Liquid Chromatography (LC/HPLC)

A simple and rapid HPLC method was established to simultaneously determine the active ingredients of red clover.

John Dolan presents some practical pointers to get the most out of your UHPLC system.

This article highlights a number of recent developments in HPLC for polyphenol analysis.

The guest authors describe an optimization procedure to achieve the highest plate count in a given analysis time.

Accurate and sensitive all-ions quantification using ultra-high resolution LC-MS

Ultra high performance liquid chromatography (UHPLC) systems, also referred to as fast LCs, are designed to take full advantage of smaller particles (sub 2 ?m), which yield higher resolution and increased sensitivity.

Speaking to Konik-Tech about the development of a new system combining LC and GC technology.

Using SRM and H-SRM on an Integrated UHPLC-MS Platform for Sensitive, Accurate and Reproducible Quantification of Perfluorinated Compounds in Human Breast Milk.

Practical HPLC, HILIC and RP-LC methods of analysing common food and beverages containing polyphenols are presented.

John Dolan discusses an inquiry he received from a reader complaining about the difficulty he was having meeting system suitability for an LC method that had been transferred into his lab.

The principles and attributes of today's HPLC detectors are discussed, and the advantages and disadvantages of the various detectors are compared and contrasted.

Incognito provides a retrospective account on subjects visited throughout the course of the year and asks if any problems have been addressed and what new problems have arisen.

A study published in the Journal of Separation Science has looked at using the racemization of amino acids as a proxy for DNA preservation, giving archaeologists a rapid screening method for samples too badly damaged to extract.

A simple rapid HPLC method for simultaneously determiing formononetin, biochanin A, daidzen and genistein in Red Clover

Guest author Stefan Brand and John Dolan take a look at how to speed up gradient separations while avoiding common errors.

The authors compare five bonded phases with bare silica for the SFC separation of a 19-component mixture of polar analytes.

Ron Majors presents his yearly review of the HPLC Conference.

An overview of current chromatography-based food toxicant screening is presented.

The authors describe a study of screening procedures that demonstrated a relatively small set of columns can be used routinely.

A broad peak where it does not belong is a classic sign of late elution.

The analysis of clozapine and norclozapine in plasma using automated on-line sample preparation and LC–MS/MS

Deterioration of the separation can result from many causes. This month's column looks at some techniques to isolate the problem source.

UHPLC is fast becoming mainstream in many laboratories. With increased data, however, comes a directly proportional increase in data integration.

Weikang Bio-Technology Group, a Chinese manufacturer and marketer of traditional Chinese medicines, is launching a new project to research and develop liquorice flavonoids for use in therapeutics. For the study, the company reports that it intends to use supercritical carbon dioxide extraction technology to produce superior quality herbal extracts, column chromatography to isolate and purify compounds and HPLC to separate, identify and quantify compounds based on their idiosyncratic polarities.

Global health and safety concerns now play a key role in driving innovation of liquid chromatography systems and the uptake of advanced LC technology, such as UHPLC.

John Dolan looks at a case in which the peak doesn't seem to belong with the neighboring peaks in the chromatogram.

The basics of IC are reviewed and recent advances that may extend this technology to new applications are discussed.

SFC is now regarded as a modern normal phase LC technique prompting a re-examination of the role of bare silica as a useful stationary phase.

A broad peak where it doesn't belong is a classic sign of late elution.

Atmospheric pressure gas chromatography is a technique that allows rapid switching between UPLC-MS and GC-MS applications.