Use of Synthetic Peptides to Determine Relative Retention Times for Automated Targeted Protein/Peptide Quantification Method Development
The Column
Protein/peptide discovery data obtained from data-dependent experiments have been used to increase the success rate of targeted quantification assays as well as automate method building.
Protein/peptide discovery data obtained from data-dependent experiments have been used to increase the success rate of targeted quantification assays as well as automate method building. The incorporation of a quality control (QC) peptide set for discovery experiments aids the determination of relative retention times, which are stored in spectral library entries with precursor and product ion information. The inclusion of both liquid chromatography (LC) and mass spectrometry (MS) data in spectral library entries enables software programs to accurately build targeted peptide acquisition methods for large lists of peptides. The efficiency of determining the relative retention times for a set of synthetic peptides was evaluated across a number of LC–MS methods.
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
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