Liquid Chromatography (LC/HPLC)

Serial coupling of different column types can provide several important benefits to the chromatographer, including fine-tuning selectivity to separate complex mixtures.

The current challenges and future perspectives of the purification of cannabinoids from cannabis extracts are presented in this review article.

Pressure-enhanced liquid chromatography (PE-LC) offers a new approach for improving selectivity for large molecule separations. Examples shown here include short oligonucleotides in ion-pairing reversed-phase (IP-RP) liquid chromatography and larger nucleic acids in ion-exchange (IEX) chromatography.

A systematic approach to method improvement can save time and resources by using methods that are likely to be better, and more robust, than those developed using a trial-and-error approach.

The 2nd "Advances in Separation Science: From Extraction to Chromatographic Applications" workshop will be held from 28–29 June 2023 in the University of Liège in Belgium.

A novel LC–MS/MS method, with a dual ESI and APCI source using simple solvent extraction for sample preparation, was validated for analysis of 102 pesticides and 5 mycotoxins regulated by Colorado state in dried hemp.

Luis A. Colón is 2023's recipient of the Chromatography Forum of Delaware Valley Dal Nogare Award, which recognizes contributions to chromatographic science. The symposium took place at Pittcon 2023, which was held from March 18–22.

This article presents a new column technology that uses reversed phase separation and offers excellent peak shape, resolution, and robustness for a wide variety of food matrices.

When can analyte retention deviate from what is expected or normal? We explain three subtle causes.

On a fundamental level, equilibrium and diffusion define chemical separations. Practical applications of partition coefficients to chromatographic sample preparation illustrate this point.

A new automated active pharmaceutical ingredient (API) extraction approach with integrated analysis by online liquid chromatography (LC) to assess drug product potency is presented.

The new General Chapter <621> does reflect the great work of the Pharmacopoeias in helping chromatographers to use more modern technology for their regulated methods, speeding up analysis, and reducing waste. The latest revision allows us to adjust column dimensions for gradient separations, which is a significant advance. Although these freedoms are welcomed, one must be very mindful of the requirements or limits of the allowable changes and the verification work that underpins our demonstration of equivalent performance of the adjusted method.

Even relatively simple mixtures are not always easy to separate. What are the options for adjusting selectivity in reversed-phase LC separations?

The 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2023) will be held at the Conference Center Düsseldorf (CCD) in Germany, from 18–22 June 2023. Read on to find out what the organizers have in store for attendees.

Peter Schoenmakers and Emanuela Gionfriddo are the winners of the 16th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively.

This article discusses the main influences in oligonucleotide analysis using ion-pair (IP) reversed-phase chromatography.

A column with chemically modified column hardware showed improvements in analytical performance for siRNA compared to a conventional stainless-steel column.

In this first TRLC instalment, our recent developments, new possibilities, and current limitations of the use of 1D TRLC are discussed, while the column performance is described with respect to the fundamentals of HPLC.

In this latest instalment of our series on recurring challenges in liquid chromatography (LC), we turn our attention to peak widths that are wider than expected. Estimating the expected peak widths can help determine when a column and LC system are not working quite right, and to decide when troubleshooting should be initiated.

This month we interview Martina Catani from the University of Ferrara, Italy, about her work on the separation of enantiomers by high performance liquid chromatography/ultrahigh-pressure liquid chromatography (HPLC/UHPLC) and the study of the kinetic and thermodynamic properties of chiral stationary phases, and the separation and purification using LC analysis (single-column and continuous multi-column) of biomolecules of high therapeutic interest.

Webinar Date/Time: Thursday, February 16th, 2023 at 8am PST | 11am EST | 4pm GMT | 5 pm CET

When considering different ways to improve an established LC method, it helps to start by reviewing some essential concepts.

Robots outperform humans in five areas, and thus can be particularly beneficial for performing sample preparation for HPLC.

A harmonization of all conventional hold-up volume methods was successfully applied to RPLC, HILIC, AEX, and RP-AEX mixed-mode chromatography, regardless of the mobile phase.

Developing a short list of the likely causes of these results can help streamline our troubleshooting experience when sensitivity-related problems occur.

Webinar Date/Time: Thu, Jan 19, 2023 11:00 AM EST

Quantifying the content of coumarin and cinnamaldehyde in cinnamon products

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.

Estimating expected peak widths helps us determine when a column and LC system is not performing optimally.