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Gingerol purification with Centrifugal Partition Chromatography SCPC-250Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.
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A New Path to High-Resolution HPLC–TOF-MS — Survey, Targeted, and Trace Analysis Applications of TOF-MS in the Analysis of Complex Biochemical MatricesHigh performance time-of-flight mass spectrometry (TOF-MS) is applied to the analysis and characterization of complex biological samples. High mass accuracy, high resolution, and accurate relative isotope abundance are all applied to the determination of analytes covering a range of concentrations in complex matrices including plasma and urine. Qualitative and quantitative evaluations are provided and include demonstrations of the impact of high-performance MS on sensitivity and selectivity. The ability to leverage high-performance MS in conjunction with a broad dynamic range in rapid ultrahigh-pressure liquid chromatography (UHPLC) analyses to identify unknowns and to propose putative metabolic biomarkers is demonstrated. The impact of speed of analysis and selectivity to the depth of coverage and accuracy of the analyses are discussed.
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Ultrapure Water for HPLC and UHPLCIt is important for the standards, organic solvents, and HPLC mobile phases to be of the highest purity, but water quality is often taken for granted.
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A Study on the Optimization of the Ion-Pair Reversed Phase Liquid Chromatography Analysis of Protected And Unprotected DNA OligonucleotidesThis article describes a comprehensive study on optimizing ion-pair reversed-phase liquid chromatography for analysing protected and unprotected single-stranded DNA oligonucleotides.
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Self-Assembled Nanomaterials for Enhanced Chemical SeparationsNanomaterials offer exciting possibilities for improving separations with techniques such as TLC, SPME, and CE.
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Authentication and Routine Screening of Ginsenoside Isomers in Functional Food Products: UHPLC Coupled with Ion Mobility Mass SpectrometryGinseng has been used worldwide for thousands of years. Thought to possess therapeutic effects, it has been marketed as a natural product for the treatment of disease. This article describes how ultrahigh-performance liquid chromatography (UHPLC) can be coupled with ion mobility mass spectrometry (IMS-MS) to profile phytochemicals contained within ginseng and confirm quality.
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Authentication and Routine Screening of Ginsenoside Isomers in Functional Food Products: UHPLC Coupled with Ion Mobility Mass SpectrometryGinseng has been used worldwide for thousands of years. Thought to possess therapeutic effects, it has been marketed as a natural product for the treatment of disease. This article describes how ultrahigh-performance liquid chromatography (UHPLC) can be coupled with ion mobility mass spectrometry (IMS-MS) to profile phytochemicals contained within ginseng and confirm quality.
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Establishing USP Rebaudioside A and Stevioside Reference Standards for the Food Chemicals CodexHere's how these new reference standards were characterized.
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Nontargeted Metabolite Profiling in Next-Generation Plant Breeding: A Case Study in Malting BarleyThe power of nontargeted metabolite profiling is illustrated in a study focused on the determination of molecular markers in malting barley that are predictive of desirable malting quality for brewing applications. The metabolite extraction, detection, and analysis methods are high throughput and reproducible, and therefore, this approach represents a practical addition to the plant breeder’s molecular toolbox.
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Screening for Hundreds of Pesticide Residues Using a GC/Q-TOF with an Exact Mass Pesticide Database in FoodThis note discusses how to rapidly screen, identify, and generate quantitative information for pesticide residues in food with the Agilent 7200 GC/Q-TOF with Agilent MassHunter Qualitative Analysis All Ions workflow.
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Tools to Improve Protein SeparationsThe capability to separate and analyze a wide range of proteins in complex systems remains a prime requirement in the biochemical sciences. Intact protein separations are especially difficult as these large molecules can present different conformations, association states and amphoteric features with chromatographic surfaces. Combining high performance liquid chromatography (HPLC) and ultrahigh pressure liquid chromatography (UHPLC) with mass spectrometry (MS) has proven to be an effective approach for solving difficult problems involving protein analyses. Considerable effort has been made to develop columns for separating proteins with high efficiency for reversed-phase, ion-exchange, size-exclusion chromatography, hydrophilic interaction liquid chromatography (HILIC), and hydrophobic interaction chromatography (HIC). Even so, many situations still exist where insufficient resolution is available for accurate protein analysis even when high-resolution MS is available. This presentation provides a brief overview of new approaches being investigated in the author's laboratories for obtaining superior protein separations. This includes new approaches for obtaining better protein separations with columns of highly-efficient superficially porous silica particles and techniques using MS-friendly mobile phases with effective methods for changing protein selectivity (band spacings) by column type and organic mobile phase modifiers.
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Tools to Improve Protein SeparationsThe capability to separate and analyze a wide range of proteins in complex systems remains a prime requirement in the biochemical sciences. Intact protein separations are especially difficult as these large molecules can present different conformations, association states and amphoteric features with chromatographic surfaces. Combining high performance liquid chromatography (HPLC) and ultrahigh pressure liquid chromatography (UHPLC) with mass spectrometry (MS) has proven to be an effective approach for solving difficult problems involving protein analyses. Considerable effort has been made to develop columns for separating proteins with high efficiency for reversed-phase, ion-exchange, size-exclusion chromatography, hydrophilic interaction liquid chromatography (HILIC), and hydrophobic interaction chromatography (HIC). Even so, many situations still exist where insufficient resolution is available for accurate protein analysis even when high-resolution MS is available. This presentation provides a brief overview of new approaches being investigated in the author's laboratories for obtaining superior protein separations. This includes new approaches for obtaining better protein separations with columns of highly-efficient superficially porous silica particles and techniques using MS-friendly mobile phases with effective methods for changing protein selectivity (band spacings) by column type and organic mobile phase modifiers.
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Extraction Method for Pesticides in Poultry FatUsing a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.
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Extraction Method for Pesticides in Poultry FatUsing a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.
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Advances in Chromatography for the Optimal Separation of Pesticides in FoodWhat critical components can help to resolve the separation challenges related to pesticides analysis in food?
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Extraction Method for Pesticides in Poultry FatUsing a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.
Latest:
Extraction Method for Pesticides in Poultry FatUsing a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.
Latest:
Extraction Method for Pesticides in Poultry FatUsing a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.
Latest:
Extraction Method for Pesticides in Poultry FatUsing a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.
