Author
Latest:
Liquid Chromatography Methods for the Separation of Short RNA OligonucleotidesSynthetic oligonucleotides have become increasingly popular as a result of the recent discovery of ribonucleic acid interference (RNAi), a natural process for silencing gene expression.
Latest:
Supercritical Fluid Chromatography: A Workhorse in Drug DiscoveryIn this LCGC Blog, Daipayan Roy and Muhammed Qamar Farooq of Amgen discuss the history and impact of supercritical fluid chromatography (SFC) throughout the years.
Latest:
Frontiers in Ultrafast Chiral ChromatographyWe explore the developments that have made it possible to produce sub-minute, and, more recently, even sub-second enantiomeric separations. We also look at the questions that remain unanswered.
Latest:
In Search of The Needle in The Mariana Trench: Host Cell Proteins and the Problem of Dynamic RangeThis article explores the analytical challenges associated with HCP monitoring and reviews recent advances in HCP characterization, with special emphasis on high performance liquid chromatography mass spectrometry (HPLC–MS)-based methods and HCP enrichment techniques.
Latest:
Monitoring of Oxidation in Biopharmaceuticals with Top-to-Bottom High Performance Liquid Chromatography–Mass Spectrometry Methodologies: A Critical CheckFederal regulations concerning the safety and efficacy of biopharmaceuticals require the implementation of a comprehensive toolbox of physicochemical and biological characterization methods. In order to demonstrate consistent overall structure, even minute differences in primary structure and post‑translational modifications (PTMs) have to be detectable in therapeutic proteins. Because of their remarkable capability of revealing small changes in molecular structure, high performance liquid chromatography (HPLC) and mass spectrometry (MS) rate among the most powerful technologies for comprehensive protein analysis. This article details the potential of both methods with regard to revealing methionine oxidation, a chemical modification that may be induced during downstream processing and storage of biopharmaceuticals. The benefits and limitations of bottom-up, middle-down, and top‑down HPLC–MS analysis will be demonstrated for the detection of oxidation variants in a therapeutic monoclonal antibody (mAb).
Latest:
How Analytics and Mass Spec Became the Driving Force Behind Biotherapeutic Drug DevelopmentThis article explores the key challenges analytics and mass spectrometry solve within the biopharmaceutical industry.
Latest:
Antibody–Drug Conjugates: Perspectives and CharacterizationThis instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.
Latest:
Antibody–Drug Conjugates: Perspectives and CharacterizationThis instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.
Latest:
Antibody–Drug Conjugates: Perspectives and CharacterizationThis instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.
Latest:
Antibody–Drug Conjugates: Perspectives and CharacterizationThis instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.
Latest:
Standardized Testing of Silica as a Base Material for Difficult Bonded-Phase Preparative ApplicationsThis year's symposium was held in New Orleans, Louisiana, USA. In this review, Ron Majors covers HPLC and UHPLC column and sample preparation highlights, summarizes the awards presented, and reviews the overall liquid-phase chromatographic trends.
Latest:
Solvents: An Overlooked Ally for Liquid Chromatography–Mass SpectrometryLiquid chromatography–mass spectrometry (LC–MS) platforms are continually developing to offer improved sensitivity to meet the analytical demands of today’s laboratories. However, choosing an inappropriate solvent can significantly undermine the quality of results, even when using the most advanced technology; a high-purity mobile phase with excellent batch-to-batch consistency is essential for reliable and reproducible results. This article discusses the importance of selecting the correct grade of solvent for LC–MS analyses and some of the challenges arising from an insufficiently pure mobile phase.
Latest:
Using GC to Investigate Nerve Agent Presence on Indoor SurfacesLCGC International recently spoke to Tomáš Rozsypal of the Nuclear, Biological and Chemical Defence Institute of the University of Defence (Vyskov, Czech Republic) about his work utilizing gas chromatography in researching the persistence of A-234 nerve agent on indoor surfaces, and the paper that resulted from it.
Latest:
A New Twist to Ion-Pairing Chromatography: In-Sample Addition of Ion-Pairing ReagentIon-pairing chromatography has traditionally been implemented with the addition of an ion-pairing reagent into the mobile phase. Here, an alternative method is presented, in which the ion-pairing reagent is deposited on column as a sample additive instead of in the mobile phase.
Latest:
Quantitative Comparison of Hormones in Drinking Water Between Low and High Resolution Mass SpectrometryThe quantitative performance of the latest generation of high-resolution instruments is comparable to that of a triple quadrupole MS, even though different scanning modes are used. Higher-resolution instrumentation also allows flexibility concerning compound identification because the experiment can be set up for targeted quantitation, screening, or both. In an Orbitrap-based instrument, the parallel reaction monitoring (PRM) mode performs most closely to a triple quadrupole mass analyzer using selected reaction monitoring (SRM) mode. This study looks at the performance of an Orbitrap-based LC–MS method for EPA Method 539.
Latest:
Solvent Selection in Countercurrent Chromatography Using Small-Volume Hydrostatic ColumnsHow to use small columns to test potential biphasic liquid systems for use in large-scale countercurrent chromatography separations
Latest:
Establishing Analytical & Functional Comparability for BiosimilarsThe authors discuss key findings from recent biosimilarity assessments they conducted on biosimilars of granulocyte-colony-stimulating factor (G-CSF), insulin glargine, rituximab, and trastuzumab.
Latest:
GC/GC–MSA snapshot of key trends and developments in the GC/GC–MS sector according to selected panelists from companies exhibiting at Analytica 2018.
Latest:
Current and Future Chromatographic Columns: Is One Column Enough to Rule Them All?The packed particle bed format still rules LC columns, but advances continue in monoliths. Meanwhile, newer formats are on the horizon, including microfabricated columns and 3D printed columns. This article provides a critical review of all these technologies and demonstrates how further development of chromatographic columns will be of paramount importance in the future.
Latest:
Current and Future Chromatographic Columns: Is One Column Enough to Rule Them All?The packed particle bed format still rules LC columns, but advances continue in monoliths. Meanwhile, newer formats are on the horizon, including microfabricated columns and 3D printed columns. This article provides a critical review of all these technologies and demonstrates how further development of chromatographic columns will be of paramount importance in the future.
Latest:
Hyphenated Techniques in Chromatography and Separation Technology (HTC-17) PreviewHTC-17 will be held in person from 18 to 20 May 2022, at The Aula in Ghent, Belgium. Here’s a taste of what to expect.
Latest:
Bioinert Versus Biocompatible: The Benefits of Different Column Materials in Liquid Chromatography SeparationsIn this study, we compare the performance of plastic and metal materials in UHPLC columns designed for the analysis of biological molecules. We evaluate the performance of these materials in terms of inertness, column chromatographic performance, and reproducibility.
Latest:
Systematic Evaluation of HILIC Stationary Phases for MS Characterization of OligonucleotidesHydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.
Latest:
The Use of HILIC Zwitterionic Phase Superficially Porous Particles for Metabolomics AnalysisThis article highlights the use of a new HILIC zwitterionic phase on superficially porous particles. A study on the use of a novel mobile-phase additive to achieve superior peak shape and isomer separation is also discussed, as well as improved LC–MS detection capabilities for metabolomics analysis.
Latest:
The Use of HILIC Zwitterionic Phase Superficially Porous Particles for Metabolomics AnalysisThis article highlights the use of a new HILIC zwitterionic phase on superficially porous particles. A study on the use of a novel mobile-phase additive to achieve superior peak shape and isomer separation is also discussed, as well as improved LC–MS detection capabilities for metabolomics analysis.
Latest:
Direct Liquid Chromatography Tandem Mass Spectrometry Analysis of Glyphosate, AMPA, Glufosinate, and MPPA in Water Without DerivatizationThis article describes a direct analysis of glyphosate, aminomethylphosphonic acid (AMPA), glufosinate, and 3-methylphosphinicopropionic acid (MPPA) in water by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) without derivatization. The chromatographic separation was performed using a hydrophilic interaction liquid chromatography (HILIC) column and typical LC–MS mobile phases. Method performance was evaluated, showing excellent results. The low limits of quantification (LLOQs) obtained meet the requirements of EU guidelines and could also be used to get an agreement in France where regulations require lower LLOQs (NOR: DEVL1703763V).
Latest:
Direct Liquid Chromatography Tandem Mass Spectrometry Analysis of Glyphosate, AMPA, Glufosinate, and MPPA in Water Without DerivatizationThis article describes a direct analysis of glyphosate, aminomethylphosphonic acid (AMPA), glufosinate, and 3-methylphosphinicopropionic acid (MPPA) in water by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) without derivatization. The chromatographic separation was performed using a hydrophilic interaction liquid chromatography (HILIC) column and typical LC–MS mobile phases. Method performance was evaluated, showing excellent results. The low limits of quantification (LLOQs) obtained meet the requirements of EU guidelines and could also be used to get an agreement in France where regulations require lower LLOQs (NOR: DEVL1703763V).
Latest:
Two-Dimensional Liquid Chromatography Does More Than Increase Peak CapacityTwo-dimensional liquid chromatography (2D-LC) is drawn out of the chromatographic toolbox if resolution for compounds of interest is insufficient. Recently, several studies have started to highlight 2D-LC as a tool of choice to streamline analytical workflows to increase automation making them less time-consuming. This article highlights two proven cases where 2D-LC does more than simply increase peak capacity.
Latest:
Application of Novel Balance Systems: Next Step Towards Laboratory Automatization?The key differences between a classical balance and currently available automated systems from the good weighing practice (GWP) perspective and within the scope of ISO9001:2015 quality standard are discussed. The systems under review are: (i) manual analytical balances; (ii) semi‑automatic systems; (iii) fully automatic systems; and (iv) integrated systems that use on-line coupling of the analytical balance with the instrument used for analytical measurements. The parameters defined in GWP guidelines, such as accuracy, uncertainty, minimum weight, and risks (including out‑of‑specification results), will be evaluated for all of these systems.
