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Advanced Antibody–Drug Conjugate Structural Characterization by Sheathless Capillary Electrophoresis–Tandem Mass Spectrometry Using Complementary ApproachesWith this method, a single injection was sufficient to characterize the amino acid sequence with complete sequence coverage. In addition, glycosylation and drug-loaded peptides could be identified from MS/MS spectra. A drug-loaded peptide fragmentation mass spectra study yielded drug-specific fragments, which reinforced the confidence about the identifications. The results reveal the ability of the sheathless CZE–MS/MS method to characterize an ADC’s primary structure in a single experiment.
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Advanced Antibody–Drug Conjugate Structural Characterization by Sheathless Capillary Electrophoresis–Tandem Mass Spectrometry Using Complementary ApproachesWith this method, a single injection was sufficient to characterize the amino acid sequence with complete sequence coverage. In addition, glycosylation and drug-loaded peptides could be identified from MS/MS spectra. A drug-loaded peptide fragmentation mass spectra study yielded drug-specific fragments, which reinforced the confidence about the identifications. The results reveal the ability of the sheathless CZE–MS/MS method to characterize an ADC’s primary structure in a single experiment.
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Direct Enantiomer-Selective Mass Spectrometry of Chiral Mixtures by Mass-Selected Photoelectron Circular DichroismSimultaneous, enantiomer-specific identification of chiral molecules in multicomponent mixtures is extremely challenging. With mass-selected photoelectron circular dichroism (MS-PECD) using an electron–ion coincidence imaging spectrometer, a compound can be identified as chiral without the need for any prior enantiomeric separation or enantiomer-selective complexation.
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Direct Enantiomer-Selective Mass Spectrometry of Chiral Mixtures by Mass-Selected Photoelectron Circular DichroismSimultaneous, enantiomer-specific identification of chiral molecules in multicomponent mixtures is extremely challenging. With mass-selected photoelectron circular dichroism (MS-PECD) using an electron–ion coincidence imaging spectrometer, a compound can be identified as chiral without the need for any prior enantiomeric separation or enantiomer-selective complexation.
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Appropriate Use of Mass Spectrometry in Clinical and Metabolic ResearchWhen adhering to sound analytical principles, the inclusion of mass spectrometry in the clinical laboratory can lead to accurate, selective, and precise quantitative methods by detecting new classes of compounds with greater efficiency and sensitivity than is possible with older, established technologies.
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Appropriate Use of Mass Spectrometry in Clinical and Metabolic ResearchWhen adhering to sound analytical principles, the inclusion of mass spectrometry in the clinical laboratory can lead to accurate, selective, and precise quantitative methods by detecting new classes of compounds with greater efficiency and sensitivity than is possible with older, established technologies.
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Appropriate Use of Mass Spectrometry in Clinical and Metabolic ResearchWhen adhering to sound analytical principles, the inclusion of mass spectrometry in the clinical laboratory can lead to accurate, selective, and precise quantitative methods by detecting new classes of compounds with greater efficiency and sensitivity than is possible with older, established technologies.
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Appropriate Use of Mass Spectrometry in Clinical and Metabolic ResearchWhen adhering to sound analytical principles, the inclusion of mass spectrometry in the clinical laboratory can lead to accurate, selective, and precise quantitative methods by detecting new classes of compounds with greater efficiency and sensitivity than is possible with older, established technologies.
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Breakthroughs in Biomarker ResearchJanet Kelsey spoke with Professor Bruno Domon, director of the new Luxembourg Clinical Proteomics Centre about the main purpose of his research group.
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High-Throughput Liquid–Liquid Extraction in 96-Well Format: Parallel Artificial Liquid Membrane ExtractionPALME almost eliminates consumption of hazardous solvents.
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High-Throughput Liquid–Liquid Extraction in 96-Well Format: Parallel Artificial Liquid Membrane ExtractionPALME almost eliminates consumption of hazardous solvents.
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High-Throughput Liquid–Liquid Extraction in 96-Well Format: Parallel Artificial Liquid Membrane ExtractionPALME almost eliminates consumption of hazardous solvents.
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Gas Chromatography–Vacuum Ultraviolet Absorbance Spectroscopy for the Quantitative Determination of Trace and Bulk Water in Organic SolventsRecent advances in vacuum ultraviolet (VUV) spectroscopy have allowed for the application of this technology as a chemical detection platform for gas chromatography (GC). This technique is known as GC–VUV. A GC–VUV detector can produce highly characteristic absorbance spectra for nearly all chemical species in the wavelength region of 125–240 nm. This enables not only identification but also robust quantitation of a variety of compounds separable by gas chromatography, including water. This article describes the results of a pilot study focused on trace water determination in common organic solvents using an ionic liquid stationary phase GC column in a GC–VUV platform.
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Automating Mycotoxin Analysis in BeveragesThe analysis of hazardous mycotoxins is crucially important in food to ensure the health of humans and animals. Here, a simple and fast analysis including the sample preparation and purification of mycotoxins within food is presented. Ten different mycotoxins were investigated simultaneously in 14 min. The method was applied to the analysis of mycotoxins in apple juice, grape juice, and two different batches of the same branded beer.
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High-Resolution Time-of-Flight Mass Spectrometry for Rapid Petroleum CharacterizationThe application of high-resolution TOF-MS to petroleomics is presented, and the basic theory of each type of ultrahigh-resolution mass spectral platform is briefly explained.
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Sophisticated Measures to Ensure Food Safety and IntegritySome of the threats to food safety and the measures in place for protection are discussed
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In-Depth Analysis of Host Cell Protein (HCP) Impurities by LC–MS/MS to Augment Routine HCP-ELISA Testing of BiotherapeuticsLC–MS/MS has gained momentum as an orthogonal approach to ELISA for host-cell protein (HCP) analysis. LC–MS/MS can identify and quantify individual HCPs, and help ensure that no HCPs evade detection above a reportable limit.
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Minimizing Method-Induced Deamidation and Isomerization During Antibody Characterization to Ensure Optimal Understanding of Product Quality AttributesA new peptide mapping method was developed specifically for mAb characterization that employs optimal enzyme pH for robustness, but with short digestion times and time-course elements to minimize and monitor deamidation–isomerization, respectively, enabling a more accurate assessment of potential CDR sequence liabilities.
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Minimizing Method-Induced Deamidation and Isomerization During Antibody Characterization to Ensure Optimal Understanding of Product Quality AttributesA new peptide mapping method was developed specifically for mAb characterization that employs optimal enzyme pH for robustness, but with short digestion times and time-course elements to minimize and monitor deamidation–isomerization, respectively, enabling a more accurate assessment of potential CDR sequence liabilities.
Latest:
Minimizing Method-Induced Deamidation and Isomerization During Antibody Characterization to Ensure Optimal Understanding of Product Quality AttributesA new peptide mapping method was developed specifically for mAb characterization that employs optimal enzyme pH for robustness, but with short digestion times and time-course elements to minimize and monitor deamidation–isomerization, respectively, enabling a more accurate assessment of potential CDR sequence liabilities.
Latest:
Minimizing Method-Induced Deamidation and Isomerization During Antibody Characterization to Ensure Optimal Understanding of Product Quality AttributesA new peptide mapping method was developed specifically for mAb characterization that employs optimal enzyme pH for robustness, but with short digestion times and time-course elements to minimize and monitor deamidation–isomerization, respectively, enabling a more accurate assessment of potential CDR sequence liabilities.
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Establishing USP Rebaudioside A and Stevioside Reference Standards for the Food Chemicals CodexHere's how these new reference standards were characterized.
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The Use of Extraction Technologies in Food Safety StudiesTraditional extraction methods for food samples, such as liquid–liquid extraction and Soxhlet extraction, are often time-consuming and require large amounts of organic solvents. Therefore, one of the objectives of analytical food safety studies currently has been the development of new extraction techniques that can improve the accuracy and precision of analytical results and simplify the analytical procedure.
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Green Chromatography (Part 3): Sample Preparation TechniquesDiscussing the latest developments in sample preparation techniques that reduce environmental impact.
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Testing the Critical Interface: Leachables and ExtractablesCoping with extractables and leachables testing is easier when you have a good understanding of the issues involved. Information from public databases and industry consortia also is invaluable.
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What’s New in Reversed-Phase Liquid Chromatography Selectivity? An Update from the Perspective of the Hydrophobic Subtraction ModelIt has been more than five years since the last update in this column on the evolution of the Hydrophobic Subtraction Model (HSM) of reversed-phase selectivity and characteristics of new stationary phases recently characterized using the model. In this installment, Dwight Stoll discusses the continuing evolution of the model.
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A New Twist to Ion-Pairing Chromatography: In-Sample Addition of Ion-Pairing ReagentIon-pairing chromatography has traditionally been implemented with the addition of an ion-pairing reagent into the mobile phase. Here, an alternative method is presented, in which the ion-pairing reagent is deposited on column as a sample additive instead of in the mobile phase.
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Analysis of Polymers and Protein Nanoparticles using Asymmetrical Flow Field-Flow Fractionation (AF4)This article describes some of the latest developments for the analysis of polymers and nanoparticles.
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UHPLC or HPLC? The Choice is YoursSergio Guazzotti speaks to Janet Kelsey about the advantages of UHPLC over HPLC and issues to consider when selecting this technique.
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GC/GC–MSA snapshot of key trends and developments in the GC/GC–MS sector according to selected panelists from companies exhibiting at Analytica 2018.
