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Macherey-Nagel - Determination of Triphenylmethane Dyes from Aquaculture SamplesThis application note describes the determination of the triphenylmethane dyes Malachite Green and Crystal Violet and their metabolites from the aquaculture samples brown trout, shrimp, and tuna using dispersive SPE (dSPE) with CHROMABOND QuEChERS mixes for sample clean-up.
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Determination of Pharmaceuticals from SerumThis application note describes the determination of pharmaceuticals from serum using solid-phase extraction (SPE) with the hydrophilic-lipophilic balanced SPE phase CHROMABOND® HLB for analyte enrichment and for sample cleanup. The eluates from SPE are finally analyzed by HPLC–MS/MS on a NUCLEOSHELL® PFP core–shell phase.
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Macherey-Nagel - Determination of Triphenylmethane Dyes from Aquaculture SamplesThis application note describes the determination of the triphenylmethane dyes Malachite Green and Crystal Violet and their metabolites from the aquaculture samples brown trout, shrimp, and tuna using dispersive SPE (dSPE) with CHROMABOND QuEChERS mixes for sample clean-up.
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Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS in Potable and Non-Potable WatersA hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.
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Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS in Potable and Non-Potable WatersA hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.
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The Benefits of AQbDThe benefits of an analytical quality by design (AQbD) approach to method development cannot be underestimated. The Column spoke to Changqin Hu and Xia Zhang from the National Institutes for Food Drug Control, in Beijing, China, about their work developing a dual-gradient elution stability-indicating method for cloxacillin within an AQbD framework.
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The Benefits of AQbDThe benefits of an analytical quality by design (AQbD) approach to method development cannot be underestimated. The Column spoke to Changqin Hu and Xia Zhang from the National Institutes for Food Drug Control, in Beijing, China, about their work developing a dual-gradient elution stability-indicating method for cloxacillin within an AQbD framework.
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The Power of Trypsin Immobilized Enzyme Reactors (IMERs) Deployed in Online MDLC–MS ApplicationsCommercially available trypsin IMERs can digest proteins with high sequence coverage and robustness, facilitating online multidimensional LC–MS.
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Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Performance Liquid Chromatography–Mass SpectrometryA fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent QTOF-MS characterization is presented. The technique is based on state-of-the-art 2D-HPLC technology coupled with additional HPLC modules via a dedicated software macro.
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Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Performance Liquid Chromatography–Mass SpectrometryA fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent QTOF-MS characterization is presented. The technique is based on state-of-the-art 2D-HPLC technology coupled with additional HPLC modules via a dedicated software macro.
Latest:
Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Performance Liquid Chromatography–Mass SpectrometryA fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent QTOF-MS characterization is presented. The technique is based on state-of-the-art 2D-HPLC technology coupled with additional HPLC modules via a dedicated software macro.
Latest:
Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Performance Liquid Chromatography–Mass SpectrometryA fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent QTOF-MS characterization is presented. The technique is based on state-of-the-art 2D-HPLC technology coupled with additional HPLC modules via a dedicated software macro.
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Eluent Preparation for Hydrophilic Interaction Liquid Chromatography, Part 1: Solvent Volumes and Buffer CounterionsPaying attention to the details of mobile-phase preparation can have a big impact on the reproducibility of hydrophilic-interaction chromatography (HILIC) separations.
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Column Re-Equilibration Following Gradient Elution: How Long is Long Enough? Part 1: Reversed-Phase and HILIC Separations of Small MoleculesFor decades the prevailing perception was that satisfactory re-equilibration of reversed-phase columns following gradient elution took a long time. In the early 2000s we showed that this perception was not well founded, and demonstrated that adequate re‑equilibration could be achieved in seconds. Recently, we have shown the same for HILIC columns. All of this work so far has been with small molecules. In this article, we present an overview of this work, and summarize the practical utility of it all.
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Comparing Multivariate Eluent Design Spaces for Systematic Characterization of (U)HPLC ColumnsPresenting a novel chromatographic modelling method to establish QbD-compliant comparative testing of eluent design spaces.
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Development and Validation of an HPTLC Method for Determination of Aflatoxin B1A high performance thin-layer chromatographic (HPTLC) method was developed for the determination of aflatoxin B1 in cereals.
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Peak Purity in Liquid Chromatography, Part 2: Potential of Curve Resolution TechniquesIs that peak “pure”? How do I know if there might be something hiding under there?
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What Can Two-Dimensional Liquid Chromatography Offer the Pharmaceutical Industry?The evolution of two-dimensional liquid chromatography (2D-LC) instruments along with improved software capabilities has transferred 2D-LC from the hands of experienced researchers to functioning analytical laboratories in the pharmaceutical industry. 2D-LC offers chromatographers novel solutions to problems ranging from analyzing complex samples requiring excessively large peak capacities to separating simple compounds that are difficult to resolve. Recent developments in 2D-LC and 2D-LC–MS have demonstrated the potential of this technique in practice and 2D-LC is set to become an essential tool in the pharmaceutical sector to address problems ranging from coelution, peak purity assessment, simultaneous achiral-chiral analysis, genotoxic impurities, and more.
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Implementation of Methods Translation between Liquid Chromatography InstrumentationPharmaceutical research and development (R&D) organizations were early adopters who recognized the many benefits of UltraPerformance LC? (UPLC?) Technology including resolution, sensitivity, throughput, and productivity as compared to HPLC.
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Stationary-Phase Optimized Selectivity in Liquid Chromatography (SOS-LC) for Pharmaceutical AnalysisChromatographic method development for pharmaceutical analysis can benefit from in silico steered serial coupling of column segments containing different stationary phases of varying length. Contrary to column coupling through trial and error, in stationary-phase optimized selectivity (SOS)-based chromatography the retention of all solutes is predicted for all possible column combinations allowing a rational selection of the optimal column combination. The possibilities of the strategy now surpass the initial usage in isocratic high performance liquid chromatography (HPLC) on dedicated commercial column segments, and allow applications in gradient-, green-, preparative-, and in supercritical fluid chromatography (SFC) on conventional column hardware. Current possibilities, pharmaceutical applications, a downloadable algorithm, and weaknesses of the approach are discussed to allow broader implementation of this methodology in separation science.
Latest:
Stationary-Phase Optimized Selectivity in Liquid Chromatography (SOS-LC) for Pharmaceutical AnalysisChromatographic method development for pharmaceutical analysis can benefit from in silico steered serial coupling of column segments containing different stationary phases of varying length. Contrary to column coupling through trial and error, in stationary-phase optimized selectivity (SOS)-based chromatography the retention of all solutes is predicted for all possible column combinations allowing a rational selection of the optimal column combination. The possibilities of the strategy now surpass the initial usage in isocratic high performance liquid chromatography (HPLC) on dedicated commercial column segments, and allow applications in gradient-, green-, preparative-, and in supercritical fluid chromatography (SFC) on conventional column hardware. Current possibilities, pharmaceutical applications, a downloadable algorithm, and weaknesses of the approach are discussed to allow broader implementation of this methodology in separation science.
Latest:
Stationary-Phase Optimized Selectivity in Liquid Chromatography (SOS-LC) for Pharmaceutical AnalysisChromatographic method development for pharmaceutical analysis can benefit from in silico steered serial coupling of column segments containing different stationary phases of varying length. Contrary to column coupling through trial and error, in stationary-phase optimized selectivity (SOS)-based chromatography the retention of all solutes is predicted for all possible column combinations allowing a rational selection of the optimal column combination. The possibilities of the strategy now surpass the initial usage in isocratic high performance liquid chromatography (HPLC) on dedicated commercial column segments, and allow applications in gradient-, green-, preparative-, and in supercritical fluid chromatography (SFC) on conventional column hardware. Current possibilities, pharmaceutical applications, a downloadable algorithm, and weaknesses of the approach are discussed to allow broader implementation of this methodology in separation science.
Latest:
Stationary-Phase Optimized Selectivity in Liquid Chromatography (SOS-LC) for Pharmaceutical AnalysisChromatographic method development for pharmaceutical analysis can benefit from in silico steered serial coupling of column segments containing different stationary phases of varying length. Contrary to column coupling through trial and error, in stationary-phase optimized selectivity (SOS)-based chromatography the retention of all solutes is predicted for all possible column combinations allowing a rational selection of the optimal column combination. The possibilities of the strategy now surpass the initial usage in isocratic high performance liquid chromatography (HPLC) on dedicated commercial column segments, and allow applications in gradient-, green-, preparative-, and in supercritical fluid chromatography (SFC) on conventional column hardware. Current possibilities, pharmaceutical applications, a downloadable algorithm, and weaknesses of the approach are discussed to allow broader implementation of this methodology in separation science.
Latest:
Copenhagen Symposium on Separation Sciences (CSSS) Event PreviewThe 2nd Copenhagen Symposium on Separation Sciences (CSSS 2018) will be held on 26–27 June 2018 at the DGI-byen in Copenhagen, Denmark. The preliminary programme of the 2nd CSSS is summarized below and gives an idea of what can be expected.
Latest:
Copenhagen Symposium on Separation Sciences (CSSS) Event PreviewThe 2nd Copenhagen Symposium on Separation Sciences (CSSS 2018) will be held on 26–27 June 2018 at the DGI-byen in Copenhagen, Denmark. The preliminary programme of the 2nd CSSS is summarized below and gives an idea of what can be expected.
Latest:
Copenhagen Symposium on Separation Sciences (CSSS) Event PreviewThe 2nd Copenhagen Symposium on Separation Sciences (CSSS 2018) will be held on 26–27 June 2018 at the DGI-byen in Copenhagen, Denmark. The preliminary programme of the 2nd CSSS is summarized below and gives an idea of what can be expected.
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Quantitative Multi-Target Screening Using Library-Based IdentificationThis article presents a novel approach for toxicology and pharmacology laboratories that combines multi-compound screening followed by multi-compound determination in a single method. The multi-compound assay developed allows 90 molecules including benzodiazepines, cocaine and related stimulants, amphetamines, and opioids to be measured. The multi-targeted screening (MTS) method was developed using a spectra library containing over 1200 compounds. The approach was evaluated in a routine clinical toxicology laboratory to detect and quantify compounds in unknown samples.
Latest:
Copenhagen Symposium on Separation Sciences (CSSS) Event PreviewThe 2nd Copenhagen Symposium on Separation Sciences (CSSS 2018) will be held on 26–27 June 2018 at the DGI-byen in Copenhagen, Denmark. The preliminary programme of the 2nd CSSS is summarized below and gives an idea of what can be expected.
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Anomalous Retention Prediction Using Modelling Software in Gradient Reversed-Phase Liquid Chromatography: Why it Can Occur and How to Prevent ItThe success of screening column and mobile phase combinations that generate dissimilar selectivity is highlighted in a typical method development strategy.
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A Practical Approach to Modelling of Reversed-Phase Liquid Chromatographic Separations: Advantages, Principles, and Possible PitfallsChromatographic principles and best practices for obtaining highly precise retention time, peak width, and resolution predictions for the optimization of reversed-phase liquid chromatography (LC) separations using retention modelling software will be discussed. The importance of fully characterizing the LC instrumentation, how to generate accurate input data, the selection of appropriate models, and peak tracking will be addressed along with a suggested workflow. Adhesion to a few basic rules and simple precautions and the use of modern retention modelling software programmes can assist the rapid development of highly accurate retention models to enable the development of robust and optimized reversed-phase LC separations using either ultrahigh-pressure liquid chromatography (UHPLC) or high performance liquid chromatography (HPLC) conditions. Examples of retention modelling for small and large molecules will be highlighted.
