Author


J.R. Engen

Latest:

Applicability of Hydrogen–Deuterium Exchange in Comparing Conformations of Innovator to Biosimilar Biopharmaceutical Products

Significant recent advances now enable routine usage of HDX-MS for comparing the conformations of biopharmaceutical products.



Katrien Welvaert

Latest:

Enhanced Sample Throughput for Environmental Analysis

As environmental legislation becomes more stringent, the need to deliver quantitative results in shorter times and greater volumes is necessary for routine environmental analysis. Most of the high-throughput screening methods used to analyze pharmaceutical compounds are, however, useless for environmental monitoring. This is because these methods primarily aim to retrieve as much information from a single sample using the broadest range of techniques. The chromatographic separation process is considered to be the bottleneck in the process. This is not the situation for environmental procedures, in which the bottleneck is the sample preparation step and is usually very tedious and time-consuming.


Jeff Op De Beeck

Latest:

Host Cell Protein Monitoring During Downstream Processing Using Micro-Pillar Array Columns Combined with Mass Spectrometry

This article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry.


Bo Claerebout

Latest:

Peptide Mapping of Monoclonal Antibodies and Antibody–Drug Conjugates Using Micro-Pillar Array Columns Combined with Mass Spectrometry

The structural complexity of monoclonal antibodies (mAbs) challenges the capabilities of even the most advanced chromatography and mass spectrometry techniques. This study examines the use of micro-pillar array columns in combination with mass spectrometry for peptide mapping of both mAbs and antibody–drug conjugates (ADCs).


Jonathan Vandenbussche

Latest:

Host Cell Protein Monitoring During Downstream Processing Using Micro-Pillar Array Columns Combined with Mass Spectrometry

This article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry.


Elise Lemasson

Latest:

Mixed-Mode Chromatography—A Review

Mixed-mode high performance liquid chromatography (MM-HPLC) involves the combined use of two (or more) retention mechanisms in a single chromatographic system. Many original stationary phases have been proposed in recent years with promising possibilities, while applications have only started to appear in the literature. In this review, the authors discuss mixed-mode chromatography stationary phases. An overview of applications using mixed-mode chromatography is described, as well as the increased interest in mixed-mode systems for two-dimensional chromatography.


Philippe Hennig

Latest:

Mixed-Mode Chromatography—A Review

Mixed-mode high performance liquid chromatography (MM-HPLC) involves the combined use of two (or more) retention mechanisms in a single chromatographic system. Many original stationary phases have been proposed in recent years with promising possibilities, while applications have only started to appear in the literature. In this review, the authors discuss mixed-mode chromatography stationary phases. An overview of applications using mixed-mode chromatography is described, as well as the increased interest in mixed-mode systems for two-dimensional chromatography.


Sophie Bertin

Latest:

Mixed-Mode Chromatography—A Review

Mixed-mode high performance liquid chromatography (MM-HPLC) involves the combined use of two (or more) retention mechanisms in a single chromatographic system. Many original stationary phases have been proposed in recent years with promising possibilities, while applications have only started to appear in the literature. In this review, the authors discuss mixed-mode chromatography stationary phases. An overview of applications using mixed-mode chromatography is described, as well as the increased interest in mixed-mode systems for two-dimensional chromatography.


Ramon Soto Alvarez

Latest:

Rapid Identification of Illicit Drug Substances Using Thermal Desorption Coupled with a Portable Toroidal Trap GC–MS System

Recent developments in the miniaturization of gas chromatography–mass spectrometry (GC–MS) instrumentation are making the technique available for field-based investigations, offering a simple, onsite identification of drug substances. This article describes the identification of sixteen drugs compounds in less than 10 min using portable gas chromatograph-toroidal ion trap mass spectrometry combined with a coiled-wire-filament (CWF) sampling injector to provide an effective tool for the rapid analysis of illicit drug substances.


David C. Collins

Latest:

Rapid Identification of Illicit Drug Substances Using Thermal Desorption Coupled with a Portable Toroidal Trap GC–MS System

Recent developments in the miniaturization of gas chromatography–mass spectrometry (GC–MS) instrumentation are making the technique available for field-based investigations, offering a simple, onsite identification of drug substances. This article describes the identification of sixteen drugs compounds in less than 10 min using portable gas chromatograph-toroidal ion trap mass spectrometry combined with a coiled-wire-filament (CWF) sampling injector to provide an effective tool for the rapid analysis of illicit drug substances.


Ashley Thornock

Latest:

Rapid Identification of Illicit Drug Substances Using Thermal Desorption Coupled with a Portable Toroidal Trap GC–MS System

Recent developments in the miniaturization of gas chromatography–mass spectrometry (GC–MS) instrumentation are making the technique available for field-based investigations, offering a simple, onsite identification of drug substances. This article describes the identification of sixteen drugs compounds in less than 10 min using portable gas chromatograph-toroidal ion trap mass spectrometry combined with a coiled-wire-filament (CWF) sampling injector to provide an effective tool for the rapid analysis of illicit drug substances.


Serena Michalsky

Latest:

Rapid Identification of Illicit Drug Substances Using Thermal Desorption Coupled with a Portable Toroidal Trap GC–MS System

Recent developments in the miniaturization of gas chromatography–mass spectrometry (GC–MS) instrumentation are making the technique available for field-based investigations, offering a simple, onsite identification of drug substances. This article describes the identification of sixteen drugs compounds in less than 10 min using portable gas chromatograph-toroidal ion trap mass spectrometry combined with a coiled-wire-filament (CWF) sampling injector to provide an effective tool for the rapid analysis of illicit drug substances.


Stefan Neubauer

Latest:

Trends in Enantioselective High Performance Liquid Chromatography

Enantioselective high performance liquid chromatography (HPLC) is slowly adopting the modern particle technologies (sub-2-µm fully porous particles [FPPs] and sub-3-µm superficially porous silica particles [SPPs]) that have been well known in reversed-phase LC for the past decade. The most significant benefit is that enantiomer separations can be performed much faster, which is of interest in high-throughput screening applications and multidimensional enantioselective HPLC analysis. The state of the art is briefly discussed with some examples documenting the potential of core–shell particle technology and comprehensive multidimensional separations.


Ulrich Woiwode

Latest:

Trends in Enantioselective High Performance Liquid Chromatography

Enantioselective high performance liquid chromatography (HPLC) is slowly adopting the modern particle technologies (sub-2-µm fully porous particles [FPPs] and sub-3-µm superficially porous silica particles [SPPs]) that have been well known in reversed-phase LC for the past decade. The most significant benefit is that enantiomer separations can be performed much faster, which is of interest in high-throughput screening applications and multidimensional enantioselective HPLC analysis. The state of the art is briefly discussed with some examples documenting the potential of core–shell particle technology and comprehensive multidimensional separations.


Mike Kaupert

Latest:

Trends in Enantioselective High Performance Liquid Chromatography

Enantioselective high performance liquid chromatography (HPLC) is slowly adopting the modern particle technologies (sub-2-µm fully porous particles [FPPs] and sub-3-µm superficially porous silica particles [SPPs]) that have been well known in reversed-phase LC for the past decade. The most significant benefit is that enantiomer separations can be performed much faster, which is of interest in high-throughput screening applications and multidimensional enantioselective HPLC analysis. The state of the art is briefly discussed with some examples documenting the potential of core–shell particle technology and comprehensive multidimensional separations.


Scott Berger

Latest:

Mass Spectrometry in Late Development and QC: Practical Considerations for Multi-Attribute Monitoring and Beyond

As a result of the pharmaceutical cGMP for the 21st century and quality by design (QbD) initiatives championed by regulators, the biopharmaceutical industry has been looking for ways to introduce more automated and higher information content analyses into manufacturing, late-development, and quality control (QC). Mass spectrometry (MS-) based attribute monitoring assays have been proposed as key tools to provide the sensitivity, throughput, selectivity, and flexibility required for monitoring critical product and process attributes for biopharmaceutical production and release. Two analytical workflows, subunit multi-attribute monitoring (MAM) and peptide MAM, have emerged to dominate this discussion, and this article is intended to reflect on the active debates over the needs, challenges, and practical limitations for adopting MS-based attribute monitoring for late-development and QC.


Amos Heckendorf

Latest:

Electrostatic Repulsion–Hydrophilic Interaction Chromatography: Using One Mode to Tune Retention from a Second Mode

Electrostatic effects superimposed on hydrophilic interactions enables selection or deselection of functional groups for compound isolation.


A. Rathore

Latest:

Methods and Purposes for Determining Higher Order Structures of Biopharmaceuticals

Determining the higher order structure of a protein pharmaceutical is important. Here, we review the approaches for HOS determination that are currently receiving the most attention in the literature and at scientific meetings.


Parastoo Azadi

Latest:

Mass Spectrometry Techniques to Unravel the Heterogeneity of Glycoproteins

Since glycans are responsible for bioactivity, solubility, immunogenicity, and clearance rate from circulation, it is vital to have a detailed map of glycans in therapeutic glycoproteins. Detailed glycoprotein structural analysis must be able to identify the peptide sequence where the glycans are attached as well as the structure of the glycan portion, including oligosaccharide sequence and glycosyl linkages. This article details methods for mass spectrometry experiments on both released glycans (“glycomics”), as well as on intact glycopeptides (“glycoproteomics”) using electron transfer dissociation, high-energy collision dissociation, and collision-induced dissociation fragmentation pathways, which are needed to fully elucidate the structure of glycoproteins.



MilliporeSigma

Latest:

Merck: Four Ways to Better Water Quality with LC-MS

This article will discuss several aspects of working with high purity water in LC-MS analyses including quality and handling. Solutions will be proposed for optimal water purification based on the use of complementary purification technologies combined with application-specific final filtering, and finally, the importance of water purification system maintenance will be discussed.


Shodex, Showa Denko America, Inc.

Latest:

LC/MS Analysis of Neurotransmitters

Here we introduce LC/MS analysis methods using the Shodex™ HILICpak™ VC-50 2D column for various cationic neurotransmitters.


Joachim Ermer

Latest:

Why System Suitability Tests Are Not a Substitute for Analytical Instrument Qualification (Part 3): Performance Qualification (PQ)

A look at the role of system suitability tests (SSTs) during performance qualification (PQ).


Elizabeth M. Humston-Fulmer

Latest:

Detection and Characterization of Extractables in Food Packaging Materials by GC–MS

In this study, general extract screening of food storage materials was done with nontargeted analytical methods to understand what analytes could potentially leach into food or beverages. GC and mass spectral deconvolution effectively separated analytes within the complex mixture and TOF-MS provided full mass range spectral data for identification. This workflow can be used for confident characterization of components present as extractables from food packaging materials.


Urszula Gaik

Latest:

Ion Mobility Spectrometers as Chromatographic Detectors

Interest in connecting ion mobility spectrometry (IMS) to GC and especially to LC is now growing. One favorable property of IMS is that it can work with ambient pressure and can be easily connected to a gas or liquid chromatograph. Analytical applications of GC–MS and LC–MS are very different and encompass investigations into food, medical science, environment, drugs of abuse, chemical warfare agents, and explosives.


Edyta Budzy&#324ska

Latest:

Ion Mobility Spectrometers as Chromatographic Detectors

Interest in connecting ion mobility spectrometry (IMS) to GC and especially to LC is now growing. One favorable property of IMS is that it can work with ambient pressure and can be easily connected to a gas or liquid chromatograph. Analytical applications of GC–MS and LC–MS are very different and encompass investigations into food, medical science, environment, drugs of abuse, chemical warfare agents, and explosives.


DPX Labs

Latest:

Simultaneous, Automated Extraction of 96 Urine Samples for Drugs of Abuse Analysis by LC–MS/MS Using the Microlab® NIMBUS® Workstation

Utilizing Hamilton’s CO-RE® disposable tips with DPX technology provides a fast, accurate, and simple extraction method for analyzing drugs of abuse in urine. The Microlab NIMBUS equipped with a CO-RE 96-channel Multi-Probe Head (MPH) allows for high-throughput, automated sample processing.


ESC Lab Sciences

Latest:

Validation of Horizon Technology Disk Extraction Technology for US EPA Wastewater Method 625.1

This application note will present the data collected as part of the demonstration of disk solid phase extraction validation for US EPA method 625.1.


Jon W. Wong

Latest:

Important Considerations Regarding Matrix Effects When Developing Reliable Analytical Residue Methods Using Mass Spectrometry

Practical examples of how to correct for matrix effects in food testing to obtain reliable quantitative data using LC–MS and GC–MS