Liquid Chromatography (LC/HPLC)

We focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model

A relatively small set of columns can be used on a routine basis in different modes to meet modern analytical needs.

We focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model

This is John Dolan's 300th "LC Troubleshooting" column. Here, he takes a look through the archives.

John Dolan reaches a milestone with his 300th installment of "LC Troubleshooting" and takes a look back through the years.

This article aims to demonstrate a novel accelerated method development approach to develop HPLC and/or UPLC methods

This month Incognito reflects on his own laboratory's strategies for column care and shares their working practices for increasing the longevity of each column and preventing degradation.

In recent conversations at Pittcon 2010, it became clear to the author that sometimes we get too focused on the details of the method without backing up and determining how the fit into the big picture.

Michael Swartz presents his first annual review of HPLC systems and accessories at Pittcon 2010.

Part one focusses on ways of reducing and replacing solvents commonly used as mobile phases

Pat Sandra and his team will discuss how green chromatography works in practice

The separation of polar and hydrophilic compounds can be a challenge. Hydrophilic interaction liquid chromatography has proven to be a solid alternative for such applications.

As I travelled the miles of poster boards and sat through the various lectures, it became apparent that many of us still don?t know how to choose or optimize buffers for liquid phase separations.

Incognito comes up with a collection of rules of thumb for the relationships and mathematics behind web-based translators and calculators used for deriving results.

Oxytocin acetate samples were analysed successfully for both the peptide and acetate with a single method using a mixed-mode weak-anion-exchange column.

In this month's "LC Troubleshooting," the authors look at another use of calibration standards: to monitor the quality of the data, with a goal toward helping scientists troubleshoot instrument problems.

Rapid Method Development for Industrial LC Separations Using Automated Screening of Stationary Phases and Solvents.

An overview of current chromatography-based food toxicant screening is presented.

This article features some of the most frequently used HPLC applications for the characterization of biopharmaceuticals.

Various extraction methods for the simultaneous determination of veterinary drugs in several matrices by UHPLC–MS–MS are described.

Interviews from Pittcon with experts in the fields of GC, HPLC, MS/Hyphenated and Spectroscopy

The operating principles of the Direct-Electron Ionization interface, which is gaining popularity in many LC–MS applications

Experimental data are presented to help understand HPLC column retention in a way that should make column selection and method development proceed faster when initial results with C18 columns are disappointing.

Pavel Nesterenko from ACROSS about his work on the potential of diamond-related substances as stationary phases

This article provides an introduction to electrochemical liquid chromatography mass spectrometry.

Accelerating pharmaceutical studies by using LC-MS ion trap technology to conduct comprehensive metabolism profiling assays

The need for better methods for vitamin D analysis in a wide variety of foods is a good showcase for the advantages that ultrahigh-pressure liquid chromatography (UHPLC) can provide. The availability of systems that can operate as high as 1200 bar and an extensive range of UHPLC column choices provide the flexibility needed to rapidly develop fast vitamin D separations that can be adapted to meet the challenges of testing a diversity of food types

This article will demonstrate the feasibility of analysing complex mixtures without any prior sample preparation by making use of the high resolving power of both UHPLC and mass spectrometry.

The latest innovations in separation science will be revealed at Pittcon 2010 from 28 February–5 March 2010 in Florida, USA

John Dolan examines some questions he recently received concerning the changes that took place in the 2009 edition of the United States Pharmacopoeia.