Author


Alexander L.N. van Nuijs

Latest:

Evaluating Substance Use via Wastewater Analysis: An Overview of Analytical Workflows

Wastewater analysis has become an established approach for retrieving additional epidemiological information about the use of illicit drugs, alcohol, and tobacco at the population level. Here, we present an overview of the recent analytical frameworks and workflows for target and suspect analyses using low- and high-resolution mass spectrometry and discuss the latest advances in wastewater-based epidemiology (WBE).


Frederic Been

Latest:

Evaluating Substance Use via Wastewater Analysis: An Overview of Analytical Workflows

Wastewater analysis has become an established approach for retrieving additional epidemiological information about the use of illicit drugs, alcohol, and tobacco at the population level. Here, we present an overview of the recent analytical frameworks and workflows for target and suspect analyses using low- and high-resolution mass spectrometry and discuss the latest advances in wastewater-based epidemiology (WBE).


Herb Wagner

Latest:

Haloacetic Acid Analysis Using Two-Dimensional Matrix-Elimination Ion Chromatography

The disinfectants commonly used to treat public drinking water can react with naturally occurring organic and inorganic matter in the source water to form disinfection byproducts such as haloacetic acids. Here, we describe the use of two-dimensional matrix-elimination ion chromatography (MEIC) for haloacetic acid analysis. This method minimizes the impact of matrix ions.


Stephanie A. Schuster

Latest:

Column Pore Size—An Underutilized Variable for Optimizing

In advance of Pittcon 2018, leading scientists-Ronald Majors, Richard Henry, John W. Dolan, Zachary S. Breitbach, and Daniel W. Armstrong-who will be speaking at the LCGC awards symposium give us a preview of their talks.


Ruud Addink

Latest:

Analyzing Per- and Polyfluoroalkyl Substances in Drinking Water Using EPA Methods 533 and 537.1 with Semi-Automated Solid-Phase Extraction (SPE)

There is a need for fast and reliable PFAS analyses to monitor drinking water quality. We report on the use of semi-automated sample preparation with EPA methods 533 and 537.1, assessing performance and processing time.


D-Star Instruments, Inc.

Latest:

Qualitative Analysis of CBD Oils

An HPLC method for the testing of CBD oils. The DLC-10 is a small footprint, easy-to-use and low-cost HPLC system. An isocratic assay offers reliability and ease of use.


Doug D. Carlton, Jr.

Latest:

The LCGC Blog: Pesticide Analysis in Cannabis: Choosing the Right Technique

The presence of capable students and researchers, each with a technical niche to offer, prepares us for future opportunities. On top of that structure, we also aim to maintain this mindset for our instrument selection before beginning projects. The installment of core labs at UT Arlington (www.uta.edu/sirt), making a wide range of instruments available to all research groups, has allowed our students to brainstorm about which instrument is the most appropriate for a specific analysis rather than how to make a measurement work with a given instrument.



Marek Tobiszewski

Latest:

Developments in Green Chromatography

Why should you be concerned about mobile-phase degassing - it's all done automatically, isn't it?



R. Kormany

Latest:

Application of Quality by Design Principles to a Pharmaceutical Sample Using UHPLC Method Development with Modeling Technologies

A new type of method development that uses modeling to find the "best" separation for high performance liquid chromatography (HPLC) was investigated and principles of Quality by Design (QbD) were followed when planning the investigation. The process delivered precise results and the method was able be transferred to a routine quality control (QC) laboratory.


Neil Loftus

Latest:

The Benefits of Nano-Surface and Molecular‑Orientation Limited (nSMOL) Proteolysis for Monoclonal Antibody Drug Analysis

The application of a novel approach that enables a selective proteolysis of the Fab region of monoclonal antibodies (mAbs) is described. The technique is called nano-surface and molecular-orientation limited (nSMOL) proteolysis. By restricting the proteolysis to the complementarity-determining region (CDR) of the mAb, there is considerable reduction in sample complexity and the time required for method set-up and optimization.


LGC Standards

Latest:

PFAS Reference Material Optimisation on LC-HRAMS

In this poster, we explore parameters that could impact data integrity with the analytes in EPA Method 1633, UCMR 5 and the EU Drinking Water Directive regulations.


Mark Bruce

Latest:

Managing Heterogeneity with Incremental Sampling Methodology

Incremental sampling methodology laboratory processing is used to produce an appropriately sized subsample that has the analytes of interest at the same concentration as the large incremental sample collected in the field. The end goal is to produce results that represent the conditions at the site and facilitate good decisions.


VWR International Ltd

Latest:

Practical Tools for LC Method Translation

We discuss the theory, principles, and key equations required for method translation for both isocratic and gradient LC methods using the Avantor® ACE® Method Translator tool.


Mel Euerby

Latest:

LC Column Technology: The State of the Art

In this extended special feature to celebrate the 30th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments. We asked key opinion leaders in the field to discuss the current state of the art in liquid chromatography column technology, gas chromatography, sample preparation, and liquid chromatography instruments. They also describe the latest practical developments in supercritical fluid chromatography, 3D printing, capillary electrophoresis, data handling, comprehensive two‑dimensional liquid chromatography, and multidimensional gas chromatography.


Frédéric Lynen

Latest:

Possibilities and Limitations of Aqueous Temperature Responsive Liquid Chromatography in Comprehensive 2D–LC

Analyzing representative standard mixtures, APIs, and synthetic impurities shows that when TRLC is combined with RPLC in 2D-LC, separation performance is improved. We explain why.


Sabine Heinisch

Latest:

LC Instrumentation: The State of the Art

In this extended special feature to celebrate the 30th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments. We asked key opinion leaders in the field to discuss the current state of the art in liquid chromatography instruments.


Elia Psillakis

Latest:

EuSP2024 and GSAC2024 Preview

EuSP2024 | GSAC2024 — The 3rd European Sample Preparation Conference and 2nd Green and Sustainable Analytical Chemistry Conference will both take place from 15–18 September 2024 in Chania, Crete, Greece.


David Benanou

Latest:

Sample Preparation: The State of the Art

In this extended special feature to celebrate the 30th anniversary edition of LCGC Europe, leading figures from the separation science community explore contemporary trends in separation science and identify possible future developments. We asked key opinion leaders in the field to discuss the current state of the art in sample preparation.


Gabriel Vivó-Truyols

Latest:

A New Era for Big Data and Chromatography

We have entered a new stage in the era of accelerations. Moore’s law continues its expansion, increasing exponentially the computer power available. Other accelerations are remarkable, particularly easy access to cloud computing and the expansion and influence of artificial intelligence to practically all sectors of our society.


Jelle De Vos

Latest:

Pushing Boundries

An introduction from our guest editors.






Darshan C. Patel

Latest:

Peak Shapes and Their Measurements: The Need and the Concept Behind Total Peak Shape Analysis

Gaussian peak shapes in chromatography are indicative of a well-behaved system. Such peak shapes are highly desirable from the perspective of column packing technology. From an analyst’s point of view, Gaussian peaks provide improved sensitivity (lower detection limits) and allow ease of quantitation. In practice, one can obtain peaks that tail, front, or concurrently front and tail for reasons such as column packing issues, chemical and kinetic effects, and suboptimal high performance liquid chromatography (HPLC) system plumbing and detector settings. Here, we discuss a number of approaches for peak shape measurement that are available in modern chromatography software, along with their advantages and drawbacks. A new “total peak shape analysis” approach is suggested that facilitates detection and quantification of concurrent fronting and tailing in peaks. Several remediation approaches are proposed that can help chromatographers analyze and improve peak shapes.


Mark E. Newton

Latest:

The Question of Chromatographic Integration in Data Integrity

The topic of chromatographic integration and data interpretation raises a lot of questions about data integrity. Is it acceptable to integrate data? What are the limits?


Ian John Edwards

Latest:

The Role of Mass Spectrometry in Biopharmaceutical Drug Discovery and Development

The discovery and development of biopharmaceuticals that target specific diseases can be transformative for people living with illness. However, bringing a new therapy to market is a prolonged and costly process mired in uncertainty. Ensuring safety, efficacy, and product quality is paramount. Biopharmaceuticals, by their nature, are highly complex. A myriad of heterogeneity can be intentionally functional, an unwanted consequence of manufacturing and storage, or generated by biological modification in vivo. Not all, but some post-translational modifications or biotransformations can impact development, manufacturing, safety, efficacy, and overall product quality. These critical quality attributes (CQAs) need to be identified, characterized, controlled, and monitored throughout the drug discovery and development cycle. Specialty measurement using mass spectrometry (MS) continues to play an ever‑increasing role across the continuum.


Bo An

Latest:

Efficient Quantitation of Biotherapeutics in Biological Matrices Through the Application of LC–MS‑Related Techniques

Biotherapeutics must endure in-depth testing to validate their efficacy and safety before their release to the medical community. Characterization and quantitation of these large molecule medicines is traditionally performed with ligand binding assays or radiolabeling procedures. Issues with selectivity, accuracy, and unavailability of applicable assays for the characterization and quantitation of certain biotherapeutics means that liquid chromatography–mass spectrometry (LC–MS) is becoming an increasingly selected method for biotherapeutics testing. Typically used for small molecules, LC–MS can be adapted for larger molecule analysis with additional high throughput and multiplexing capabilities. New method development has turned LC–MS into a highly sensitive option for biotherapeutics validation.