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Separation of Amino Acid Enantiomers Using Capillary Electrophoresis with a New Chiral LigandA chiral ionic liquid, namely 1-ethyl-3-methyl imidazole L-tartrate ([EMIM][L-Tar]), was applied as a new chiral ligand for the separation of tryptophan, tyrosine, and phenylalanine enantiomers by chiral ligand exchange capillary electrophoresis (CE). To validate the unique behaviour of [EMIM][L-Tar], the performance of L-tartaric acid and 1-ethyl-3-methyl imidazole L-proline as chiral ligands was investigated to make a comparison with [EMIM][L-Tar]. Then the separation mechanism was further discussed. It was proven that [EMIM][L-Tar] was a good chiral ligand and would have good application prospects in separation science.
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Biolayer Interferometry as an Alternative to HPLC for Measuring Product Concentration in Fermentation BrothSeveral key applications of biolayer interferometry in pharmaceutical development have emerged recently. Here, we evaluate its use for easuring product titer from fermentation, and compare the strengths and weaknesses of the technique to those of HPLC.
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Flavour and Fragrance Analysis: Wondrous VanillaThere are over 100 different types of vanilla, all characterized by different aroma profiles. To determine vanilla origin, and for quality control purposes, laboratories typically rely on headspace or thermal desorption techniques used in combination with gas chromatography–mass spectrometry (GC–MS). This article explains more.
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GC Troubleshooting in Petrochemical AnalysisA guide to simple troubleshooting steps in gas chromatography (GC) with an emphasis on petrochemical analysis.
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Gingerol purification with Centrifugal Partition Chromatography SCPC-250Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.
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Gingerol purification with Centrifugal Partition Chromatography SCPC-250Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.
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A Simple and Effective ICP-MS Method Capable of Detecting Trace and Major Elements in Milk SamplesThe method described here is a simple method for detection of a wide spectrum of metals and for quantitative analysis over a wide dynamic range of elements, including both toxic metals and those metals essential for health and well-being.
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Fast Screening and Quantification of Pesticides Using an LC-MS Solution – The Pesticides Explorer CollectionA fully tested LC-MS/MS workflow for rapid and robust quantification of more than 250 pesticides below maximum residue limits (MRLs) with sensitivity, accuracy, and precision that meets stringent EU guidelines.
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Fast Screening and Quantification of Pesticides Using an LC-MS Solution – The Pesticides Explorer CollectionA fully tested LC-MS/MS workflow for rapid and robust quantification of more than 250 pesticides below maximum residue limits (MRLs) with sensitivity, accuracy, and precision that meets stringent EU guidelines.
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Fast Screening and Quantification of Pesticides Using an LC-MS Solution – The Pesticides Explorer CollectionA fully tested LC-MS/MS workflow for rapid and robust quantification of more than 250 pesticides below maximum residue limits (MRLs) with sensitivity, accuracy, and precision that meets stringent EU guidelines.
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Analyzing Antarctic Ice Cores Using Capillary ICIce cores contain an abundance of information about climate and the changes it is undergoing. Brett Paull and Estrella Sanz Rodriguez from the Australian Centre for Research on Separation Science (ACROSS) at the University of Tasmania, Hobart, Australia, spoke to Kate Mosford of The Column about their work on the analysis of Antarctic ice cores and the important role of capillary ion chromatography (cap-IC) in this area of research.
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Portable and Field‑Deployable Liquid Chromatography for Environmental StudiesLiquid chromatography (LC) is a platform technology amenable to portable and “at-site” or deployable applications. This has awoken end-users to new possibilities and potential cost savings and process improvements.
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Matrix-Assisted Laser Desorption-Ionization Imaging Mass Spectrometry for Direct Tissue AnalysisA summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS
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Improved Analysis of Polyfluorinated Alkyl Substances in Environmental Samples Using Optimized ASTM Method 7968/7979This article evaluates ASTM D7979-16 for the “direct” analysis of 30 PFCs and 19 mass-labeled surrogates. Instead of using solid-phase extraction, the PFCs were solubilized in a methanol–water mix and filtered if necessary, and the sample was injected into a LC–MS/MS system.
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Improved Analysis of FAMEs using Gas Chromatography with Vacuum Ultraviolet Detection (GC–VUV)The powerful characteristics of gas chromatography (GC) with VUV (vacuum ultraviolet) detection for the analysis of saturated and unsaturated fatty acids are described.
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The Direct Analysis of Diquat and Paraquat in Lake Water Samples by per Aqueous Liquid ChromatographyThe authors discuss an LC method for the direct analysis of diquat and paraquat in lake water.
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A Multiphase Sampling and Analytical Approach for Investigating Airborne PFAS TransmissionThe investigation of airborne PFAS transmission is important in preserving the outdoor urban environment. The authors explain why GC–MS/MS and LC–MS/MS are the best techniques for analyzing airborne PFAS.
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New Method US EPA 625 with Solid Phase Extraction for Challenging WastewatersThis automated SPE methodology is a solution that will reduce analyst labor, solvent usage, and turn-around-time while maintaining the high quality results required within today's laboratories.
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New Method for Sampling and Analysis of Electronic Cigarette AerosolsFollowing sampling, tubes were transferred to a Markes UNITY™ thermal desorption system paired with an Agilent 7890B GC that was coupled to an Agilent 5977A MS detector. The UNITY™ system and GC–MS parameters are presented in Table I and Table II, respectively.
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New Method for Sampling and Analysis of Electronic Cigarette AerosolsFollowing sampling, tubes were transferred to a Markes UNITY™ thermal desorption system paired with an Agilent 7890B GC that was coupled to an Agilent 5977A MS detector. The UNITY™ system and GC–MS parameters are presented in Table I and Table II, respectively.
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Increased Resolution and Reproducibility with TSKgel® UP-SW3000 Columns Versus a Competitive ColumnTSKgel UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products. Higher resolution can be achieved for the separation of antibody monomers, dimers, and higher order aggregates with a TSKgel UP-SW3000 column compared to a competitor UHPLC column. The TSKgel UP-SW3000 column provided excellent reproducibility for the peak parameters of retention time, asymmetry, and column efficiency. As demonstrated by the %RSD values, injection-to-injection reproducibility was superior to the competitor column.
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Optimization of the Electrospray Ionization Source with the Use of the Design of Experiments Approach for the LC–MS-MS Determination of Selected Metabolites in Human UrineThis article presents a step-by-step DoE optimization strategy for an electrospray ionization source with the aim to quantitatively analyze 18 compounds in a metabolomic study of human urine.
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Optimization of the Electrospray Ionization Source with the Use of the Design of Experiments Approach for the LC–MS-MS Determination of Selected Metabolites in Human UrineThis article presents a step-by-step DoE optimization strategy for an electrospray ionization source with the aim to quantitatively analyze 18 compounds in a metabolomic study of human urine.
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Optimization of the Electrospray Ionization Source with the Use of the Design of Experiments Approach for the LC–MS-MS Determination of Selected Metabolites in Human UrineThis article presents a step-by-step DoE optimization strategy for an electrospray ionization source with the aim to quantitatively analyze 18 compounds in a metabolomic study of human urine.
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Optimization of the Electrospray Ionization Source with the Use of the Design of Experiments Approach for the LC–MS-MS Determination of Selected Metabolites in Human UrineThis article presents a step-by-step DoE optimization strategy for an electrospray ionization source with the aim to quantitatively analyze 18 compounds in a metabolomic study of human urine.
Latest:
Optimization of the Electrospray Ionization Source with the Use of the Design of Experiments Approach for the LC–MS-MS Determination of Selected Metabolites in Human UrineThis article presents a step-by-step DoE optimization strategy for an electrospray ionization source with the aim to quantitatively analyze 18 compounds in a metabolomic study of human urine.
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Liquid Chromatography Tandem Mass Spectrometry Method for Novel Psychoactive Substances: Kratom and Synthetic Cathinones in UrineA novel “dilute-and-shoot” LC–MS/MS method is described for the analysis of “bath salts” sold as “legal” highs, including mitragynine and nine synthetic cathinones, in urine.
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Light and Medium Petroleum Distillate Characterization Using Two-Dimensional Gas Chromatography–High-Resolution Time-of-Flight Mass Spectrometry and Enhanced Software ProcessingEnhanced chromatographic resolution (GC×GC), increased resolving power with high-resolution TOF-MS, and software designed to leverage these attributes are combined to provide data that are easy to process and interpret.
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Extending the Hydrocarbon Range for the Analysis of Soil Gas Samples Using Automated Thermal Desorption Coupled with Gas Chromatography–Mass SpectrometryThis study describes the recovery of compounds above the boiling point of naphthalene achieved by optimizing the thermal desorption chemistry for the determination of volatile organic compounds ranging from C3 to C26 in soil gas samples using Method TO-17. Figures of merit such as breakthrough, precision, linearity, and detection capability are presented, in addition to an evaluation of its real-world capability at sites with moderate diesel and semivolatile polynuclear aromatic hydrocarbon (up to pyrene) contamination, in the presence of high humidity. This research has provided a means to determine a more representative composition of soil gas.
