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This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.

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Fast protein liquid chromatography (FPLC) and high performance liquid chromatography (HPLC): two liquid chromatographic methods in direct comparison. This article discusses the differences between the two techniques with particular emphasis on the requirements of their respective analytes.

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Systematic toxicological analysis is an important step in medicolegal investigations of death, poisoning, and drug use. The primary goal is the detection and confirmation of potentially toxic compounds in evidence. This article describes a workflow using nontargeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) for reliable compound identification.

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Systematic toxicological analysis is an important step in medicolegal investigations of death, poisoning, and drug use. The primary goal is the detection and confirmation of potentially toxic compounds in evidence. This article describes a workflow using nontargeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) for reliable compound identification.

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Victor U. Weiss and Günter Allmaier from TU Vien discuss how combining molecular beacons with chip capillary electrophoresis (CE) offers scope to investigate viral RNA replication and improve gene therapy delivery systems.

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This article describes the use of comprehensive two-dimensional gas chromatography (GC×GC) with time-of-flight mass spectrometry (TOF-MS) and tandem ionization for characterization and comparison of the composition of essential oils, aiding rigorous quality control.

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PCBs are a group of synthetic organic chemicals that contain 209 individual compounds (known as congeners) with varying harmful effects. PCBs enter the environment as mixtures containing a variety of individual components.

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The analysis of pesticide residues in food samples from the state of Connecticut's regulatory monitoring program are compared to USDA and US FDA results.

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Ion-pairing chromatography has traditionally been implemented with the addition of an ion-pairing reagent into the mobile phase. Here, an alternative method is presented, in which the ion-pairing reagent is deposited on column as a sample additive instead of in the mobile phase.

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Pickering Laboratories, Inc. offers a complete solution for Amino Acids Analysis according to European Pharmacopoeia 8.0. This includes the Pinnacle PCX post-column derivatization instru¬ment, analytical columns and GARDs, buffers and Trione Ninhydrin reagent. The methods presented in this application note were optimized to comply with system suitability requirements of Pharmacopoeia 8.0 methods.

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A few simple calculations and estimates can provide insight into a troublesome chromatogram.

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As Klaus Unger turns 80, we review his contributions to the field.

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As Klaus Unger turns 80, we review his contributions to the field.

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The analysis of oil samples containing many thousands of constituents best illustrates the benefits of ion mobility MS for complex samples. Here, we test the limits of ion mobility MS to discern differences between batches of Copaxone, a highly complex drug containing billions of peptides, and various purported generic versions of the drug.

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An automated method for determination of water in liquid sweeteners was developed using headspace gas chromatography (GC) and ionic liquid-based capillary GC columns. This method allowed for the rapid determination of water with minimal sample pretreatment. In addition to providing fast analysis time for the samples, the headspace GC method was found to be accurate and precise for the measurement of water in 16 liquid sweeteners. This method was shown to be widely applicable for sugar and sugar-free sweeteners and more accurate than Karl Fischer titration.

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A method based on salting-out assisted liquid–liquid extraction for the analysis of α-dicarbonyls in wines was developed. The sample preparation procedure consists of a single step, involving the simultaneous extraction and derivatization of the analytes using an o-phenylenediamine–acetonitrile solution with sodium chloride as the salting-out agent. The obtained organic phase is collected and directly analyzed by liquid chromatography with spectrophotometric detection. The studied α-dicarbonyls were determined in eight wines. The developed methodology substantially reduces the complexity of the sample matrix, which is a very important aspect in routine analysis, especially to ensure long-lasting and reliable functioning of the chromatographic systems, while being a new and attractive methodology for the analysis of α-dicarbonyls.

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A method based on salting-out assisted liquid–liquid extraction for the analysis of α-dicarbonyls in wines was developed. The sample preparation procedure consists of a single step, involving the simultaneous extraction and derivatization of the analytes using an o-phenylenediamine–acetonitrile solution with sodium chloride as the salting-out agent. The obtained organic phase is collected and directly analyzed by liquid chromatography with spectrophotometric detection. The studied α-dicarbonyls were determined in eight wines. The developed methodology substantially reduces the complexity of the sample matrix, which is a very important aspect in routine analysis, especially to ensure long-lasting and reliable functioning of the chromatographic systems, while being a new and attractive methodology for the analysis of α-dicarbonyls.

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Using a liquid chromatography–mass spectrometry (LC–MS) method in conjunction with two complementary types of chromatographic retention modes-reversed phase and aqueous normal phase-various compounds present in mesquite flour extracts were identified. Because of the diverse types of chemical constituents found in such natural product extracts, a single chromatographic mode may not be sufficient for a comprehensive characterization. However, the combination of reversed-phase and aqueous normal phase LC can encompass a wide range of analyte polarity. This characterization of the composition of mesquite flour could be used in future studies to elucidate the beneficial health effects of its consumption.

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Using a liquid chromatography–mass spectrometry (LC–MS) method in conjunction with two complementary types of chromatographic retention modes-reversed phase and aqueous normal phase-various compounds present in mesquite flour extracts were identified. Because of the diverse types of chemical constituents found in such natural product extracts, a single chromatographic mode may not be sufficient for a comprehensive characterization. However, the combination of reversed-phase and aqueous normal phase LC can encompass a wide range of analyte polarity. This characterization of the composition of mesquite flour could be used in future studies to elucidate the beneficial health effects of its consumption.

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Using a liquid chromatography–mass spectrometry (LC–MS) method in conjunction with two complementary types of chromatographic retention modes-reversed phase and aqueous normal phase-various compounds present in mesquite flour extracts were identified. Because of the diverse types of chemical constituents found in such natural product extracts, a single chromatographic mode may not be sufficient for a comprehensive characterization. However, the combination of reversed-phase and aqueous normal phase LC can encompass a wide range of analyte polarity. This characterization of the composition of mesquite flour could be used in future studies to elucidate the beneficial health effects of its consumption.

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Using a liquid chromatography–mass spectrometry (LC–MS) method in conjunction with two complementary types of chromatographic retention modes-reversed phase and aqueous normal phase-various compounds present in mesquite flour extracts were identified. Because of the diverse types of chemical constituents found in such natural product extracts, a single chromatographic mode may not be sufficient for a comprehensive characterization. However, the combination of reversed-phase and aqueous normal phase LC can encompass a wide range of analyte polarity. This characterization of the composition of mesquite flour could be used in future studies to elucidate the beneficial health effects of its consumption.

Latest:

Using a liquid chromatography–mass spectrometry (LC–MS) method in conjunction with two complementary types of chromatographic retention modes-reversed phase and aqueous normal phase-various compounds present in mesquite flour extracts were identified. Because of the diverse types of chemical constituents found in such natural product extracts, a single chromatographic mode may not be sufficient for a comprehensive characterization. However, the combination of reversed-phase and aqueous normal phase LC can encompass a wide range of analyte polarity. This characterization of the composition of mesquite flour could be used in future studies to elucidate the beneficial health effects of its consumption.

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The authors developed a new analytical HPLC method using a silica hydride-based column to analyze mushrooms.

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Describing developments in LC–MS and GC–MS that reveal the ageing chemistry of whiskey aged in oak barrels.

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The determination of the chemical composition distribution of acrylic resins is not straightforward. Pyrolysis–gas chromatography (py-GC) analyses of acrylic resins can yield problems in the recovery of the hydroxyl and acid functional fragments that form. To overcome these problems pyrolysis–liquid chromatography (py-LC) can be performed. This article describes how the validation and quantification of hydroxyl acrylate-, hydroxyl methacrylate-, hydroxyl propylacrylate-, and hydroxyl propylmethacrylate resins by py-LC is performed. Furthermore, off-line size-exclusion chromatography (SEC) coupled to py-LC is performed to determine the chemical composition distribution over the molecular weight distribution of a core–shell waterborne acrylic resin.