Author
Latest:
Using Benzoyl Chloride Derivatization to Improve Small-Molecule Analysis in Biological Samples by LC–MS/MSIn metabolomics, LC–MS is a popular technique because of its ability to separate a wide range of metabolites, but the presence of many highly polar analytes in these samples poses a challenge. Benzoyl chloride derivatization can be a practical solution, improving both sensitivity and selectivity.
Latest:
Comparing the Chemical Profiles of Plant-Based and Traditional Meats Using GC–MS-Based MetabolomicsUntargeted GC–MS metabolomics with sample derivatization is shown here to be effective for measuring the chemical profiles of traditional and plant-based meat products.
Latest:
Gas Chromatography–Mass Spectrometry Characterization of Vegetable Oil–Derived Potent Antimicrobial AgentsUnder a suitable thermal oxidation regime, vegetable oils yield a mixture of volatile and semivolatile organics that exhibit very high antimicrobial activities against a variety of microbial species. Volatile and semivolatile products were characterized with GC–MS using electron ionization and chemical ionization. The thermal oxidation of vegetable oils resulted in the formation of an array of short and medium-chain acids, aldehydes, and ketones that act synergistically to yield a potent antimicrobial disinfectant.
Latest:
Gas Chromatography–Mass Spectrometry Characterization of Vegetable Oil–Derived Potent Antimicrobial AgentsUnder a suitable thermal oxidation regime, vegetable oils yield a mixture of volatile and semivolatile organics that exhibit very high antimicrobial activities against a variety of microbial species. Volatile and semivolatile products were characterized with GC–MS using electron ionization and chemical ionization. The thermal oxidation of vegetable oils resulted in the formation of an array of short and medium-chain acids, aldehydes, and ketones that act synergistically to yield a potent antimicrobial disinfectant.
Latest:
Mass Spectrometry Techniques to Unravel the Heterogeneity of GlycoproteinsSince glycans are responsible for bioactivity, solubility, immunogenicity, and clearance rate from circulation, it is vital to have a detailed map of glycans in therapeutic glycoproteins. Detailed glycoprotein structural analysis must be able to identify the peptide sequence where the glycans are attached as well as the structure of the glycan portion, including oligosaccharide sequence and glycosyl linkages. This article details methods for mass spectrometry experiments on both released glycans (“glycomics”), as well as on intact glycopeptides (“glycoproteomics”) using electron transfer dissociation, high-energy collision dissociation, and collision-induced dissociation fragmentation pathways, which are needed to fully elucidate the structure of glycoproteins.
Latest:
Evaluating Substance Use via Wastewater Analysis: An Overview of Analytical WorkflowsWastewater analysis has become an established approach for retrieving additional epidemiological information about the use of illicit drugs, alcohol, and tobacco at the population level. Here, we present an overview of the recent analytical frameworks and workflows for target and suspect analyses using low- and high-resolution mass spectrometry and discuss the latest advances in wastewater-based epidemiology (WBE).
Latest:
Evaluating Substance Use via Wastewater Analysis: An Overview of Analytical WorkflowsWastewater analysis has become an established approach for retrieving additional epidemiological information about the use of illicit drugs, alcohol, and tobacco at the population level. Here, we present an overview of the recent analytical frameworks and workflows for target and suspect analyses using low- and high-resolution mass spectrometry and discuss the latest advances in wastewater-based epidemiology (WBE).
Latest:
Evaluating Substance Use via Wastewater Analysis: An Overview of Analytical WorkflowsWastewater analysis has become an established approach for retrieving additional epidemiological information about the use of illicit drugs, alcohol, and tobacco at the population level. Here, we present an overview of the recent analytical frameworks and workflows for target and suspect analyses using low- and high-resolution mass spectrometry and discuss the latest advances in wastewater-based epidemiology (WBE).
Latest:
Haloacetic Acid Analysis Using Two-Dimensional Matrix-Elimination Ion ChromatographyThe disinfectants commonly used to treat public drinking water can react with naturally occurring organic and inorganic matter in the source water to form disinfection byproducts such as haloacetic acids. Here, we describe the use of two-dimensional matrix-elimination ion chromatography (MEIC) for haloacetic acid analysis. This method minimizes the impact of matrix ions.
Latest:
Column Pore Size—An Underutilized Variable for OptimizingIn advance of Pittcon 2018, leading scientists-Ronald Majors, Richard Henry, John W. Dolan, Zachary S. Breitbach, and Daniel W. Armstrong-who will be speaking at the LCGC awards symposium give us a preview of their talks.
Latest:
Analyzing Per- and Polyfluoroalkyl Substances in Drinking Water Using EPA Methods 533 and 537.1 with Semi-Automated Solid-Phase Extraction (SPE)There is a need for fast and reliable PFAS analyses to monitor drinking water quality. We report on the use of semi-automated sample preparation with EPA methods 533 and 537.1, assessing performance and processing time.
Latest:
Qualitative Analysis of CBD OilsAn HPLC method for the testing of CBD oils. The DLC-10 is a small footprint, easy-to-use and low-cost HPLC system. An isocratic assay offers reliability and ease of use.
Latest:
The LCGC Blog: Pesticide Analysis in Cannabis: Choosing the Right TechniqueThe presence of capable students and researchers, each with a technical niche to offer, prepares us for future opportunities. On top of that structure, we also aim to maintain this mindset for our instrument selection before beginning projects. The installment of core labs at UT Arlington (www.uta.edu/sirt), making a wide range of instruments available to all research groups, has allowed our students to brainstorm about which instrument is the most appropriate for a specific analysis rather than how to make a measurement work with a given instrument.
Latest:
Developments in Green ChromatographyWhy should you be concerned about mobile-phase degassing - it's all done automatically, isn't it?
Latest:
Application of Quality by Design Principles to a Pharmaceutical Sample Using UHPLC Method Development with Modeling TechnologiesA new type of method development that uses modeling to find the "best" separation for high performance liquid chromatography (HPLC) was investigated and principles of Quality by Design (QbD) were followed when planning the investigation. The process delivered precise results and the method was able be transferred to a routine quality control (QC) laboratory.
Latest:
The Benefits of Nano-Surface and Molecular‑Orientation Limited (nSMOL) Proteolysis for Monoclonal Antibody Drug AnalysisThe application of a novel approach that enables a selective proteolysis of the Fab region of monoclonal antibodies (mAbs) is described. The technique is called nano-surface and molecular-orientation limited (nSMOL) proteolysis. By restricting the proteolysis to the complementarity-determining region (CDR) of the mAb, there is considerable reduction in sample complexity and the time required for method set-up and optimization.
Latest:
PFAS Reference Material Optimisation on LC-HRAMSIn this poster, we explore parameters that could impact data integrity with the analytes in EPA Method 1633, UCMR 5 and the EU Drinking Water Directive regulations.
Latest:
Managing Heterogeneity with Incremental Sampling MethodologyIncremental sampling methodology laboratory processing is used to produce an appropriately sized subsample that has the analytes of interest at the same concentration as the large incremental sample collected in the field. The end goal is to produce results that represent the conditions at the site and facilitate good decisions.
Latest:
Practical Tools for LC Method TranslationWe discuss the theory, principles, and key equations required for method translation for both isocratic and gradient LC methods using the Avantor® ACE® Method Translator tool.
Latest:
LC Column Technology: The State of the ArtIn this extended special feature to celebrate the 30th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments. We asked key opinion leaders in the field to discuss the current state of the art in liquid chromatography column technology, gas chromatography, sample preparation, and liquid chromatography instruments. They also describe the latest practical developments in supercritical fluid chromatography, 3D printing, capillary electrophoresis, data handling, comprehensive two‑dimensional liquid chromatography, and multidimensional gas chromatography.
Latest:
In Bruges: HPLC 2025The 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025) will be held from Sunday through Thursday, June 15-19, 2025, in Bruges, Belgium.
Latest:
LC Instrumentation: The State of the ArtIn this extended special feature to celebrate the 30th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments. We asked key opinion leaders in the field to discuss the current state of the art in liquid chromatography instruments.
Latest:
EuSP2024 and GSAC2024 PreviewEuSP2024 | GSAC2024 — The 3rd European Sample Preparation Conference and 2nd Green and Sustainable Analytical Chemistry Conference will both take place from 15–18 September 2024 in Chania, Crete, Greece.
Latest:
Sample Preparation: The State of the ArtIn this extended special feature to celebrate the 30th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments. We asked key opinion leaders in the field to discuss the current state of the art in sample preparation.
Latest:
A New Era for Big Data and ChromatographyWe have entered a new stage in the era of accelerations. Moore’s law continues its expansion, increasing exponentially the computer power available. Other accelerations are remarkable, particularly easy access to cloud computing and the expansion and influence of artificial intelligence to practically all sectors of our society.
