How to Achieve Good LC Separations From Bad Actors: Method Conditions and Parameters for Improving Analysis of Basic Compounds

Learn what to consider for improving peak shape for method development of basic compounds and peptides. The impact of pH, stationary phase, and mobile phase will be presented.

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Event Overview:

Developing LC methods for optimum performance of basic analytes can be challenging. The familiar struggles with adequate retention and tailing peak shape which impacts resolution and the ability to identify impurities as well as achieve baseline separation can be overcome with knowledge of the options that are available. This presentation will detail method parameters such as pH, stationary phase, mobile phase additives, choice of detector, and how they can be tailored for better performance for basic analytes and their impact on acids and neutrals. Examples will include separations of small molecule pharmaceuticals, drugs of abuse, and peptides to demonstrate how improvements can be achieved on these common basic analyte chromatography challenges.

Key Learning Objectives:

• Understand what options exist for improving basic peak shape and sample loadability
• Learn how to select the right combination of stationary phase and mobile phase conditions
• Know how method parameters impact the separation performance of basic analytes

Who Should Attend:

• Scientists developing LC and LCMS methods
• Lab Managers
• Chromatographers
• QA/QC Managers
• Anyone struggling to achieve optimal chromatography of basic compounds
  

Speakers:

Stephanie A. Schuster, Ph.D. Senior
Technical Support Scientist
Advanced Material Technology

Register Free: https://globalmeet.webcasts.com/starthere.jsp?ei=1693122&tp_key=eae2d76e68&sti=mjhwebad