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The Use of HILIC Zwitterionic Phase Superficially Porous Particles for Metabolomics AnalysisThis article highlights the use of a new HILIC zwitterionic phase on superficially porous particles. A study on the use of a novel mobile-phase additive to achieve superior peak shape and isomer separation is also discussed, as well as improved LC–MS detection capabilities for metabolomics analysis.
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The Use of HILIC Zwitterionic Phase Superficially Porous Particles for Metabolomics AnalysisThis article highlights the use of a new HILIC zwitterionic phase on superficially porous particles. A study on the use of a novel mobile-phase additive to achieve superior peak shape and isomer separation is also discussed, as well as improved LC–MS detection capabilities for metabolomics analysis.
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Direct Liquid Chromatography Tandem Mass Spectrometry Analysis of Glyphosate, AMPA, Glufosinate, and MPPA in Water Without DerivatizationThis article describes a direct analysis of glyphosate, aminomethylphosphonic acid (AMPA), glufosinate, and 3-methylphosphinicopropionic acid (MPPA) in water by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) without derivatization. The chromatographic separation was performed using a hydrophilic interaction liquid chromatography (HILIC) column and typical LC–MS mobile phases. Method performance was evaluated, showing excellent results. The low limits of quantification (LLOQs) obtained meet the requirements of EU guidelines and could also be used to get an agreement in France where regulations require lower LLOQs (NOR: DEVL1703763V).
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Direct Liquid Chromatography Tandem Mass Spectrometry Analysis of Glyphosate, AMPA, Glufosinate, and MPPA in Water Without DerivatizationThis article describes a direct analysis of glyphosate, aminomethylphosphonic acid (AMPA), glufosinate, and 3-methylphosphinicopropionic acid (MPPA) in water by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) without derivatization. The chromatographic separation was performed using a hydrophilic interaction liquid chromatography (HILIC) column and typical LC–MS mobile phases. Method performance was evaluated, showing excellent results. The low limits of quantification (LLOQs) obtained meet the requirements of EU guidelines and could also be used to get an agreement in France where regulations require lower LLOQs (NOR: DEVL1703763V).
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Two-Dimensional Liquid Chromatography Does More Than Increase Peak CapacityTwo-dimensional liquid chromatography (2D-LC) is drawn out of the chromatographic toolbox if resolution for compounds of interest is insufficient. Recently, several studies have started to highlight 2D-LC as a tool of choice to streamline analytical workflows to increase automation making them less time-consuming. This article highlights two proven cases where 2D-LC does more than simply increase peak capacity.
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Application of Novel Balance Systems: Next Step Towards Laboratory Automatization?The key differences between a classical balance and currently available automated systems from the good weighing practice (GWP) perspective and within the scope of ISO9001:2015 quality standard are discussed. The systems under review are: (i) manual analytical balances; (ii) semi‑automatic systems; (iii) fully automatic systems; and (iv) integrated systems that use on-line coupling of the analytical balance with the instrument used for analytical measurements. The parameters defined in GWP guidelines, such as accuracy, uncertainty, minimum weight, and risks (including out‑of‑specification results), will be evaluated for all of these systems.
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Higher Order Mass Spectrometry Techniques Applied to BiopharmaceuticalsHigher Order Mass Spectrometry Techniques Applied to Biopharmaceuticals
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When Do We Need Sub2m Superficially Porous Particles for Liquid Chromatography SeparationsThe use of superficially porous particles (SPPs) for modern high performance liquid chromatography (HPLC) is now very common. Initially, SPPs rose as an alternative to sub-2-µm fully porous particles (FPPs). In recent years, many column manufacturers have developed 2-µm and smaller SPP-based products. This article investigates the practical utility of these smaller SPP designs.
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The Determination of Trace Per- and Polyfluoroalkyl Substances and Their Precursors Migrated into Food Simulants from Food Contact Materials by Liquid Chromatography–MS/MS and Gas Chromatography–MS/MSAn increasing range of per- and polyfluoroalkyl substances (PFAS) has been found migrating from food contact material into food. This article establishes an integrated analytical approach combining HPLC–MS/MS and GC–MS/MS to detect 36 such PFAS.
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Dispersive Miniaturized Solid‑Phase Extraction Using the CIM‑81 Metal‑Organic Framework and Gas Chromatography–Mass Spectrometry to Determine Personal Care Products in WatersThe analysis of contaminants found in environmental waters and originating from personal care products using metalorganic frameworks (MOFs) combined with liquid chromatography (LC) is described. This work expands the use of MOFs from gas chromatography to LC and also meets the requirements of green analytical chemistry.
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Determination of Polycyclic Aromatic Hydrocarbons in Environmental Waters Using Heterogeneous Magnetic Materials Based on Metal–Organic FrameworksThis study of PAHs makes it easy to compare this microextraction procedure to conventional approaches and to other procedures using magnetic composites.
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Determination of Polycyclic Aromatic Hydrocarbons in Environmental Waters Using Heterogeneous Magnetic Materials Based on Metal–Organic FrameworksThis study of PAHs makes it easy to compare this microextraction procedure to conventional approaches and to other procedures using magnetic composites.
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Dispersive Miniaturized Solid-Phase Extraction Using the CIM-81 Metal-Organic Framework and Gas Chromatography–Mass Spectrometry to Determine Personal Care Products in WatersIn this study, the analysis of contaminants found in environmental waters and originating from personal care products is addressed using metalorganic frameworks (MOFs) in combination with liquid chromatography (LC). This work expands the use of MOFs from gas chromatography to LC and also meets the requirements of green analytical chemistry.
Latest:
Dispersive Miniaturized Solid‑Phase Extraction Using the CIM‑81 Metal‑Organic Framework and Gas Chromatography–Mass Spectrometry to Determine Personal Care Products in WatersThe analysis of contaminants found in environmental waters and originating from personal care products using metalorganic frameworks (MOFs) combined with liquid chromatography (LC) is described. This work expands the use of MOFs from gas chromatography to LC and also meets the requirements of green analytical chemistry.
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Marjorie Groothuis Horning: Biochemist, Pharmacologist, and GC PioneerA look back at this innovator’s career and her efforts to develop and popularize gas chromatography (GC), particularly for biomedical research.
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Mystery “Blobs” Washing Up in Newfoundland May Have Been Identified Through MS“Strange blobs” that started washing up on Newfoundland’s shores months ago may have been identified through a Canadian chemist’s investigation using mass spectrometry (MS).
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DNA Adductomic Analysis by Data-Independent Mass SpectrometryA new type of analysis called “wide-selected ion monitoring (SIM)/MS2” scanning, is capable of screening for a wide range of DNA adducts (chemical modifications to genomic DNA). This method has successfully identified DNA adducts from carcinogen exposures and oxidative stress in human prostate and kidney tissues.
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DNA Adductomic Analysis by Data-Independent Mass SpectrometryA new type of analysis called “wide-selected ion monitoring (SIM)/MS2” scanning, is capable of screening for a wide range of DNA adducts (chemical modifications to genomic DNA). This method has successfully identified DNA adducts from carcinogen exposures and oxidative stress in human prostate and kidney tissues.
Latest:
DNA Adductomic Analysis by Data-Independent Mass SpectrometryA new type of analysis called “wide-selected ion monitoring (SIM)/MS2” scanning, is capable of screening for a wide range of DNA adducts (chemical modifications to genomic DNA). This method has successfully identified DNA adducts from carcinogen exposures and oxidative stress in human prostate and kidney tissues.
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Targeted Metabolomics Analysis of Type 2 Diabetes Serum Samples Using Mass Spectrometry and a Standardized Quantitative MethodAn important advantage of standardized methods is that they enable comparability between laboratories and across studies. In this work, the author used a standardized targeted kit to demonstrate the accuracy, sensitivity, and reproducibility of the approach, analyzing serum samples obtained from type 2 diabetes study subjects and healthy controls.
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Optimizing Splitless GC InjectionsSplitless injections are sometimes necessary for trace analyses, where the analyst hopes to recover 100% of the analytes that are injected. Unfortunately, splitless injections can be challenging and using an imperfect method can lead to loss of analytes and poor peak shapes. The choice of inlet liner can have an impact on the data and one must consider the effects of geometry, packing, deactivation, and volume on introduction of analytes into the system. Other important inlet parameters to consider include inlet temperature, splitless hold time, and initial oven temperature.
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High-Throughput Headspace Analysis of Volatile Impurities in Consumer Products Using Standard AdditionsThe method of standard additions (MoSA) enables quantification of volatile impurities in condensed-phase samples, such as emulsions, for which matrix-matched calibration standards are required. The technique is, however, expensive because it requires multiple analyses of each sample.
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Rapid Classification of Beef Aroma Quality Using SIFT-MSApplication of multivariate statistical analysis to a combined data set demonstrated that SIFT-MS discriminates premium quality beef from eight sensory defects, and, therefore, could be applied as an instrumental grading tool, obviating sensory panel grading.
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High-Throughput Headspace Analysis of Volatile Impurities in Consumer Products Using Standard AdditionsThe method of standard additions (MoSA) enables quantification of volatile impurities in condensed-phase samples, such as emulsions, for which matrix-matched calibration standards are required. The technique is, however, expensive because it requires multiple analyses of each sample.
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Fast and Simple High Performance Liquid Chromatography–Ultraviolet Assay for the Determination of Cannabinoid Content in Hemp OilThe medicinal qualities of cannabinoids contained in hemp have been described in detail. Pain mitigation and reduced severity of nausea and seizures are just a few of the therapeutic benefits reported by medical cannabis patients. There is evidence that a combination of CBD, a host of other minor cannabinoids, and a complex array of terpenoids as contained in hemp oil may be the most beneficial, which is why CBD-rich oil for oral intake has become increasingly popular. The FDA has issued warning letters to firms that market unapproved new drugs allegedly containing CBD. As part of these actions, the cannabinoid content of some hemp products was determined and many were found to contain levels of CBD largely deviating from the label claim. Like cannabis, hemp oil can be analyzed easily and effectively for its cannabinoid content. This article highlights the use of a fast and simple HPLC–UV assay for separation and quantification of 11 important cannabinoids, including CBD in hemp oil.
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Fast and Simple High Performance Liquid Chromatography–Ultraviolet Assay for the Determination of Cannabinoid Content in Hemp OilThe medicinal qualities of cannabinoids contained in hemp have been described in detail. Pain mitigation and reduced severity of nausea and seizures are just a few of the therapeutic benefits reported by medical cannabis patients. There is evidence that a combination of CBD, a host of other minor cannabinoids, and a complex array of terpenoids as contained in hemp oil may be the most beneficial, which is why CBD-rich oil for oral intake has become increasingly popular. The FDA has issued warning letters to firms that market unapproved new drugs allegedly containing CBD. As part of these actions, the cannabinoid content of some hemp products was determined and many were found to contain levels of CBD largely deviating from the label claim. Like cannabis, hemp oil can be analyzed easily and effectively for its cannabinoid content. This article highlights the use of a fast and simple HPLC–UV assay for separation and quantification of 11 important cannabinoids, including CBD in hemp oil.
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Flow-Modulated Comprehensive 2D Gas Chromatography–Triple Quadrupole MS Elucidation of the Fatty Acids and Unsaponifiable Constituents of Oil Derived from Lemon Seeds, A Food-Industry Waste ProductThis article is focused on the detailed qualitative analysis of the fatty acids and the unsaponifiable constituents of a vegetable oil derived from a food-industry waste product, namely lemon seeds.
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Validation of LC–MS-MS Methods for the Determination of Ibuprofen in Miniature Swine Plasma and Synovial FluidPublished methods for the determination of ibuprofen in biological fluids by liquid chromatography (LC)–UV or LC–mass spectrometry (MS)-MS have quantitation ranges consistent with the relatively high but typical ibuprofen dose (200–800 mg), generally having lower limits of quantitation in the low micrograms-per-milliliter range. For the analysis of plasma and synovial fluid samples from preclinical (miniature swine) studies utilizing a novel ibuprofen dosage form, LC–MS-MS methods were developed and validated over the 10–1000 ng/mL range. Ibuprofen undergoes biotransformation to ibuprofen acyl glucuronide and sublimes under routine bioanalytical sample handling conditions. Procedures were implemented to minimize the impact of these potential liabilities.
