A brief historical overview of DMS, followed by a synopsis of the instrumentation, physics, and chemistry behind the separation principles
This article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry.
Analysis of paralytic shellfish toxins according to the official AOAC Method 2011.02 using a HPLC single step-gradient method with products of post-column derivatization detected using a fluorescence detector.
The design of strategies to reduce the activity of residual silanols in conventional alkyl-bonded columns is still one of the main challenges in the current separation of basic compounds by reversed-phase liquid chromatography.
A new procedure is proposed that provides identity parameters for headspace-applicable residual solvent Class 1 and Class 2 compounds addressed in the current US Pharmacopeia <467> method.
KNAUER Application Note
In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
The article investigates the ELSD response variation with supercritical carbon dioxide-based mobile phases and compares the response with HPLC.
The use of interactive liquid chromatography using porous graphite as a stationary phase and gradients of long chain alcohols and chlorinated aromatics to separate olefin-based elastomers according to their chemical composition is discussed.
An update on the sample preparation and LC–MS-MS tools available for allergen detection, as well advantages of those techniques.
This article details a recent experiment that demonstrates how nonspecific interactions can be prevented by using metal-free or low adsorption flow paths.
Under a suitable thermal oxidation regime, vegetable oils yield a mixture of volatile and semivolatile organics that exhibit very high antimicrobial activities against a variety of microbial species. Volatile and semivolatile products were characterized with GC–MS using electron ionization and chemical ionization. The thermal oxidation of vegetable oils resulted in the formation of an array of short and medium-chain acids, aldehydes, and ketones that act synergistically to yield a potent antimicrobial disinfectant.
The level of pollutants present in water bodies is most commonly judged against set environmental quality standards (EQSs) that vary among different countries.
An overview of applications of various types of MS systems in drug discovery efforts, including in vitro and in vivo screening assays.
This is the second in a four-part series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, USA, from 19–24 June.
Describes the preparation of a reversed-phase monolithic column with an optimized porous structure to separate intact proteins using LC–MS.
Decision trees offer great visuals to observe complex data sets and to classify data according to simple decision rules.
Measuring trypsin inhibitors in legumes is important to feed processors, who are concerned with providing high-quality products for animal feed. In this study, a rapid, accurate, and precise method for the quantification of trypsin inhibitor activity is evaluated.
The main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
A look at the LC and LC–MS methods that are helping to overcome challenges in sample analysis, and how this can make adeno‑associated virus (AAV)–based gene therapy more accessible.
A systematic way to develop HPLC methods consists of building up and running a useful set of experiments (DOE, design of experiments) to evaluate the influence of several factors or method conditions on the selectivity of a chromatogram. The crucial part here is the peak tracking between the chromatograms.
In this third part in the series, we discuss chemical causes of peak asymmetry, including effects from mass overload and slow desorption kinetics.
Beverages, such as sodas and energy drinks, can include a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sugar substitutes), caffeine, vitamin supplements, amino acids, organic acids, and plant extracts. Because the analytes are already in solution, there is no need for extensive sample preparation. Dilution followed by direct injection into an HPLC is typically suitable.
A new procedure is proposed that provides identity parameters for headspace-applicable residual solvent Class 1 and Class 2 compounds addressed in the current US Pharmacopeia <467> method.
A novel method for analysing mercury in sediment is described.
We note here the passing of pioneering American gas chromatographer Walter G. Jennings on July 5, 2012. Walt, as he preferred, died peacefully at home surrounded by family. He was 90 years old.
The packed particle bed format still rules LC columns, but advances continue in monoliths. Meanwhile, newer formats are on the horizon, including microfabricated columns and 3D printed columns. This article provides a critical review of all these technologies and demonstrates how further development of chromatographic columns will be of paramount importance in the future.
The removal of azide as a potential impurity from a drug substance may be critical to its safety profile. The quantitation of this impurity is an important control parameter. This article describes a simple and practical assay for azide using chemical derivatization and HPLC. The method is shown to be suitable for the intended purpose on three example test materials. Potential issues for wider applications are discussed.