the Determination of Esomeprazole Using a Syncronis C18 1.7 ?m ColumnThis application note demonstrates the use of the Thermo Scientific Syncronis C18 1.7 ?m column for the determination of esomeprazole by UHPLC-UV.
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The Benefits of Using a Method Life Cycle Approach to Improve Method QualityThe Column spoke to Evelien Dejaegere from Waters Corporation about the method life cycle management approach and its importance in the pharmaceutical sector.
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A Simple and Effective ICP-MS Method Capable of Detecting Trace and Major Elements in Milk SamplesThe method described here is a simple method for detection of a wide spectrum of metals and for quantitative analysis over a wide dynamic range of elements, including both toxic metals and those metals essential for health and well-being.
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The Application of Capillary Electrophoresis for Vaccine Release and CharacterizationThrough a Q & A format, this article illustrates some of the uses of CE in this area.
