Use of Synthetic Peptides to Determine Relative Retention Times for Automated Targeted Protein/Peptide Quantification Method Development
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Protein/peptide discovery data obtained from data-dependent experiments have been used to increase the success rate of targeted quantification assays as well as automate method building.
Protein/peptide discovery data obtained from data-dependent experiments have been used to increase the success rate of targeted quantification assays as well as automate method building. The incorporation of a quality control (QC) peptide set for discovery experiments aids the determination of relative retention times, which are stored in spectral library entries with precursor and product ion information. The inclusion of both liquid chromatography (LC) and mass spectrometry (MS) data in spectral library entries enables software programs to accurately build targeted peptide acquisition methods for large lists of peptides. The efficiency of determining the relative retention times for a set of synthetic peptides was evaluated across a number of LC–MS methods.
Polysorbate Quantification and Degradation Analysis via LC and Charged Aerosol Detection
April 9th 2025Scientists from ThermoFisher Scientific published a review article in the Journal of Chromatography A that provided an overview of HPLC analysis using charged aerosol detection can help with polysorbate quantification.
Analyzing Vitamin K1 Levels in Vegetables Eaten by Warfarin Patients Using HPLC UV–vis
April 9th 2025Research conducted by the Universitas Padjadjaran (Sumedang, Indonesia) focused on the measurement of vitamin K1 in various vegetables (specifically lettuce, cabbage, napa cabbage, and spinach) that were ingested by patients using warfarin. High performance liquid chromatography (HPLC) equipped with an ultraviolet detector set at 245 nm was used as the analytical technique.
Removing Double-Stranded RNA Impurities Using Chromatography
April 8th 2025Researchers from Agency for Science, Technology and Research in Singapore recently published a review article exploring how chromatography can be used to remove double-stranded RNA impurities during mRNA therapeutics production.
