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Analytical Tools for Enabling Process Analytical Technology: HPLC and Quantitative Polymerase Chain ReactionHow to use these tools to develop an efficient and robust process
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Recent Progress in Chiral Stationary Phase Development and Current Chiral ApplicationsA review of chiral separations, which remain a decided area of interest, particularly in the pharmaceutical and agrochemical fields.
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Static Headspace GC–MS Detection of Residual Solvents—A Possible Simultaneous Identification and Quantitation MethodA new procedure is proposed that provides identity parameters for headspace-applicable residual solvent Class 1 and Class 2 compounds addressed in the current US Pharmacopeia <467> method.
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Direct Insertion Probe – Mass Spectrometry in the Characterization of Opportunity CrudesDIP–MS is a fast and easy tool that can identify classes of compounds in opportunity crudes (heavy and ultraheavy crude oils, asphaltenes, and tar sands) in the field, without prior separation or treatment. It may enable fast screening of real samples to make a rough evaluation of the potential of reservoirs and oil fields.
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QuEChERS Sample Preparation for LC–MS-MS Determination of Avermectins in Meat and MilkWaters Corporation
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Coupling SFC with ELSD: Study of the Parameters Ruling ResponseThe article investigates the ELSD response variation with supercritical carbon dioxide-based mobile phases and compares the response with HPLC.
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Static Headspace GC–MS Detection of Residual Solvents—A Possible Simultaneous Identification and Quantitation MethodA new procedure is proposed that provides identity parameters for headspace-applicable residual solvent Class 1 and Class 2 compounds addressed in the current US Pharmacopeia <467> method.
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Astaxanthin from Shrimp By-products for Active Packaging SystemsThe two year international project "Preparation of active packaging with antioxidant and antimicrobial activity based on astaxanthin and chitosan",1 which started in 2009, was funded by FONCYCIT (Fund for International Cooperation of Science and Technology between the European Union and Mexico).
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Food Taints and Flavours — An Investigative ApproachA review of the analytical methods available to chromatographers for the determination of sources of off-flavours and food taints.
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Increased laboratory efficiency with the TSQ 9610 GC-MS/MS in a food and environmental analytical laboratoryHear how Eurofins SOFIA GmbH successfully implemented the TSQ 9610 GC-MS/MS into their workflow for trac contaminant analysis and increased higher sample throughput using the combination of instrumentations and software.
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Speciation Analysis of Mercury in Sediments by HPLC Using HPLC Hyphenated to Vapour Generation Atomic Fluorescence Spectrometry Following Microwave-Assisted ExtractionA novel method for analysing mercury in sediment is described.
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Desorption and Ionization Mass Spectrometry in Research LaboratoriesThe past decade has brought exponential growth in the number of mass spectrometry (MS) ionization techniques based on desorption and ionization (DI) processes. Here, the three key applications for DI are discussed: rapid, in situ screening; direct analysis of extracted samples or of planar chromatography spots; and scanning samples along x and y axes.
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Using Multiple Analytical Techniques in Non-Targeted Analysis of Food ProductsTraditional approaches for measuring food components are based on a ?target list? approach, whereby specific methodologies are developed for individual or related compounds. Such methods are well-suited for routine monitoring but can fail to detect emerging contamination events or to identify new prospects for product development.
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The Importance of Complete Overlapping of Analyte and Internal Standard Peaks in Eliminating Matrix Effects with Liquid Chromatography–Mass Spectrometry (LC–MS)Ensuring complete overlapping (coelution) of analyte and internal standard peaks can be critical for eliminating matrix effects in LC–MS/MS analysis.
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Perfecting Processes in Drug DiscoveryJanet Kelsey of The Column spoke to Frédéric Cheviron, Sales & Marketing Manager for Chiral Technologies Europe, about the technology they use in drug development and how companies can improve their method development.
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Assay for ppm Levels of Azide in Drug SubstancesThe removal of azide as a potential impurity from a drug substance may be critical to its safety profile. The quantitation of this impurity is an important control parameter. This article describes a simple and practical assay for azide using chemical derivatization and HPLC. The method is shown to be suitable for the intended purpose on three example test materials. Potential issues for wider applications are discussed.
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The Rise of Hydrophilic Interaction Chromatography in Untargeted Clinical MetabolomicsHydrophilic interaction chromatography (HILIC) was introduced more than two decades ago and has garnered much attention. Characterized by a hydrophilic stationary phase used in combination with an aqueous organic mobile phase, numerous improvements have been achieved and HILIC is now considered as an attractive alternative to reversed-phase phase liquid chromatography (LC) for many applications. HILIC provides several advantages over reversed-phase LC for the analysis of polar compounds, including higher retention of polar metabolites, enhanced mass spectrometric sensitivity, moderate back-pressure - even at high flow rates, or when used with sub-2-µm particle size - and orthogonal selectivity. Several important technical developments have been proposed during the last decade that foster its use in metabolomics. This review presents an overview of the most recent technical improvements and applications of HILIC analysis in untargeted clinical metabolomics and discusses important practical considerations, including the selection of the optimal column chemistry, appropriate eluents, sample preparation, and data analysis.
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Quality Control Methodologies for Pharmaceutical CounterionsQuantitative determination of the counterions associated with pharmaceutical salts is a mandatory requirement for quality control. While ion chromatography (IC) is the standard technique in most laboratories, capable of delivering excellent sensitivity, specificity and flexibility, there are other simpler and quicker analytical methodologies that may should be considered for this quality control application.
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Determination of Genotoxic Impurities in PharmaceuticalsThe determination of genotoxic impurities (GIs) in drug substances and pharmaceutical products is an emerging topic in pharmaceutical quality control. GIs are intermediates or reactants in the synthetic pathway of a drug substance and should be monitored at ppm (?g/g drug substance) or even ppb (ng/g) levels. This is several orders of magnitude lower than in classical impurity analysis (0.05% or 500 ppm level) or in residual solvent analysis. Analytical methods for the determination of GIs include gas chromatography (GC) and liquid chromatography (LC), both often combined with mass spectrometry (MS) detection. Some typical examples of GIs trace analysis using GC and LC are presented. The potential of on-line reaction monitoring is also discussed.
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Screening of Pollutants in Water Samples and Extracts from Passive Samples and Extracts from Passive Samplers Using LC–MS and GC–MSThis article describes the GC–MS and LC–MS screening methods developed for the analysis of both low-volume water samples and extracts obtained from various designs of passive samplers.
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Monitoring Water Framework Directive Priority Substances in WastewaterThe level of pollutants present in water bodies is most commonly judged against set environmental quality standards (EQSs) that vary among different countries.
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Hollow-Fibre Liquid-Phase Microextraction in the Three-Phase Mode — Practical ConsiderationsThis month's "Sample Prepartion Perspectives" describes practical aspects of hollow-fibre liquid-phase microextraction in the three phase mode (HF3LPME) and also electromembrane extraction (EME), which uses an electrical potential as the driving force for the extraction.
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Astaxanthin from Shrimp By-products for Active Packaging SystemsThe two year international project "Preparation of active packaging with antioxidant and antimicrobial activity based on astaxanthin and chitosan",1 which started in 2009, was funded by FONCYCIT (Fund for International Cooperation of Science and Technology between the European Union and Mexico).
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Purification and Isolation of Cannabinoids: Current Challenges and PerspectivesThe current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.
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A Novel Approach to Measure Crop Plant Protein ExpressionCrop development to improve yield or disease resistance has been explored for centuries and the technologies to measure these improvements have subsequently become complex.
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Static Headspace GC–MS Detection of Residual Solvents—A Possible Simultaneous Identification and Quantitation MethodA new procedure is proposed that provides identity parameters for headspace-applicable residual solvent Class 1 and Class 2 compounds addressed in the current US Pharmacopeia <467> method.
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Accurate Mass: Why It's the Best Solution for Metabolite Identification in Discovery, Development, and Clinical ApplicationsAccurate-mass approaches offer a significant advance over nominal-mass approaches in the arena of qualitative analysis, and some of the analytical approaches can now be conducted in a relatively routine manner.
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Variable Selection and Reduction in Multivariate Calibration and ModellingIn multivariate calibration and modelling a model is built between a large data matrix containing many variables for each sample and the property of the sample.
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Interactive Liquid Chromatography of Olefin-based ElastomersThe use of interactive liquid chromatography using porous graphite as a stationary phase and gradients of long chain alcohols and chlorinated aromatics to separate olefin-based elastomers according to their chemical composition is discussed.
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Next-Generation Stationary Phases: Properties and Performance of Core–Shell ColumnsThis short review focuses on the physicochemical properties of core–shell particles and their resulting chromatographic efficiencies as they relate to the comparison of their mass transfer kinetics to those of columns packed with sub-2-?m fully porous particles.
