HPLC Detection and Evaluation of Chiral Compounds with a Laser-Based Chiroptical Detector
The sensitivity and ruggedness of chiroptical detectors have improved considerably during the past decade. In this article, the authors examine more than 230 chiral compounds using the latest laser-based polarimetry detector for high performance liquid chromatography (HPLC). They also examine the relationship between optical rotation at the detector wavelength of 675 nm and the sodium D line. In addition, the authors consider the sensitivity, linear dynamic range, and effect of solvent composition on rotation and its general use as an HPLC detector for chiral compounds.
LCGC 17(10), 946–957 (1999)
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
