Author


Minna Kallio

Latest:

Multidimensional and Hyphenated Techniques in Aerosol Analysis

The authors provide suggestions for the sample pretreatment and analysis of aerosol particles including the use of on-line SFE–LC–GC–MS, GCXGC and LC–MS. Although each technique has its merits, the novel multidimensional systems and those integrating sample pretreatment with the final analysis proved to be valuable tools in aerosol analysis.


Masahiko Shimmo

Latest:

Multidimensional and Hyphenated Techniques in Aerosol Analysis

The authors provide suggestions for the sample pretreatment and analysis of aerosol particles including the use of on-line SFE–LC–GC–MS, GCXGC and LC–MS. Although each technique has its merits, the novel multidimensional systems and those integrating sample pretreatment with the final analysis proved to be valuable tools in aerosol analysis.


Alex Hodgson

Latest:

Vacuum Ultraviolet Spectroscopy: A New Tool for Gas Chromatography Analysis of Terpenes in Flavours and Fragrances

Terpenes contribute heavily to the senses of smell and taste and thus are integral to industries like herb and spice producers, essential oil manufacturers, cannabis growers and distributors, breweries, and distilleries, among countless others. Current terpene analysis is performed using gas chromatography–flame ionization detection (GC–FID) and GC–mass spectrometry (MS); however, baseline separation is needed for quantification because many terpenes of interest are isomers, which can lead to relatively long run times. Vacuum ultraviolet (VUV) spectroscopy can spectrally distinguish isomers and quantitatively deconvolve coeluting peaks, allowing for significant reduction in GC run time. This article outlines a method for the analysis of 21 terpenes in a variety of samples with a sub 9-min elution time.


Martin Winter

Latest:

The Power of Trypsin Immobilized Enzyme Reactors (IMERs) Deployed in Online MDLC–MS Applications

Commercially available trypsin IMERs can digest proteins with high sequence coverage and robustness, facilitating online multidimensional LC–MS.


Sascha Nowak

Latest:

Analysis of Organophosphates in Lithium Ion Battery Electrolytes by HILIC–ESI-MS

New separation techniques for the analysis of polar and ionic analytes have aroused great interest in the field of metabolomics and environmental investigation in the past two decades. Hydrophilic interaction liquid chromatography (HILIC) is a promising tool to address this challenge. HILIC separation is based on the polarity of analytes, which generally show stronger retention with increasing polarity according to the HILIC separation mechanism. Furthermore, the high content of organic solvent in the mobile phase leads to good ionization properties in the electrospray ionization (ESI), and consequently enhances the detection sensitivity by hyphenated mass spectrometry (MS) detector.


Matteo Volpi

Latest:

Overcome the Memory Effect in Dioxins Extraction with ETHOS X Powered by FastEX-24

Microwave extraction becomes faster and easier with the new Milestone ETHOS X with its FastEX-24. This application note shows the use of Milestone technology for extraction of dioxins from environmental matrices, with emphasis on one of the most common challenge of this application: the carryover effect. The unique design of the FastEX-24 rotor with disposable glass vials allows easy and efficient dioxin extraction, and other organic pollutants, to be performed, avoiding any memory effect. The FastEX-24 simplifies the routine pollutants extraction process and provides superior productivity at lower costs.


Diego Carnaroglio

Latest:

Overcome the Memory Effect in Dioxins Extraction with ETHOS X Powered by FastEX-24

Microwave extraction becomes faster and easier with the new Milestone ETHOS X with its FastEX-24. This application note shows the use of Milestone technology for extraction of dioxins from environmental matrices, with emphasis on one of the most common challenge of this application: the carryover effect. The unique design of the FastEX-24 rotor with disposable glass vials allows easy and efficient dioxin extraction, and other organic pollutants, to be performed, avoiding any memory effect. The FastEX-24 simplifies the routine pollutants extraction process and provides superior productivity at lower costs.


Johannes Brand

Latest:

Macherey-Nagel - Determination of Triphenylmethane Dyes from Aquaculture Samples

This application note describes the determination of the triphenylmethane dyes Malachite Green and Crystal Violet and their metabolites from the aquaculture samples brown trout, shrimp, and tuna using dispersive SPE (dSPE) with CHROMABOND QuEChERS mixes for sample clean-up.


Detlef Lambrecht

Latest:

Determination of Pharmaceuticals from Serum

This application note describes the determination of pharmaceuticals from serum using solid-phase extraction (SPE) with the hydrophilic-lipophilic balanced SPE phase CHROMABOND® HLB for analyte enrichment and for sample cleanup. The eluates from SPE are finally analyzed by HPLC–MS/MS on a NUCLEOSHELL® PFP core–shell phase.


Hans Rainer Wollseifen

Latest:

Macherey-Nagel - Determination of Triphenylmethane Dyes from Aquaculture Samples

This application note describes the determination of the triphenylmethane dyes Malachite Green and Crystal Violet and their metabolites from the aquaculture samples brown trout, shrimp, and tuna using dispersive SPE (dSPE) with CHROMABOND QuEChERS mixes for sample clean-up.


Frances Carroll

Latest:

Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS in Potable and Non-Potable Waters

A hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.


Justin Steimling

Latest:

Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS in Potable and Non-Potable Waters

A hybrid HILIC–ion exchange column was used for the analysis of ultrashort-chain per- and polyfluoroalkyl substances (PFAS) compounds in environmental waters. This direct injection LC–MS method enables simultaneous measurement of ultrashort- chain, alternative, and legacy PFAS in potable and non-potable waters.


Xia Zhang

Latest:

The Benefits of AQbD

The benefits of an analytical quality by design (AQbD) approach to method development cannot be underestimated. The Column spoke to Changqin Hu and Xia Zhang from the National Institutes for Food Drug Control, in Beijing, China, about their work developing a dual-gradient elution stability-indicating method for cloxacillin within an AQbD framework.


Changqin Hu

Latest:

The Benefits of AQbD

The benefits of an analytical quality by design (AQbD) approach to method development cannot be underestimated. The Column spoke to Changqin Hu and Xia Zhang from the National Institutes for Food Drug Control, in Beijing, China, about their work developing a dual-gradient elution stability-indicating method for cloxacillin within an AQbD framework.


Anja Bathke

Latest:

The Power of Trypsin Immobilized Enzyme Reactors (IMERs) Deployed in Online MDLC–MS Applications

Commercially available trypsin IMERs can digest proteins with high sequence coverage and robustness, facilitating online multidimensional LC–MS.


Christian Bell

Latest:

Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Performance Liquid Chromatography–Mass Spectrometry

A fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent QTOF-MS characterization is presented. The technique is based on state-of-the-art 2D-HPLC technology coupled with additional HPLC modules via a dedicated software macro.


Christoph Gstöttner

Latest:

Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Performance Liquid Chromatography–Mass Spectrometry

A fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent QTOF-MS characterization is presented. The technique is based on state-of-the-art 2D-HPLC technology coupled with additional HPLC modules via a dedicated software macro.


Robert Kopf

Latest:

Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Performance Liquid Chromatography–Mass Spectrometry

A fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent QTOF-MS characterization is presented. The technique is based on state-of-the-art 2D-HPLC technology coupled with additional HPLC modules via a dedicated software macro.


Denis Klemm

Latest:

Rapid Online Reduction and Characterization of Protein Modifications Using Fully Automated Two-Dimensional High Performance Liquid Chromatography–Mass Spectrometry

A fully automated process for online peak fractionation and reduction of therapeutic antibodies with subsequent QTOF-MS characterization is presented. The technique is based on state-of-the-art 2D-HPLC technology coupled with additional HPLC modules via a dedicated software macro.


Caley B. Craven

Latest:

Eluent Preparation for Hydrophilic Interaction Liquid Chromatography, Part 1: Solvent Volumes and Buffer Counterions

Paying attention to the details of mobile-phase preparation can have a big impact on the reproducibility of hydrophilic-interaction chromatography (HILIC) separations.


Claudia Seidl

Latest:

Column Re-Equilibration Following Gradient Elution: How Long is Long Enough? Part 1: Reversed-Phase and HILIC Separations of Small Molecules

For decades the prevailing perception was that satisfactory re-equilibration of reversed-phase columns following gradient elution took a long time. In the early 2000s we showed that this perception was not well founded, and demonstrated that adequate re‑equilibration could be achieved in seconds. Recently, we have shown the same for HILIC columns. All of this work so far has been with small molecules. In this article, we present an overview of this work, and summarize the practical utility of it all.


Arnold Zoldhegyi

Latest:

Comparing Multivariate Eluent Design Spaces for Systematic Characterization of (U)HPLC Columns

Presenting a novel chromatographic modelling method to establish QbD-compliant comparative testing of eluent design spaces.


Li Chen

Latest:

Development and Validation of an HPTLC Method for Determination of Aflatoxin B1

A high performance thin-layer chromatographic (HPTLC) method was developed for the determination of aflatoxin B1 in cereals.


Sarah C. Rutan

Latest:

Peak Purity in Liquid Chromatography, Part 2: Potential of Curve Resolution Techniques

Is that peak “pure”? How do I know if there might be something hiding under there?


C.J. Venkatramani

Latest:

What Can Two-Dimensional Liquid Chromatography Offer the Pharmaceutical Industry?

The evolution of two-dimensional liquid chromatography (2D-LC) instruments along with improved software capabilities has transferred 2D-LC from the hands of experienced researchers to functioning analytical laboratories in the pharmaceutical industry. 2D-LC offers chromatographers novel solutions to problems ranging from analyzing complex samples requiring excessively large peak capacities to separating simple compounds that are difficult to resolve. Recent developments in 2D-LC and 2D-LC–MS have demonstrated the potential of this technique in practice and 2D-LC is set to become an essential tool in the pharmaceutical sector to address problems ranging from coelution, peak purity assessment, simultaneous achiral-chiral analysis, genotoxic impurities, and more.


Peter Alden

Latest:

Implementation of Methods Translation between Liquid Chromatography Instrumentation

Pharmaceutical research and development (R&D) organizations were early adopters who recognized the many benefits of UltraPerformance LC? (UPLC?) Technology including resolution, sensitivity, throughput, and productivity as compared to HPLC.


Maarten De Beer

Latest:

Stationary-Phase Optimized Selectivity in Liquid Chromatography (SOS-LC) for Pharmaceutical Analysis

Chromatographic method development for pharmaceutical analysis can benefit from in silico steered serial coupling of column segments containing different stationary phases of varying length. Contrary to column coupling through trial and error, in stationary-phase optimized selectivity (SOS)-based chromatography the retention of all solutes is predicted for all possible column combinations allowing a rational selection of the optimal column combination. The possibilities of the strategy now surpass the initial usage in isocratic high performance liquid chromatography (HPLC) on dedicated commercial column segments, and allow applications in gradient-, green-, preparative-, and in supercritical fluid chromatography (SFC) on conventional column hardware. Current possibilities, pharmaceutical applications, a downloadable algorithm, and weaknesses of the approach are discussed to allow broader implementation of this methodology in separation science.


Ravindra Hegade

Latest:

Stationary-Phase Optimized Selectivity in Liquid Chromatography (SOS-LC) for Pharmaceutical Analysis

Chromatographic method development for pharmaceutical analysis can benefit from in silico steered serial coupling of column segments containing different stationary phases of varying length. Contrary to column coupling through trial and error, in stationary-phase optimized selectivity (SOS)-based chromatography the retention of all solutes is predicted for all possible column combinations allowing a rational selection of the optimal column combination. The possibilities of the strategy now surpass the initial usage in isocratic high performance liquid chromatography (HPLC) on dedicated commercial column segments, and allow applications in gradient-, green-, preparative-, and in supercritical fluid chromatography (SFC) on conventional column hardware. Current possibilities, pharmaceutical applications, a downloadable algorithm, and weaknesses of the approach are discussed to allow broader implementation of this methodology in separation science.


Sander Delahaye

Latest:

Stationary-Phase Optimized Selectivity in Liquid Chromatography (SOS-LC) for Pharmaceutical Analysis

Chromatographic method development for pharmaceutical analysis can benefit from in silico steered serial coupling of column segments containing different stationary phases of varying length. Contrary to column coupling through trial and error, in stationary-phase optimized selectivity (SOS)-based chromatography the retention of all solutes is predicted for all possible column combinations allowing a rational selection of the optimal column combination. The possibilities of the strategy now surpass the initial usage in isocratic high performance liquid chromatography (HPLC) on dedicated commercial column segments, and allow applications in gradient-, green-, preparative-, and in supercritical fluid chromatography (SFC) on conventional column hardware. Current possibilities, pharmaceutical applications, a downloadable algorithm, and weaknesses of the approach are discussed to allow broader implementation of this methodology in separation science.


Kai Chen

Latest:

Stationary-Phase Optimized Selectivity in Liquid Chromatography (SOS-LC) for Pharmaceutical Analysis

Chromatographic method development for pharmaceutical analysis can benefit from in silico steered serial coupling of column segments containing different stationary phases of varying length. Contrary to column coupling through trial and error, in stationary-phase optimized selectivity (SOS)-based chromatography the retention of all solutes is predicted for all possible column combinations allowing a rational selection of the optimal column combination. The possibilities of the strategy now surpass the initial usage in isocratic high performance liquid chromatography (HPLC) on dedicated commercial column segments, and allow applications in gradient-, green-, preparative-, and in supercritical fluid chromatography (SFC) on conventional column hardware. Current possibilities, pharmaceutical applications, a downloadable algorithm, and weaknesses of the approach are discussed to allow broader implementation of this methodology in separation science.