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Enhancing the Sensitivity of Atmospheric Pressure Ionization Mass Spectrometry Using Flow Modulated Gas ChromatographyThe past decade has witnessed resurgent interest in coupling GC to atmospheric-pressure chemical ionization (APCI), which is suitable for the high column flows required for using flow modulation. This study assesses the use of GP-APCI with flow modulation for sensitive detection of selected trace organics.
Latest:
Enhancing the Sensitivity of Atmospheric Pressure Ionization Mass Spectrometry Using Flow Modulated Gas ChromatographyThe past decade has witnessed resurgent interest in coupling GC to atmospheric-pressure chemical ionization (APCI), which is suitable for the high column flows required for using flow modulation. This study assesses the use of GP-APCI with flow modulation for sensitive detection of selected trace organics.
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Determination of α-Amanitin in Human Serum by Solid-Phase Extracton Coupled with HPLC-UVA method is presented for the determination of ?-amanitin, a toxic cyclic octapeptide of amanitin mushrooms, in human serum by solid-phase extraction coupled with high performance liquid chromatography with UV detection.
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Perspectives on the Use of Retention Modeling to Streamline 2D-LC Method Development: Current State and Future ProspectsIf we are going to achieve widespread adoption of multidimensional separations, we need more systematic approaches to method development that rely less on user experience.
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A Fast, Robust, and Reliable Method for Sensitively Screening Drugs of Abuse in Human Urine for Forensic ToxicologyAnalyzing drugs of abuse (DoA) in human bodily fluids is crucial for clinical research and forensic toxicology. In these routine analyses, a large number of samples must be investigated, with a potentially high laboratory cost for each sample. As such, a reliable and affordable method is required for analysis. In this article, a fast, robust, and reliable method is presented for routine, high-throughput drug screening of urine samples.
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Evaluation of PGC Stationary Phases Under High-Temperature LC Conditions for the Analysis of Parabens in Food SamplesThis article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.
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Liquid Chromatography Methods for the Separation of Short RNA OligonucleotidesSynthetic oligonucleotides have become increasingly popular as a result of the recent discovery of ribonucleic acid interference (RNAi), a natural process for silencing gene expression.
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Supercritical Fluid Chromatography: A Workhorse in Drug DiscoveryIn this LCGC Blog, Daipayan Roy and Muhammed Qamar Farooq of Amgen discuss the history and impact of supercritical fluid chromatography (SFC) throughout the years.
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Frontiers in Ultrafast Chiral ChromatographyWe explore the developments that have made it possible to produce sub-minute, and, more recently, even sub-second enantiomeric separations. We also look at the questions that remain unanswered.
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In Search of The Needle in The Mariana Trench: Host Cell Proteins and the Problem of Dynamic RangeThis article explores the analytical challenges associated with HCP monitoring and reviews recent advances in HCP characterization, with special emphasis on high performance liquid chromatography mass spectrometry (HPLC–MS)-based methods and HCP enrichment techniques.
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Monitoring of Oxidation in Biopharmaceuticals with Top-to-Bottom High Performance Liquid Chromatography–Mass Spectrometry Methodologies: A Critical CheckFederal regulations concerning the safety and efficacy of biopharmaceuticals require the implementation of a comprehensive toolbox of physicochemical and biological characterization methods. In order to demonstrate consistent overall structure, even minute differences in primary structure and post‑translational modifications (PTMs) have to be detectable in therapeutic proteins. Because of their remarkable capability of revealing small changes in molecular structure, high performance liquid chromatography (HPLC) and mass spectrometry (MS) rate among the most powerful technologies for comprehensive protein analysis. This article details the potential of both methods with regard to revealing methionine oxidation, a chemical modification that may be induced during downstream processing and storage of biopharmaceuticals. The benefits and limitations of bottom-up, middle-down, and top‑down HPLC–MS analysis will be demonstrated for the detection of oxidation variants in a therapeutic monoclonal antibody (mAb).
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How Analytics and Mass Spec Became the Driving Force Behind Biotherapeutic Drug DevelopmentThis article explores the key challenges analytics and mass spectrometry solve within the biopharmaceutical industry.
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Antibody–Drug Conjugates: Perspectives and CharacterizationThis instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.
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Antibody–Drug Conjugates: Perspectives and CharacterizationThis instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.
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Antibody–Drug Conjugates: Perspectives and CharacterizationThis instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.
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Antibody–Drug Conjugates: Perspectives and CharacterizationThis instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.
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Standardized Testing of Silica as a Base Material for Difficult Bonded-Phase Preparative ApplicationsThis year's symposium was held in New Orleans, Louisiana, USA. In this review, Ron Majors covers HPLC and UHPLC column and sample preparation highlights, summarizes the awards presented, and reviews the overall liquid-phase chromatographic trends.
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Solvents: An Overlooked Ally for Liquid Chromatography–Mass SpectrometryLiquid chromatography–mass spectrometry (LC–MS) platforms are continually developing to offer improved sensitivity to meet the analytical demands of today’s laboratories. However, choosing an inappropriate solvent can significantly undermine the quality of results, even when using the most advanced technology; a high-purity mobile phase with excellent batch-to-batch consistency is essential for reliable and reproducible results. This article discusses the importance of selecting the correct grade of solvent for LC–MS analyses and some of the challenges arising from an insufficiently pure mobile phase.
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Analyzing Blood Metabolite Characteristics in Recently Born Preterm Infants with Bronchopulmonary Dysplasia Using LC–MS/MSAfter collecting dried blood spots from preterm infants with gestational age (GA) of less than 32 weeks within 24 hours after birth, researchers used liquid chromatography-tandem mass spectrometry (LC–MS/MS) to perform targeted measurements of metabolites to identify differential metabolites in preterm infants with bronchopulmonary dysplasia and uncovering circulating biomarkers for the early prediction of this chronic lung disease.
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A New Twist to Ion-Pairing Chromatography: In-Sample Addition of Ion-Pairing ReagentIon-pairing chromatography has traditionally been implemented with the addition of an ion-pairing reagent into the mobile phase. Here, an alternative method is presented, in which the ion-pairing reagent is deposited on column as a sample additive instead of in the mobile phase.
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Quantitative Comparison of Hormones in Drinking Water Between Low and High Resolution Mass SpectrometryThe quantitative performance of the latest generation of high-resolution instruments is comparable to that of a triple quadrupole MS, even though different scanning modes are used. Higher-resolution instrumentation also allows flexibility concerning compound identification because the experiment can be set up for targeted quantitation, screening, or both. In an Orbitrap-based instrument, the parallel reaction monitoring (PRM) mode performs most closely to a triple quadrupole mass analyzer using selected reaction monitoring (SRM) mode. This study looks at the performance of an Orbitrap-based LC–MS method for EPA Method 539.
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Solvent Selection in Countercurrent Chromatography Using Small-Volume Hydrostatic ColumnsHow to use small columns to test potential biphasic liquid systems for use in large-scale countercurrent chromatography separations
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Establishing Analytical & Functional Comparability for BiosimilarsThe authors discuss key findings from recent biosimilarity assessments they conducted on biosimilars of granulocyte-colony-stimulating factor (G-CSF), insulin glargine, rituximab, and trastuzumab.
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GC/GC–MSA snapshot of key trends and developments in the GC/GC–MS sector according to selected panelists from companies exhibiting at Analytica 2018.
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Current and Future Chromatographic Columns: Is One Column Enough to Rule Them All?The packed particle bed format still rules LC columns, but advances continue in monoliths. Meanwhile, newer formats are on the horizon, including microfabricated columns and 3D printed columns. This article provides a critical review of all these technologies and demonstrates how further development of chromatographic columns will be of paramount importance in the future.
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Current and Future Chromatographic Columns: Is One Column Enough to Rule Them All?The packed particle bed format still rules LC columns, but advances continue in monoliths. Meanwhile, newer formats are on the horizon, including microfabricated columns and 3D printed columns. This article provides a critical review of all these technologies and demonstrates how further development of chromatographic columns will be of paramount importance in the future.
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Hyphenated Techniques in Chromatography and Separation Technology (HTC-17) PreviewHTC-17 will be held in person from 18 to 20 May 2022, at The Aula in Ghent, Belgium. Here’s a taste of what to expect.
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Bioinert Versus Biocompatible: The Benefits of Different Column Materials in Liquid Chromatography SeparationsIn this study, we compare the performance of plastic and metal materials in UHPLC columns designed for the analysis of biological molecules. We evaluate the performance of these materials in terms of inertness, column chromatographic performance, and reproducibility.
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Systematic Evaluation of HILIC Stationary Phases for MS Characterization of OligonucleotidesHydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.
