HPLC

Here, a new automated active pharmaceutical ingredient (API) extraction platform is integrated with online liquid chromatography (LC) to assess drug product potency. The platform dissolves the tablet coating (if present), disintegrates the tablet, and disperses and solubilizes the tablet for analysis.

Deviations from the expected pressure in modern LC systems (too low, too high, or fluctuating) can be diagnosed and more quickly resolved using the streamlined troubleshooting practices shown here.

In this month’s blog, we provide information about the Subdivision on Chromatography and Separations Chemistry (SCSC) of the Analytical Division of the American Chemical Society (ACS), sharing our main goals and introducing our newly appointed executive board members.

Method validation processes will need to be refined to better suit forensic laboratories.

An improved LC–MS/MS method that can rapidly detect fipronil, an insecticide harmful to human health, is presented here for chicken eggs, feed, and soil.

What leads to an asymmetric peak shape? Physiochemical phenomena can help chromatographers identify whether the cause of asymmetry has a chemical or physical origin.

In this series of blog posts, I’m going to explore how the challenges of adopting methods from the literature, or from internal or external clients, can often be made easier, and more enjoyable, by taking time for some detective work prior to even entering the laboratory.

The UHPLC–MS/MS method can accurately determine the presence of these illegal feed additives in swine tissues.

With a new free simulator, educators and students can perform virtual HPLC experiments under isocratic or gradient conditions applicable to real instrumentation and method development.

As the field moves toward routine use of pressures well above 400 bar, the effect of pressure on retention should not be overlooked.

Understanding the fundamentals of extraction methodologies is critical, and can enable chromatographers to find smart solutions to tackle the complex composition of different types of samples.

Are the days numbered for long linear runs of univariate data in separation science?

Three-dimensional (3D) printing shows promise for creating stationary phases with tailored geometries. We present a method that overcomes limitations of other approaches, and can produce columns with a feature resolution of 10 μm.

Improved analysis of pharmaceutical and natural medicine products requires advances in reversed-phase LC stationary phases. We examine two synthesized stationary phases with applicability in quality control and chiral separation for analysis of natural products.

In a continuing series on peak shapes, we focus on potential physical causes of asymmetry, including column packing, changes in the packed particle bed, and accumulation of debris in the column.

Professor Pavel Jandera passed away suddenly and unexpectedly on August 5, 2021. He was one of the most famous Czech analytical chemists. He was 77 years old.

GC and TLC methods are demonstrated for quantification of stigmasterol 3-O-β-D-glucopyranoside (S3G), the main active component in the herbal nutraceutical Balanites aegyptiaca, an antihyperglycemic in Egyptian folk medicine.

This concise yet comprehensive overview of sample preparation for bioanalysis looks at sample preparation fundamentals, best practices, and modern trends—all illustrated with a case study.

In this first installment in a series on the causes of peak asymmetry, we discuss basic concepts in peak shape, explore poor fluidic connections as a common cause of peak tailing, and explain what to do about it.

I’m very much a “big picture” type of thinker. By that, I mean unless I can understand all of the working parts of a problem and understand how they interact, I find it difficult to decide the best approach to figuring out how to solve the issue.

Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.

This 7-minute method enables easy analysis of bitter flavors in beer.

When selecting new candidates for multifaceted analytical separation science jobs, we rarely find an exact match. So what should we be looking for in job candidates?

At ChromTalks, experienced speakers shared mistakes made during their careers.

Technology improvements have reignited interest in open-tubular LC, which promises high efficiency, low backpressure, and more environmentally sustainable separations.

In the final article of this series on extracolumn dispersion, we look at elution mode, post-column flow splitting, and a free calculator to use during method development.

I do not believe in classic linear separations anymore—further, I think separations science is wildly underdeveloped and under-appreciated.

Per- and polyfluoroalkyl substances (PFAS) in surface water have become a major concern due to their persistency and toxicity. We recently spoke to Stefan Van Leeuwen and Bjorn Berendsen of Wageningen Food Safety Research (WFSR), in The Netherlands, about their novel research into non-targeted PFAS screening using an LC–HRMS method with fragment ion flagging.

This is the first article in a four-part series exploring the quantitative assessment of drugs and their metabolites in biological fluids (such as blood, plasma, and urine) and tissue homogenates using liquid chromatography–mass spectrometry (LC–MS).

The stochastic theory of chromatography allows one to connect mathematics to separation science in an intelligible form. We take a “walk” through the column at the level of an Ant-Man, where we can see that chromatography is mathematics and mathematics is chromatography!