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Data Acquisition, Handling, and Archiving
Data Analysis, Statistics, and Chemometrics
Dietary Supplements Analysis
Environmental Analysis
Field-Flow Fractionation (FFF)
Food and Beverage Analysis
Forensics, Narcotics
From the Editor
GC–MS
Gas Chromatography (GC)
HILIC
HPLC
Ion Chromatography
LCGC Interviews
LCGC TV: Gas Chromatography
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Process Analytical Technology (PAT)
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Sample Preparation
Size-Exclusion Chromatography (SEC)
Solid-Phase Extraction (SPE)
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Supercritical Fluid Extraction (SFC)
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Lee J. Bertram

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Lee J. Bertram is with Agilent Technologies.

Articles

Microscopic illustration of DNA double helix structure, highlighting gene silencing techniques with RNA interference, CRISPR-Cas9, and antisense oligonucleotides, for genetic research applications. | Image Credit: © john - stock.adobe.com

Systematic Evaluation of HILIC Stationary Phases for MS Characterization of Oligonucleotides

Jordy J. Hsiao;Lee J. Bertram;Alex Apffel;Andrea Angelo P. Tripodi;Andrew Coffey;Ta-Chen Wei;Connor Flannery
October 15th 2024

Hydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.

Latest Updated Articles

Analyzing Organic Acids in Fruit-Based Kombucha Analogues Using HPLC
Published: November 15th 2024 | Updated: November 19th 2024
Analyzing Organic Acids in Fruit-Based Kombucha Analogues Using HPLC
LC-HRMS Technology Used to Track Chemical Migration in Reusable Plastic Bottles
Published: November 18th 2024 | Updated: November 19th 2024
LC-HRMS Technology Used to Track Chemical Migration in Reusable Plastic Bottles
Technology Trends in Separation Science: Data Handling
Published: July 3rd 2024 | Updated: July 8th 2024
Technology Trends in Separation Science: Data Handling
Published: December 19th 2024 | Updated: December 22nd 2024
Advances in Biopharmaceutical Analysis (Dec 2024)
Published: December 19th 2024 | Updated: December 22nd 2024
Advances in Biopharmaceutical Analysis EU
Vol 20 No 12 The Column December 2024 North America PDF
Published: December 4th 2024 | Updated: December 22nd 2024
Vol 20 No 12 The Column December 2024 North America PDF
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