Liquid Chromatography (LC/HPLC)

An optimization strategy to obtain the best possible performance in the shortest analysis time for comprehensive off-line 2D-LC.

This month's column discusses the type of problems one can encounter when using methods supplied to us by other.

How to improve chromatographic performance using ultrapure water

This work demonstrates gains of up to 7-fold in signal to noise ratio with APCI mass spectrometry and reduction in analysis times of 5- to 8-fold

LEAP Technologies and PerkinElmer have entered into a resale agreement that will allow the company to incorporate PerkinElmer?s UHPLC and HPLC technology into the front-end automation systems it provides.

Discover the advantages of using UHPLC–MS–MS for lactotripeptide analysis

October 2006. The authors demonstrate the use of multiple-injection affinity capillary electrophoresis (MIACE) and several variations to MIACE to determine binding constants between the glycopeptide antibotics vancomycin, ristocetin, and teicoplanin from Streptomyces orientalis, Nocardia lurida, and Actinoplanes teichomyceticus, respectively, and D-Ala-D-Ala terminus peptides.

LabLogic Systems have been chosen by the Department of Medical Physics and Clinical Engineering at Addenbroke's Hospital in Cambridge, UK to supply the equipment to support the department?s metabolite analysis system for early-phase PET studies.

Remember the Rule of One - change just one thing at a time

This article facilitates the selection of various perfluorocarboxylic acids as ion-pair reagents in LC.

Eksigent Technologies has announced that it will be stepping up its manufacturing capabilities by 40%, as well as expanding the company?s servicing capabilities, to meet demand for its HPLC products.

Chromatographers with an interest in radio-HPLC flow-through detection are invited to book their place at LabLogic Systems' demonstration tour for the Model 5 Beta-RAM, which the company says has toured the UK, Switzerland and Holland and will also appear in France, Germany, Denmark and Sweden.

Phytronix Technologies and Agilent Technologies have signed an agreement to promote the Phytronix laser diode thermal desorption (LDTD) ion source with Agilent mass spectrometers.

Flavanones are a type of flavonoids occurring widely in plants, either in the aglycone backbone form itself or as glycosides. Flavonones exist in unique forms depending on the type of fruit and can be used to indicate the type of fruit that is being analysed. The following application introduces examples of high-speed analysis of flavanones in citrus juice using the Shimadzu ultra fast LC system, prominence UFLC, and the photodiode array detector SPD-M20A, focusing on narirutin, naringin, neohesperidin and hesperitin.

This article describes the use of combined ion chromatography-mass spectrometry (IC–MS) and ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP–MS) to analyse potentially harmful compounds.

The FDA has no objection to Rebaudioside A having a generally recognized as safe (GRAS) status as a sweetener for food and drink.1 Stevia sweeteners may contain other steviol glycosides as well, mainly Stevioside, Rebaudioside C and Dulcoside A. Because the FDA GRAS confirmation is only for the use of Rebaudioside A at 95% purity or above in food and beverages, stevia extracts must be highly purified and characterized prior to use.

Zhe Yin, Kenneth J. Fountain, Erin E. Chambers and Diane M. Diehl, Waters Corporation, Milford, Massachusetts, USA.

Based on the QuEChERS AOAC Official Method 2007.01, 16 pesticides were used for evaluating the performance of the Agilent's AOAC buffered extraction and SampliQ QuEChERS dispersive SPE kits for general fruits and vegetables.

Tobacco specific nitrosamines (TSNA) are a group of carcinogens found only in tobacco products and are formed from nicotine and related alkaloids during the production and processing of tobacco and tobacco products.

Polynuclear aromatic hydrocarbons (PAHs) are carcinogenic condensed ring aromatic compounds widely found as trace pollutants in waters, wastes, air particulates, soil and foods. PAHs can be routinely monitored using HPLC with a combination of UV and fluorescence detection as prescribed in EPA methods 550.1, 610 and 8310.

Polyaromatic hydrocarbons (PAH) are particularly relevant in the analysis of environmental pollution because of their ubiquity, toxicity and persistence. Consequently, the PAHs have become the most intensively studied pollutants in environmental analysis.

Salt formation is a critical aspect in drug development and HPLC is an important tool for determination of pharmaceutical counterions.

A method of sample preparation followed by reversed-phase high performance liquid chromatography (RP HPLC) with diode array (DA) detection.

Doug Kitson looks at some of Poland's leading separation science groups.

This is the fifth and final installment in a series of columns about various aspects of calibration of LC methods.

India is an increasingly influential player in the global pharmaceutical market. A pilot study, conducted in two major cities in India, Delhi and Chennai, explored the proliferation of substandard and counterfeit drugs in the market and discussed how the Indian state and federal governments could improve drug regulation and regulatory enforcement to combat this.

Liquid Chromatography users are asked about their experience with UHPLC

Faculty of Health Sciences at the University of Copenhagen will open the doors of its new research centre, The Novo Nordisk Foundation Centre for Protein Research. The centre is the result of an historic donation from the Novo Nordisk Foundation, which in 2007 gave the university €80 million for its establishment.

PerkinElmer and LEAP Technologies have entered into a resale agreement for ultra high performance liquid chromatography (UHPLC) and high performance liquid chromatography (HPLC) technology.

Waters Corporation (Milford, Massachusetts) announced that the U.S. Drug Enforcement Administration has purchased a Waters ACQUITY UltraPerformance liquid chromatograph for use in training new forensic chemists at the agency's Special Testing and Research Laboratory in Dulles, Virginia.