December 20th 2024
This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
DEA Acquires Waters Instrument for Training Forensic Chemists
June 1st 2009Waters Corporation (Milford, Massachusetts) announced that the U.S. Drug Enforcement Administration has purchased a Waters ACQUITY UltraPerformance liquid chromatograph for use in training new forensic chemists at the agency's Special Testing and Research Laboratory in Dulles, Virginia.
Agilent Technologies Partners with China Environmental Center to Detect Toxins in Water and Soil
June 1st 2009Agilent Technologies, Inc. (Santa Clara, California) announced it has jointly developed a method to detect and identify perfluorinated compounds (PFCs) in water and soil using high-performance liquid chromatography? mass spectrometry (HPLC?MS) with the China National Environmental Monitoring Center (CNEMC).
Novel Analytical Methods for the Discovery and Trace Analysis of Biochemically Active Compounds
June 1st 2009This article will discusss novel analytical methods for the discovery and trace analysis of biochemically active compunds in three main areas: protein analysis, screening technologies and multidimensional separations. The research in protein analysis describes analytical methods that involve automation and integration of sample clean-up, followed by separation and detection of the peptides/proteins.
LC-MS Study Shows Reliable way to Measure Leukemia Markers
May 1st 2009A study led by cancer researchers at Ohio State University's Comprehensive Cancer Center's Experimental Therapeutics Program.has validated a method for reliably measuring variations in certain proteins that may make good biomarkers in chronic leukemia patients.
Analysis of Genotoxic Impurities
May 1st 2009Researchers from the Research Institute for Chromatography (Kortrijk, Belgium) and AstraZeneca R&D Charnwood (Loughborough, UK) used in-situ derivatization and liquid chromatography?mass spectrometry (LC?MS) to analyze arylamines and aminopyridines, which can be present as potentially genotoxic impurities in pharmaceutical preparations at trace levels.
Agilent Partners with University of Michigan for Prostate Cancer Research
April 15th 2009Agilent Technologies (Santa Clara, California) announced its support of prostate cancer research by the University of Michigan?s (Ann Arbor) Center for Translational Pathology through a grant of instruments and funding.
Approaches to Singleton Achiral Purification of Difficult Samples for Discovery Research Support
April 1st 2009Several approaches for purifying difficult samples more efficiently for discovery research support are mentioned in this paper. These approaches use mass triggered HPLC on various specialty columns.
Sulfated β-Cyclodextrin as Chiral Mobile Phase Additive for Ultrahigh-Pressure Liquid Chromatography
A fast enantiomeric separation of a chiral aromatic amine was achieved, using ultra high pressure liquid chromatography and highly sulfated β-cyclodextrin (S-β-CD) as a chiral additive in the mobile phase. The stationary phase consisted of a core shell support with a particle size of 2.7 µm. Under these conditions the baseline separation was obtained within 2.5 min. The influence of the concentration of the additive, along with the thermodynamics of the separation, were studied. Molecular mechanics calculations were consistent with the experimental data for the order of elution, providing further evidence of these interactions. The enantiomeric separation at high temperature (90 °C) using only water as mobile phase also was achieved for the first time.
Superficially Porous and Monolithic Columns for Ultrafast Separations in HPLC
April 1st 2009Due to economic crisis all over the world it is a time of cost-friendly analyses. Superficially porous and monolithic columns are the tools to serve this purpose. These columns are new generation and can be used for ultrafast separations. This article describes the state-of-the-art for these stationary phases for high performance liquid chromatography (HPLC). The emphasis has been placed on their preparation, properties, applications, comparison, and future perspectives. It has been observed that superficially porous columns may be the choice of future for ultrafast separations.
Introduction: Advances in HPLC Systems Technology
April 1st 2009During the last four to five years, chromatographers have witnessed some significant advances in technology, from the instrument perspective, with systems operating up to 15,000 psi using new and significantly improved detectors, sometimes operating in multiple dimensions, and from the column perspective, with smaller particle sizes and new chemistries and configurations.
Charged Aerosol Detection in Pharmaceutical Analysis: An Overview
April 1st 2009Over the past several years, charged aerosol detection (CAD) has become a widely used technology in the pharmaceutical laboratory. From formulation to stability and even quality control, many analysts are turning to this technology due to its advantages of sensitivity, ease of use, dynamic range, and applicability to a wide range of analyses in the drug development process. In this article, we will examine the operation and use of CAD in a regulated environment, briefly address method development and validation specifics, and highlight a few examples illustrating some of its advantages when used in the pharmaceutical laboratory.
Leading Healthcare Market Research Publisher Announces Growth for Liquid Chromatgraphy
April 1st 2009Kalorama Information, leading healthcare market research publisher, announced that Liquid chromatography will see significant growth from a new application - food safety testing - after several highly publicized food scandals.
Therapeutic Diazepam Monitoring in Human Plasma and Urine by HPLC: An Application for Alcoholism
April 1st 2009A rapid and simple high performance liquid chromatography (HPLC) method with basic extraction assays was developed to investigate free diazepam levels in the plasma and urine samples of patients medicated with this drug for the management of alcohol withdrawal syndrome. The HPLC analysis was optimized and evaluated for linearity, imprecision, recovery, detection and quantification limits. The method showed linearity between 50–500 ng/mL (r2 ≥ 0.990). Coefficients of variations (%CV) were calculated to be in the range of 1.77–9.60. According to ICH guidelines, theoretical limits of detection (LOD) and quantification (LOQ) for plasma and urine were calculated as 8.3 ng/mL, 27.5 ng/mL and 8.2 ng/mL, 26 ng/mL respectively. Diazepam monitoring in plasma and urine displayed remarkable variations. The importance of adjusting doses according to individual requirements and the routine monitoring of plasma or urine for patients under medication is highlighted.
Use of Orthogonal Methods During Pharmaceutical Development: Case Studies
April 1st 2009The primary goal of early phase development is to gain a fundamental knowledge of the chemistry of drug substances and drug products to facilitate optimization of synthetic schemes and drug product formulations. At the same time, methods are required for release and stability studies to support clinical trials. Ultimately, the knowledge gained during early development translates into designing control methods for commercial supplies. Our approach to meeting this challenge is based upon the use of a primary method along with orthogonal methods. This paper will discuss the overall strategy, with an emphasis on the chromatographic conditions selected to provide systematic othogonality for a broad range of drugs. Case studies will be presented to demonstrate the utility of orthogonal methods to resolve issues that could not have been addressed using a single release and stability method.
National Cancer Institute Purchases two Waters Corporation UPLC-MS Systems
April 1st 2009The National Cancer Institute's Center for Cancer Research (Bethesda, Maryland), purchased a Waters ACQUITY UPLC®/Synapt High Definition MS (HDMSTM) and Waters ACQUITY® UPLC/XevoTM TQ MS Systems for its metabolomics research programs.
Agilent Technologies Supports SFC System Development
April 1st 2009Agilent Technologies (Santa Clara, California) and Aurora SFC Systems (Sunnyvale, California) announced that Agilent is providing technical support for the development of Aurora?s SFC Fusion A5, a new instrument that converts existing high performance liquid chromatography (HPLC) systems into supercritical fluid chromatography (SFC) systems.