The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.
The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.
The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.
The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.
The potential of TD SEC for in situ analyses of thermoreversibly bonded polymers is discussed. TD SEC allows the evolution of the polymer’s molar mass distribution to be monitored during temperature-sensitive bonding and debonding reactions. Through quantitative evaluation of the chromatograms, the reaction-influencing parameters can be studied, which is crucial for the effective development of novel functional materials. By using TD SEC, the effect of polymer size and flexibility on the debonding temperatures of DA polymers was confirmed, their debonding and bonding ability studied, and the de-crosslinking of thermoreversibly cross-linked DA polymers assessed. TD SEC offers a versatile platform for a broad variety of different polymer materials and to assess a variety of different analytical questions.
Pressure tuning makes it easy to change the orthogonality in the 2D space.
Analyzing functional foods reveals numerous health benefits. These foods are rich in bioactive compounds that go beyond basic nutrition, boosting the immune system and improving overall wellness. However, analyzing these compounds can be challenging. This article discusses AI algorithms to support automated method development for liquid chromatography, simplifying the process, enhancing labor efficiency, and ensuring precise results, making it accessible to non-experts for tea analysis.
This episode focuses on alternative modes of liquid chromatography (LC) for oligonucleotides as current go-to chromatographic modes may face significant changes in the future.
A critical look into the future of imaging mass spectrometry is made by addressing some of the fundamental and technical challenges that still need to be overcome.
Native mass spectrometry, the method by which noncovalent protein complexes are retained in the gas phase for intact mass analysis, is gaining interest as a method for intact protein characterization. The development of a modified orbital ion trap platform for high-resolution analyses has expanded the role of native mass spectrometry to address the challenges of intact protein characterization.
Microplastics in water are a major concern, found even in tap water and human bodies. Learn how Sartorius Arium® Pro VF series removes them effectively.
Published methods for the determination of ibuprofen in biological fluids by liquid chromatography (LC)–UV or LC–mass spectrometry (MS)-MS have quantitation ranges consistent with the relatively high but typical ibuprofen dose (200–800 mg), generally having lower limits of quantitation in the low micrograms-per-milliliter range. For the analysis of plasma and synovial fluid samples from preclinical (miniature swine) studies utilizing a novel ibuprofen dosage form, LC–MS-MS methods were developed and validated over the 10–1000 ng/mL range. Ibuprofen undergoes biotransformation to ibuprofen acyl glucuronide and sublimes under routine bioanalytical sample handling conditions. Procedures were implemented to minimize the impact of these potential liabilities.
The parameters that should be considered in the optimization of HPLC methods are explained and then illustrated through the analysis of two commercial agricultural products.
In this review, practical principles and guidelines for designing LCxLC methods are given.
This study presents applications of size-exclusion chromatography (SEC) for characterization and quality control of novel biotherapeutic products, including antibody–drug conjugates, hydrophobic proteins, and coformulations. Examples of modifying SEC mobile-phase composition and running conditions to modulate the separation are discussed, as well as approaches and strategies for analyzing atypical protein products such as coformulations.
This article describes and tests a dynamic DHS−TD−GC−MS method for the fingerprinting analysis of mobile volatile organic compounds in soil.
Analytical separation techniques based on the differential migration velocities of analytes under the action of an electric field are gaining increasing acceptance for the analysis of phenolic compounds in edible and medicinal plants and in plant-derived food products. In Part 2 of this review article the authors discuss the fundamental principles and practical aspects of electromigration techniques, including capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), and capillary electrochromatography (CEC). The development of two-dimensional systems, performed by coupling either liquid chromatography (LC) with an electromigration technique or two electromigration techniques, operated under different separation mechanisms, is also discussed.
Analytical separation techniques based on the differential migration velocities of analytes under the action of an electric field are gaining increasing acceptance for the analysis of phenolic compounds in edible and medicinal plants and in plant-derived food products. In Part 2 of this review article the authors discuss the fundamental principles and practical aspects of electromigration techniques, including capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), and capillary electrochromatography (CEC). The development of two-dimensional systems, performed by coupling either liquid chromatography (LC) with an electromigration technique or two electromigration techniques, operated under different separation mechanisms, is also discussed.
Analytical separation techniques based on the differential migration velocities of analytes under the action of an electric field are gaining increasing acceptance for the analysis of phenolic compounds in edible and medicinal plants and in plant-derived food products. In Part 2 of this review article the authors discuss the fundamental principles and practical aspects of electromigration techniques, including capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), and capillary electrochromatography (CEC). The development of two-dimensional systems, performed by coupling either liquid chromatography (LC) with an electromigration technique or two electromigration techniques, operated under different separation mechanisms, is also discussed.
Analytical separation techniques based on the differential migration velocities of analytes under the action of an electric field are gaining increasing acceptance for the analysis of phenolic compounds in edible and medicinal plants and in plant-derived food products. In Part 2 of this review article the authors discuss the fundamental principles and practical aspects of electromigration techniques, including capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), and capillary electrochromatography (CEC). The development of two-dimensional systems, performed by coupling either liquid chromatography (LC) with an electromigration technique or two electromigration techniques, operated under different separation mechanisms, is also discussed.
The macroscopic properties of material based on poly(D,L-lactic-co-glycolic acid) (PLGA) polymers are tunable by molar mass distribution and degree of branching, enabling optimization for applications in the pharmaceutical and medical industries. Size-exclusion chromatography followed by online multi-angle light scattering with intrinsic viscosity detection (SEC–MALS–IV) is an advanced analytical method for determining absolute molar mass distributions, identifying polymer conformation and quantifying branching. SEC–MALS–IV overcomes the errors that can be encountered in molar mass determined by conventional SEC, which arise from chemical composition and molecular structure, and provides comprehensive characterization of PLGA to facilitate the targeted development of optimized polymer.
This application note outlines the performance benefits achieved with UCT’s LipiFiltr® cleanup cartridge for the analysis of pesticides in oil-based cannabis products using LC–MS/MS analysis.
Phospholipids (PLs) are the major components of cellular membranes. They are important for the functionality of membrane proteins or serve as precursors for second messengers. Several studies reveal the role of PL alterations in various diseases such as cancer (1). Therefore, it is crucial to identify and quantify PLs in complex biological samples for lipidomic studies and clinical research.
This application note shows the pyrolysis-GC×GC–HRMS profiles of crude oils for more detailed separations and more complete characterization of complex matrices, especially on the speciation of heteroatoms such as sulphur-containing compounds.
This proof-of-principle study shows that polymer-based sorptive extraction probes, coupled with secondary focusing by thermal desorption and analysis by flow-modulated GC×GC–TOF-MS/FID, can be used to separate and identify flavour compounds in milk. As well as comparing the profiles of dairy and non-dairy milks, this article highlights the practical benefits of this sampling procedure, the ability of two-dimensional GC to physically separate components that would coelute in one-dimensional GC, and the use of software tools to improve workflow.
Approximately 40% of recombinant proteins that are purified use a histidine tag for easy capture. This article covers how to automate the purification of histidine-tagged proteins and how purification conditions can be optimized to an automated four-step purification scheme that uses affinity-, ion exchange-, and size-exclusion columns. Using a multistep purification scheme removes the manual steps that cause loss of precious proteins and take more time, like dialysis, collection, and reinjecting samples. The final purification scheme reduces a 3–4-day process to 11.5 h from start to finish, all while improving reproducibility, yield, and comparable purity.
Approximately 40% of recombinant proteins that are purified use a histidine tag for easy capture. This article covers how to automate the purification of histidine-tagged proteins and how purification conditions can be optimized to an automated four-step purification scheme that uses affinity-, ion exchange-, and size-exclusion columns. Using a multistep purification scheme removes the manual steps that cause loss of precious proteins and take more time, like dialysis, collection, and reinjecting samples. The final purification scheme reduces a 3–4-day process to 11.5 h from start to finish, all while improving reproducibility, yield, and comparable purity.
Gel Permeation Chromatography (GPC) is widely used for sample clean up in mycotoxin analysis. The most commonly described methods use GPC columns packed with SX-3 BioBeads suitable for cleaning Zearalenone, Aflatoxins, and Trichothesenes from edible oils and fatty matrices. Separation of Fumonisins from the oil fraction are inadequate with this column.
A fast, selective, and reproducible high performance liquid chromatography (HPLC) method was developed and validated for the analyses of third-generation cephalosporin antibiotics, namely, ceftriaxone, cefixime, and cefdinir in human plasma. The analysis was carried out on a 150 mm Ã- 4.6 mm, 5.0-µm C18 column. The mobile phase used was 80:20 (v/v) 50 mmM phosphate buffer (pH 5.0)–methanol at a flow rate of 1.0 mL/min with 230-nm UV detection.