The sensitivity and ruggedness of chiroptical detectors have improved considerably during the past decade. In this article, the authors examine more than 230 chiral compounds using the latest laser-based polarimetry detector for high performance liquid chromatography (HPLC). They also examine the relationship between optical rotation at the detector wavelength of 675 nm and the sodium D line. In addition, the authors consider the sensitivity, linear dynamic range, and effect of solvent composition on rotation and its general use as an HPLC detector for chiral compounds.
LCGC 17(10), 946–957 (1999)PLS-DA With Univariate Filtration Refines Discovery of Untargeted Metabolomics Data Using LC–MS
May 23rd 2024As opposed to targeted metabolomics which looks only at a predetermined set of metabolites or their pathways, an untargeted approach was preferred by this research team because of its unbiased yet still comprehensive nature.