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Coupling Supercritical Fluid Chromatography with Evaporative Light Scattering Detection: A Study of the Relevant Parameters Ruling ResponseThe mobile-phase flow rate and the amount of modifier mixed with carbon dioxide are the main parameters affecting peak area.
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Toward Quantitative Imaging Mass SpectrometryThe main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
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Nomenclature and Conventions in Comprehensive Multidimensional Chromatography – An UpdateA review of nomenclature and conventions used in comprehensive multidimensional chromatography. An update of the popular paper on this topic from 2003.
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Speciation Analysis of Mercury in Sediments by HPLC Using HPLC Hyphenated to Vapour Generation Atomic Fluorescence Spectrometry Following Microwave-Assisted ExtractionA novel method for analysing mercury in sediment is described.
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Using Automated Cold-On-Column Injection for the Analysis of Explosive Residues by Negative Chemical Ionization GC-MSNegative chemical ionization GC-MS, used in conjunction with automated cold-on-column injection, provides efficient and sensitive quantification of explosive residues for environmental and forensic applications.
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Synthesis and Applications of BEH Particles in Liquid ChromatographyHow ethylene-bridged hybrid inorganic–organic (BEH) particles are prepared and how their special qualities can be used to improve separations
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Rapid, Efficient Quantification of Water in Solvents and Solvents in Water Using an Ionic Liquid–Based GC ColumnDeveloping versatile, rapid, and accurate analytical techniques for the detection and quantification of water in a variety of materials remains an important and ubiquitous analytical problem. This article describes an effective and sensitive ionic liquid (IL)-based capillary gas chromatography (GC) method with thermal conductivity detection (TCD) to determine the water content in liquid samples.
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Analysis of Terpenes in Cannabis Using Headspace Solid-Phase Microextraction and GC–MSAs the legalization of medicinal cannabis continues to sweep across the United States, an urgent need has developed for fast, accurate and efficient analytical testing. In addition to testing for contaminants and potency, there is also interest in the determination of terpene identity and concentration levels present in different strains of cannabis. Terpenes have been shown to have therapeutic uses for treatment of different medical conditions ranging from cancer and inflammation, to anxiety and sleeplessness. It is believed that the combination of terpenes and cannabinoids in cannabis produce a synergistic effect with regards to medical benefits. The traditional testing method for terpenes in plant materials involves a solvent-based extraction followed by GC analysis. In this work, headspace solid phase microextraction (HS-SPME) was used to identify and quantify terpene content in cannabis. The HS-SPME method provided several advantages over solvent extraction in that it provided a cleaner analysis, free of interferences from co-extracted matrix, and was non-destructive to the sample. A cannabis sample of unknown origin was first analyzed qualitatively by HS-SPME and GC-MS. Spectral library matching and retention indices were used to identify 42 different terpenes. Quantitative analysis was then performed for several selected terpenes using spiked samples. Method accuracy was >90%, with reproducibility of
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Polar Vapor-Enhanced Separations with Planar Differential Mobility Spectrometry-Mass SpectrometryA brief historical overview of DMS, followed by a synopsis of the instrumentation, physics, and chemistry behind the separation principles
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Host Cell Protein Monitoring During Downstream Processing Using Micro-Pillar Array Columns Combined with Mass SpectrometryThis article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry.
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Analysis of Paralytic Shellfish ToxinsAnalysis of paralytic shellfish toxins according to the official AOAC Method 2011.02 using a HPLC single step-gradient method with products of post-column derivatization detected using a fluorescence detector.
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Reliable Measurement of Silanol Suppression Potency in Alkyl-bonded Stationary PhasesThe design of strategies to reduce the activity of residual silanols in conventional alkyl-bonded columns is still one of the main challenges in the current separation of basic compounds by reversed-phase liquid chromatography.
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Static Headspace GC–MS Detection of Residual Solvents—A Possible Simultaneous Identification and Quantitation MethodA new procedure is proposed that provides identity parameters for headspace-applicable residual solvent Class 1 and Class 2 compounds addressed in the current US Pharmacopeia <467> method.
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Steviol Glycosides: Fast On-line SPE for Sample Cleaning of Plant ExtractsKNAUER Application Note
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Separation Science: The State of the Art: Field-Flow Fractionation: Extended Frontiers to Supramolecular and Complex Polymer MaterialsIn this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.
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Coupling SFC with ELSD: Study of the Parameters Ruling ResponseThe article investigates the ELSD response variation with supercritical carbon dioxide-based mobile phases and compares the response with HPLC.
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Interactive Liquid Chromatography of Olefin-based ElastomersThe use of interactive liquid chromatography using porous graphite as a stationary phase and gradients of long chain alcohols and chlorinated aromatics to separate olefin-based elastomers according to their chemical composition is discussed.
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Advanced Analysis of Allergens in Foods: LC–MS-MS Methodologies and Future InsightsAn update on the sample preparation and LC–MS-MS tools available for allergen detection, as well advantages of those techniques.
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Size-Exclusion Chromatography–Mass Spectrometry Analysis of mAbs on a Biocompatible Flow PathThis article details a recent experiment that demonstrates how nonspecific interactions can be prevented by using metal-free or low adsorption flow paths.
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Gas Chromatography–Mass Spectrometry Characterization of Vegetable Oil–Derived Potent Antimicrobial AgentsUnder a suitable thermal oxidation regime, vegetable oils yield a mixture of volatile and semivolatile organics that exhibit very high antimicrobial activities against a variety of microbial species. Volatile and semivolatile products were characterized with GC–MS using electron ionization and chemical ionization. The thermal oxidation of vegetable oils resulted in the formation of an array of short and medium-chain acids, aldehydes, and ketones that act synergistically to yield a potent antimicrobial disinfectant.
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Monitoring Water Framework Directive Priority Substances in WastewaterThe level of pollutants present in water bodies is most commonly judged against set environmental quality standards (EQSs) that vary among different countries.
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Mass Spectrometry: The Premier Analytical Tool for DMPK Scientists in a Drug Discovery EnvironmentAn overview of applications of various types of MS systems in drug discovery efforts, including in vitro and in vivo screening assays.
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Preview of Topics at HPLC 2016, Part 2: New Materials for UHPLC Analysis of Monoclonal Antibody Drugs and Antibody–Drug ConjugatesThis is the second in a four-part series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, USA, from 19–24 June.
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The Potential of Polymer Monolithic Capillary Columns for the LC–MS Analysis of Intact ProteinsDescribes the preparation of a reversed-phase monolithic column with an optimized porous structure to separate intact proteins using LC–MS.
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Statistics for Analysts Who Hate Statistics, Part VIII: Decision TreesDecision trees offer great visuals to observe complex data sets and to classify data according to simple decision rules.
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Rapid Quantification of Trypsin Inhibitors in Food and Feed Formulations with Electrospray Ionization Mass SpectrometryMeasuring trypsin inhibitors in legumes is important to feed processors, who are concerned with providing high-quality products for animal feed. In this study, a rapid, accurate, and precise method for the quantification of trypsin inhibitor activity is evaluated.
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Toward Quantitative Imaging Mass SpectrometryThe main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
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Applying LC and LC–MS Methods to the Characterization and Analysis of rAAVA look at the LC and LC–MS methods that are helping to overcome challenges in sample analysis, and how this can make adeno‑associated virus (AAV)–based gene therapy more accessible.
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Using MS Data for Peak Tracking in Computer Supported HPLC Method DevelopmentA systematic way to develop HPLC methods consists of building up and running a useful set of experiments (DOE, design of experiments) to evaluate the influence of several factors or method conditions on the selectivity of a chromatogram. The crucial part here is the peak tracking between the chromatograms.
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But My Peaks Are Not Gaussian! Part 3: Physicochemical Causes of Peak TailingIn this third part in the series, we discuss chemical causes of peak asymmetry, including effects from mass overload and slow desorption kinetics.
