October 29th 2024
Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.
The Potential for Portable Capillary Liquid Chromatography
June 2nd 2020Is the desired goal of “shrinking down” capillary liquid chromatography (LC) from large laboratory systems to accurate portable field instruments realistic? This article explores recent progress in the miniaturization of LC components-such as capillary LC columns, micro- and nano-flow pumps, detectors, and other essential system components-and the future outlook for operating capillary LC instruments in remote settings.
Biocompatible Microextraction Devices for Simple and Green Analysis of Complex Systems
June 2nd 2020Pretreatment of complex samples remains a key step in the analytical workflow, critically impacting the overall accuracy of results. Pretreatment methods have been a challenge for food, biofluids, and environmental samples. Here, the development and evolution of biocompatible solid-phase microextraction (bio-SPME) as a sample pretreatment method are discussed for use in liquid chromatography and direct mass spectrometry applications.
Recovering from a COVID-19 Shutdown: Tips and Tricks for Starting Up, Part I
June 1st 2020When you restart liquid chromatography (LC) instrumentation that was idle during the COVID-19 shutdown, you need to follow a systematic approach. Otherwise, problems may appear in days or weeks following startup.
Moore’s Law in Liquid Chromatography: An Overview of 50 Years of (U)HPLC
June 1st 2020This article quantifies the progress in speed and efficiency that has been made in the area of liquid chromatography (LC) over the past 50 years. After a decade of groundbreaking advances in the 1970s, the progress in chromatographic performance over the ensuing four decades (1980–now) follows Moore’s law relatively closely. This is characteristic of technological fields that are of an evolutionary nature (that is, driven by the need for better performance).
Next Generation of Chromatographic Columns and Systems: From Theories to Possible Future Practices
May 1st 2020A series of theoretical, visualization, and simulation tools that are used to improve the structure and chemistry of the next generation of liquid chromatography (LC) columns is briefly reviewed.
Selectivity in Reversed-Phase Liquid Chromatography: 20 Years of the Hydrophobic Subtraction Model
April 1st 2020The hydrophobic subtraction model has been very successful. Nevertheless, the accompanying public database, which has parameters for 750 commercially available columns, is an underutilized column characterization tool. Here is some guidance on how to use both the model and the free database.
New HPLC Systems and Related Products Introduced in 2019–2020: A Brief Review
April 1st 2020Our annual review of new high performance liquid chromatography and mass spectrometry instruments, chromatography data systems (CDS), and related products, including a summary of their significant features and user benefits.
Three-Dimensional Chiral HPLC Analysis of Extraterrestrial Amino Acids in Carbonaceous Chondrites
January 20th 2020Aogu Furusho from the Graduate School of Pharmaceutical Sciences in Kyushu University, Fukuoka, Japan, spoke to us about his development of a highly selective three-dimensional chiral high performance liquid chromatography (HPLC) system for the determination of extraterrestrial amino acids in carbonaceous chondrites.
Advanced Peak Processing to Reduce Efforts in Method Optimization
December 10th 2019Partial coelution of chromatographic peaks is an often-encountered issue in high performance liquid chromatography (HPLC) analysis, despite best efforts in method development and optimization. Even though there are several ways of integration of overlapping signals, accurate quantification of single compounds using conventional photodiode array (PDA) detection is almost impossible without baseline separation. While extensive signal processing is well established in spectroscopic analyses such as infrared (IR) or nuclear magnetic resonance (NMR), it has not yet been commonly adapted to improve chromatographic data evaluation. This article introduces the theory and application of a novel data analysis technique for PDA detection to accurately determine and quantify single compounds, even from overlapping peaks, without the need for mass spectrometry (MS) detection.
Effects of Sample Diluent on Analyte Recovery in Reversed-Phase and HILIC Separations
September 1st 2019Carefully diluting a sample with weak solvent can mitigate the impact of sample solvent on peak shape in both reversed-phase and HILIC separations, but we need to understand how the choice of sample diluent can affect analyte recovery.
Evolution of Coupling Ion-Exchange Separations and Mass Spectrometry
August 1st 2019The high levels of non-volatile salts typically used in ion-exchange separations are incompatible with mass spectrometry. But the desire to directly couple these two powerful techniques has prompted researchers find solutions to this problem. Here are three approaches.
Understanding How Dwell Volume Can Affect Selectivity in Reversed-Phase Gradient Chromatography
June 1st 2019The effect of dwell volume on chromatographic selectivity can be successfully modelled using retention prediction software. Hence, the robustness of reversed-phase LC gradient methodologies, with respect to dwell volume, can be conveniently assessed.
Ultrahigh efficiency separations based on the presence of one deuterium in benzene, toluene, and naphthalene were achieved by recycle chromatography using C18 silica columns. The discrimination mechanism of H/D isotopic species is discussed based on the dispersion interactions of a CH/CD group of the solute with the stationary phase as well as the mobile phase.
Optimizing MS-Compatible Mobile Phases for IEX Separation of Monoclonal Antibodies
The impact of ionic strength, buffer capacity, and pH-response on the retention behavior and peak shape of mAb species characterization is evaluated for IEX-MS. The aim of the present study was to understand the impact of ionic strength, buffer capacity, and pH-response on the retention behavior and peak shape of mAb species.