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AI and GenAI Applications to Help Optimize Purification and Yield of Antibodies From Plasma (October 2024)Deriving antibodies from plasma involves a multi-step process, from plasma collection to antibody purification and quality control, ensuring the final product is safe and effective for therapeutic, diagnostic, or research use. Krish Gosh, PhD, MBA, President of TCG Digital, joined LCGC International to discuss how AI/GenAI applications can optimize several steps to enhance yield, profitability, and process monitoring.
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LC/LC–MSA snapshot of key trends and developments in the chromatography sector according to selected panelists from companies exhibiting at Analytica 2018.
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LC/LC–MSA snapshot of key trends and developments in the chromatography sector according to selected panelists from companies exhibiting at Analytica 2018.
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GC/GC–MSA snapshot of key trends and developments in the GC/GC–MS sector according to selected panelists from companies exhibiting at Analytica 2018.
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Data HandlingA snapshot of key trends and developments in the data handling sector according to selected panelists from companies exhibiting at Analytica 2018.
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Can We Continue to Draw the Line?Chromatographic peak integration continues to be a major regulatory issue and was first discussed in this column in 2015. Is the approach to manual intervention and manual integration outlined still acceptable in the light of regulatory citations and guidance documents published since then?
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Developments in Green ChromatographyWhy should you be concerned about mobile-phase degassing - it's all done automatically, isn't it?
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Determination of Seven Sodium Alkyl Sulphates in Environmental Water by Ion-pair Chromatography with Suppressed Conductivity DetectionA simple and fast ion-pair chromatography method to detect sodium alkyl sulphates in environmental samples using conductivity detection is described.
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Automating Analysis of Pain Management Drugs in UrineThis article discusses the benef ts of using automated dispersive solid-phase extraction (dSPE) rather than standard SPE in urine analysis.
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Host Cell Protein Monitoring During Downstream Processing Using Micro-Pillar Array Columns Combined with Mass SpectrometryThis article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry.
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Liquid Chromatography Tandem Mass Spectrometry Method for Novel Psychoactive Substances: Kratom and Synthetic Cathinones in UrineA novel “dilute-and-shoot” LC–MS/MS method is described for the analysis of “bath salts” sold as “legal” highs, including mitragynine and nine synthetic cathinones, in urine.
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Liquid Chromatography Tandem Mass Spectrometry Method for Novel Psychoactive Substances: Kratom and Synthetic Cathinones in UrineA novel “dilute-and-shoot” LC–MS/MS method is described for the analysis of “bath salts” sold as “legal” highs, including mitragynine and nine synthetic cathinones, in urine.
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Liquid Chromatography Tandem Mass Spectrometry Method for Novel Psychoactive Substances: Kratom and Synthetic Cathinones in UrineA novel “dilute-and-shoot” LC–MS/MS method is described for the analysis of “bath salts” sold as “legal” highs, including mitragynine and nine synthetic cathinones, in urine.
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Combining Novel and Traditional Ionization Methods for Mass Spectrometry for More Comprehensive AnalysesNovel ionization processes provide gas-phase ions of a wide variety of materials using MS. These simple and sensitive methods operate from solution or a solid matrix. Both manual and automated platforms are described that allow rapid switching between the ionization methods of MAI, SAI, vSAI, and conventional ESI.
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Combining Novel and Traditional Ionization Methods for Mass Spectrometry for More Comprehensive AnalysesNovel ionization processes provide gas-phase ions of a wide variety of materials using MS. These simple and sensitive methods operate from solution or a solid matrix. Both manual and automated platforms are described that allow rapid switching between the ionization methods of MAI, SAI, vSAI, and conventional ESI.
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Combining Novel and Traditional Ionization Methods for Mass Spectrometry for More Comprehensive AnalysesNovel ionization processes provide gas-phase ions of a wide variety of materials using MS. These simple and sensitive methods operate from solution or a solid matrix. Both manual and automated platforms are described that allow rapid switching between the ionization methods of MAI, SAI, vSAI, and conventional ESI.
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Combining Novel and Traditional Ionization Methods for Mass Spectrometry for More Comprehensive AnalysesNovel ionization processes provide gas-phase ions of a wide variety of materials using MS. These simple and sensitive methods operate from solution or a solid matrix. Both manual and automated platforms are described that allow rapid switching between the ionization methods of MAI, SAI, vSAI, and conventional ESI.
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Combining Novel and Traditional Ionization Methods for Mass Spectrometry for More Comprehensive AnalysesNovel ionization processes provide gas-phase ions of a wide variety of materials using MS. These simple and sensitive methods operate from solution or a solid matrix. Both manual and automated platforms are described that allow rapid switching between the ionization methods of MAI, SAI, vSAI, and conventional ESI.
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Haloacetic Acid Analysis Using Two-Dimensional Matrix-Elimination Ion ChromatographyThe disinfectants commonly used to treat public drinking water can react with naturally occurring organic and inorganic matter in the source water to form disinfection byproducts such as haloacetic acids. Here, we describe the use of two-dimensional matrix-elimination ion chromatography (MEIC) for haloacetic acid analysis. This method minimizes the impact of matrix ions.
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Scaling LC Methods Using Porous Particle Stationary PhasesThe use of ultrahigh-pressure liquid chromatography (UHPLC) is now commonplace among pharmaceutical laboratories. However, until depreciation cycles replace traditional high performance liquid chromatography (HPLC) systems that operate at a maximum pressure of 400 bar, the advantages of UHPLC cannot be realized worldwide. Thus, product methods developed using UHPLC capabilities cannot directly transfer these methods to receiving laboratories without qualified UHPLC availability.
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Hot Topics in HPLC Part I:This is the first in a series of articles exploring topics that will be addressed at the HPLC 2020 conference in San Diego, from June 20 to 25.
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Mass Spectrometry of Organic Molecules and Laser-Induced Acoustic Desorption: Applications, Mechanisms, and PerspectivesThis study illustrates the versatility of a laser-induced acoustic desorption (LIAD) approach for volatilization of analytes in modern mass spectrometry.
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Light Scattering for the Masses Monoclonal Antibodies and the Preparative Use of Flow Field-Flow Fractionation (AF4)Proteins - especially monoclonal antibodies (MABs) - have become increasingly important in pharmaceutical work. However, there are some important differences between conventional, chemically-synthesized drugs and proteins. Because of the complex and weak structure of proteins, even a slight change in conditions, such as pH value, temperature, or mechanical stress, may lead to aggregation and a loss of activity or stability.
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Ion Mobility–Mass Spectrometry for Food Analysis: An UpdateIn food analysis, many different biological matrices are investigated containing numerous compounds that can interfere with liquid chromatographyÐmass spectrometry (LC–MS) analysis. To overcome the challenges that arise with these highly complex matrices, the additional separation of analytes and matrix compounds complementing chromatographic separation is becoming more significant. In this article, the potential of IM-MS to increase selectivity and for additional identity confirmation is investigated. An extensive evaluation of IM-MS instruments was performed on a broad test set of food safety contaminants. The tested IM-MS platforms were DMS, TWIMS, low field DTIMS, and TIMS. CCS data were determined using the different instruments, and the ability to separate isomers and compounds of interest from sample matrix in the IM dimension was explored.
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Automating Analysis of Pain Management Drugs in UrineThis article discusses the benef ts of using automated dispersive solid-phase extraction (dSPE) rather than standard SPE in urine analysis.
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Peak Purity in Liquid Chromatography, Part 2: Potential of Curve Resolution TechniquesIs that peak “pure”? How do I know if there might be something hiding under there?
