Author


Christian Huber

Latest:

Higher Order Mass Spectrometry Techniques Applied to Biopharmaceuticals

Higher Order Mass Spectrometry Techniques Applied to Biopharmaceuticals


Landon Wiest

Latest:

When Do We Need Sub2m Superficially Porous Particles for Liquid Chromatography Separations

The use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.


Dan Li

Latest:

The Determination of Trace Per- and Polyfluoroalkyl Substances and Their Precursors Migrated into Food Simulants from Food Contact Materials by Liquid Chromatography–MS/MS and Gas Chromatography–MS/MS

An increasing range of per- and polyfluoroalkyl substances (PFAS) has been found migrating from food contact material into food. This article establishes an integrated analytical approach combining HPLC–MS/MS and GC–MS/MS to detect 36 such PFAS.


Verónica Pino

Latest:

Dispersive Miniaturized Solid‑Phase Extraction Using the CIM‑81 Metal‑Organic Framework and Gas Chromatography–Mass Spectrometry to Determine Personal Care Products in Waters

The analysis of contaminants found in environmental waters and originating from personal care products using metalorganic frameworks (MOFs) combined with liquid chromatography (LC) is described. This work expands the use of MOFs from gas chromatography to LC and also meets the requirements of green analytical chemistry.


Catalina Ruiz-Pérez

Latest:

Determination of Polycyclic Aromatic Hydrocarbons in Environmental Waters Using Heterogeneous Magnetic Materials Based on Metal–Organic Frameworks

This study of PAHs makes it easy to compare this microextraction procedure to conventional approaches and to other procedures using magnetic composites.


Priscilla Rocío-Bautista

Latest:

Determination of Polycyclic Aromatic Hydrocarbons in Environmental Waters Using Heterogeneous Magnetic Materials Based on Metal–Organic Frameworks

This study of PAHs makes it easy to compare this microextraction procedure to conventional approaches and to other procedures using magnetic composites.


Jorge Pasán, Juan H. Ayala

Latest:

Dispersive Miniaturized Solid-Phase Extraction Using the CIM-81 Metal-Organic Framework and Gas Chromatography–Mass Spectrometry to Determine Personal Care Products in Waters

In this study, the analysis of contaminants found in environmental waters and originating from personal care products is addressed using metalorganic frameworks (MOFs) in combination with liquid chromatography (LC). This work expands the use of MOFs from gas chromatography to LC and also meets the requirements of green analytical chemistry.


Ana M. Afonso

Latest:

Dispersive Miniaturized Solid‑Phase Extraction Using the CIM‑81 Metal‑Organic Framework and Gas Chromatography–Mass Spectrometry to Determine Personal Care Products in Waters

The analysis of contaminants found in environmental waters and originating from personal care products using metalorganic frameworks (MOFs) combined with liquid chromatography (LC) is described. This work expands the use of MOFs from gas chromatography to LC and also meets the requirements of green analytical chemistry.


LCGC’s CHROMacademy

Latest:

Graduate to Analyst – What it Takes

Webinar Date/Time: Thu, Jan 19, 2023 11:00 AM EST


Hartmut Frank

Latest:

Marjorie Groothuis Horning: Biochemist, Pharmacologist, and GC Pioneer

A look back at this innovator’s career and her efforts to develop and popularize gas chromatography (GC), particularly for biomedical research.


John Chasse

Latest:

Equipment Roundup: New Products Announced from SCIEX, Fraunhofer Institute for Integrated Circuits

In this “Equipment Roundup,” the editors of LCGC International present SCIEX’s ZenoTOF 7600+ System and Fraunhofer Institute for Integrated Circuits’ nanoSPECTRAL chip.


Jingshu Guo

Latest:

DNA Adductomic Analysis by Data-Independent Mass Spectrometry

A new type of analysis called “wide-selected ion monitoring (SIM)/MS2” scanning, is capable of screening for a wide range of DNA adducts (chemical modifications to genomic DNA). This method has successfully identified DNA adducts from carcinogen exposures and oxidative stress in human prostate and kidney tissues.


Peter W. Villalta

Latest:

DNA Adductomic Analysis by Data-Independent Mass Spectrometry

A new type of analysis called “wide-selected ion monitoring (SIM)/MS2” scanning, is capable of screening for a wide range of DNA adducts (chemical modifications to genomic DNA). This method has successfully identified DNA adducts from carcinogen exposures and oxidative stress in human prostate and kidney tissues.


Robert J. Turesky

Latest:

DNA Adductomic Analysis by Data-Independent Mass Spectrometry

A new type of analysis called “wide-selected ion monitoring (SIM)/MS2” scanning, is capable of screening for a wide range of DNA adducts (chemical modifications to genomic DNA). This method has successfully identified DNA adducts from carcinogen exposures and oxidative stress in human prostate and kidney tissues.


Anastasia Kalli

Latest:

Targeted Metabolomics Analysis of Type 2 Diabetes Serum Samples Using Mass Spectrometry and a Standardized Quantitative Method

An important advantage of standardized methods is that they enable comparability between laboratories and across studies. In this work, the author used a standardized targeted kit to demonstrate the accuracy, sensitivity, and reproducibility of the approach, analyzing serum samples obtained from type 2 diabetes study subjects and healthy controls.


Linx Waclaski

Latest:

Optimizing Splitless GC Injections

Splitless injections are sometimes necessary for trace analyses, where the analyst hopes to recover 100% of the analytes that are injected. Unfortunately, splitless injections can be challenging and using an imperfect method can lead to loss of analytes and poor peak shapes. The choice of inlet liner can have an impact on the data and one must consider the effects of geometry, packing, deactivation, and volume on introduction of analytes into the system. Other important inlet parameters to consider include inlet temperature, splitless hold time, and initial oven temperature.


Vaughan S. Langford

Latest:

Rapid Quantitative Analysis of Ethylene Oxide and 1,4-Dioxane in Polymeric Excipients Using SIFT-MS

Pharmaceutical excipients, such as polyethylene glycol-based polymers, must be tested for the presence of ethylene oxide (EtO) and 1,4-dioxane as part of a safety assessment, according to USP Chapter <228>.


Murray J. McEwan

Latest:

Rapid Classification of Beef Aroma Quality Using SIFT-MS

Application of multivariate statistical analysis to a combined data set demonstrated that SIFT-MS discriminates premium quality beef from eight sensory defects, and, therefore, could be applied as an instrumental grading tool, obviating sensory panel grading.


Mark J. Perkins

Latest:

Rapid Quantitative Analysis of Ethylene Oxide and 1,4-Dioxane in Polymeric Excipients Using SIFT-MS

Pharmaceutical excipients, such as polyethylene glycol-based polymers, must be tested for the presence of ethylene oxide (EtO) and 1,4-dioxane as part of a safety assessment, according to USP Chapter <228>.


Bob Clifford

Latest:

Fast and Simple High Performance Liquid Chromatography–Ultraviolet Assay for the Determination of Cannabinoid Content in Hemp Oil

The medicinal qualities of cannabinoids contained in hemp have been described in detail. Pain mitigation and reduced severity of nausea and seizures are just a few of the therapeutic benefits reported by medical cannabis patients. There is evidence that a combination of CBD, a host of other minor cannabinoids, and a complex array of terpenoids as contained in hemp oil may be the most beneficial, which is why CBD-rich oil for oral intake has become increasingly popular. The FDA has issued warning letters to firms that market unapproved new drugs allegedly containing CBD. As part of these actions, the cannabinoid content of some hemp products was determined and many were found to contain levels of CBD largely deviating from the label claim. Like cannabis, hemp oil can be analyzed easily and effectively for its cannabinoid content. This article highlights the use of a fast and simple HPLC–UV assay for separation and quantification of 11 important cannabinoids, including CBD in hemp oil.


Craig Young

Latest:

Fast and Simple High Performance Liquid Chromatography–Ultraviolet Assay for the Determination of Cannabinoid Content in Hemp Oil

The medicinal qualities of cannabinoids contained in hemp have been described in detail. Pain mitigation and reduced severity of nausea and seizures are just a few of the therapeutic benefits reported by medical cannabis patients. There is evidence that a combination of CBD, a host of other minor cannabinoids, and a complex array of terpenoids as contained in hemp oil may be the most beneficial, which is why CBD-rich oil for oral intake has become increasingly popular. The FDA has issued warning letters to firms that market unapproved new drugs allegedly containing CBD. As part of these actions, the cannabinoid content of some hemp products was determined and many were found to contain levels of CBD largely deviating from the label claim. Like cannabis, hemp oil can be analyzed easily and effectively for its cannabinoid content. This article highlights the use of a fast and simple HPLC–UV assay for separation and quantification of 11 important cannabinoids, including CBD in hemp oil.


Flavio A. Franchina

Latest:

Flow-Modulated Comprehensive 2D Gas Chromatography–Triple Quadrupole MS Elucidation of the Fatty Acids and Unsaponifiable Constituents of Oil Derived from Lemon Seeds, A Food-Industry Waste Product

This article is focused on the detailed qualitative analysis of the fatty acids and the unsaponifiable constituents of a vegetable oil derived from a food-industry waste product, namely lemon seeds.


Teresa Pekol

Latest:

Validation of LC–MS-MS Methods for the Determination of Ibuprofen in Miniature Swine Plasma and Synovial Fluid

Published methods for the determination of ibuprofen in biological fluids by liquid chromatography (LC)–UV or LC–mass spectrometry (MS)-MS have quantitation ranges consistent with the relatively high but typical ibuprofen dose (200–800 mg), generally having lower limits of quantitation in the low micrograms-per-milliliter range. For the analysis of plasma and synovial fluid samples from preclinical (miniature swine) studies utilizing a novel ibuprofen dosage form, LC–MS-MS methods were developed and validated over the 10–1000 ng/mL range. Ibuprofen undergoes biotransformation to ibuprofen acyl glucuronide and sublimes under routine bioanalytical sample handling conditions. Procedures were implemented to minimize the impact of these potential liabilities.


Johannes Lenz

Latest:

Analyzing Thermoreversible Bonding and Debonding in Macromolecular Systems by Temperature-Dependent Size-Exclusion Chromatography

The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.


Freidrich Georg Schmidt

Latest:

Analyzing Thermoreversible Bonding and Debonding in Macromolecular Systems by Temperature-Dependent Size-Exclusion Chromatography

The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.


Kai Pahnke

Latest:

Analyzing Thermoreversible Bonding and Debonding in Macromolecular Systems by Temperature-Dependent Size-Exclusion Chromatography

The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.


Christopher Barner-Kowollik

Latest:

Analyzing Thermoreversible Bonding and Debonding in Macromolecular Systems by Temperature-Dependent Size-Exclusion Chromatography

The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.


Josef Brandt

Latest:

Analyzing Thermoreversible Bonding and Debonding in Macromolecular Systems by Temperature-Dependent Size-Exclusion Chromatography

The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.


Mohammed Sharif Khan

Latest:

Pressure Tuning: Increasing the Flexibility of Comprehensive Two-Dimensional Gas Chromatography

Pressure tuning makes it easy to change the orthogonality in the 2D space.


Gesa J. Schad

Latest:

AI-Powered Precision for Functional Component Testing in Tea Analysis

Analyzing functional foods reveals numerous health benefits. These foods are rich in bioactive compounds that go beyond basic nutrition, boosting the immune system and improving overall wellness. However, analyzing these compounds can be challenging. This article discusses AI algorithms to support automated method development for liquid chromatography, simplifying the process, enhancing labor efficiency, and ensuring precise results, making it accessible to non-experts for tea analysis.