Is HILIC Permanently Changing the Scene for HPLC Separations?
The separation of polar and hydrophilic compounds can be a challenge. Hydrophilic interaction liquid chromatography has proven to be a solid alternative for such applications.
Hydrophilic interaction liquid chromatography (HILIC) is a powerful technique for the separation of polar and hydrophilic compounds. In HILIC one uses a column with a hydrophilic stationary phase and an eluent with water, buffer and a high ratio of water-miscible organic solvent. Today it is generally settled that the retention mechanism is mainly based on partitioning between a water enriched layer, formed on a polar stationary phase under semi-aqueous conditions and a less polar mobile phase. A typical HILIC application uses 50–95% acetonitrile in an aqueous buffer such as ammonium formate, ammonium acetate and/or their corresponding acids or bases, which all have good solubility in organic solvents. HILIC can be used with many detection techniques, but when combined with evaporative detection techniques, such as electrospray ionization mass spectrometry, evaporative light scattering or charged aerosol detection, it will also enable higher sensitivities. A typical chromatogram and conditions are presented.
LCGC’s Year in Review: Highlights in Liquid Chromatography
December 20th 2024This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Next Generation Peak Fitting for Separations
December 11th 2024Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.
Mobile Phase Buffers in Liquid Chromatography: A Review of Essential Ideas
December 11th 2024In this installment of "LC Troubleshooting," Dwight Stoll discusses several essential principles related to when and why buffers are important, as well as practical factors, such as commonly used buffering agents, that are recommended for use with different types of detectors.
The Chromatographic Society 2025 Martin and Jubilee Award Winners
December 6th 2024The Chromatographic Society (ChromSoc) has announced the winners of the Martin Medal and the Silver Jubilee Medal for 2025. Professor Bogusław Buszewski of Nicolaus Copernicus University in Torun, Poland, has been awarded the prestigious Martin Medal, and the 2025 Silver Jubilee Medal has been awarded to Elia Psillakis of the Technical University of Crete in Greece.
