This article provides useful tips for smooth validation of multi-analyte LC–MS-MS methods and summarizes important validation outcomes for 295 analytes, including more than 200 mycotoxins.
This article provides useful tips for smooth validation of multi-analyte LC–MS-MS methods and summarizes important validation outcomes for 295 analytes, including more than 200 mycotoxins.
This article provides useful tips for smooth validation of multi-analyte LC–MS-MS methods and summarizes important validation outcomes for 295 analytes, including more than 200 mycotoxins.
This article provides useful tips for smooth validation of multi-analyte LC–MS-MS methods and summarizes important validation outcomes for 295 analytes, including more than 200 mycotoxins.
Using a bonded polymeric zwitterionic stationary phase, in HILIC mode, polar quaternary herbicides like Diquat can easily be retained, with good peak symmetry. The impact of ionic strength and organic content in the mobile phase is discussed.
Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.
Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.
High performance time-of-flight mass spectrometry (TOF-MS) is applied to the analysis and characterization of complex biological samples. High mass accuracy, high resolution, and accurate relative isotope abundance are all applied to the determination of analytes covering a range of concentrations in complex matrices including plasma and urine. Qualitative and quantitative evaluations are provided and include demonstrations of the impact of high-performance MS on sensitivity and selectivity. The ability to leverage high-performance MS in conjunction with a broad dynamic range in rapid ultrahigh-pressure liquid chromatography (UHPLC) analyses to identify unknowns and to propose putative metabolic biomarkers is demonstrated. The impact of speed of analysis and selectivity to the depth of coverage and accuracy of the analyses are discussed.
It is important for the standards, organic solvents, and HPLC mobile phases to be of the highest purity, but water quality is often taken for granted.
This article describes a comprehensive study on optimizing ion-pair reversed-phase liquid chromatography for analysing protected and unprotected single-stranded DNA oligonucleotides.
Nanomaterials offer exciting possibilities for improving separations with techniques such as TLC, SPME, and CE.
Ginseng has been used worldwide for thousands of years. Thought to possess therapeutic effects, it has been marketed as a natural product for the treatment of disease. This article describes how ultrahigh-performance liquid chromatography (UHPLC) can be coupled with ion mobility mass spectrometry (IMS-MS) to profile phytochemicals contained within ginseng and confirm quality.
Ginseng has been used worldwide for thousands of years. Thought to possess therapeutic effects, it has been marketed as a natural product for the treatment of disease. This article describes how ultrahigh-performance liquid chromatography (UHPLC) can be coupled with ion mobility mass spectrometry (IMS-MS) to profile phytochemicals contained within ginseng and confirm quality.
Here's how these new reference standards were characterized.
The power of nontargeted metabolite profiling is illustrated in a study focused on the determination of molecular markers in malting barley that are predictive of desirable malting quality for brewing applications. The metabolite extraction, detection, and analysis methods are high throughput and reproducible, and therefore, this approach represents a practical addition to the plant breeder’s molecular toolbox.
This note discusses how to rapidly screen, identify, and generate quantitative information for pesticide residues in food with the Agilent 7200 GC/Q-TOF with Agilent MassHunter Qualitative Analysis All Ions workflow.
The capability to separate and analyze a wide range of proteins in complex systems remains a prime requirement in the biochemical sciences. Intact protein separations are especially difficult as these large molecules can present different conformations, association states and amphoteric features with chromatographic surfaces. Combining high performance liquid chromatography (HPLC) and ultrahigh pressure liquid chromatography (UHPLC) with mass spectrometry (MS) has proven to be an effective approach for solving difficult problems involving protein analyses. Considerable effort has been made to develop columns for separating proteins with high efficiency for reversed-phase, ion-exchange, size-exclusion chromatography, hydrophilic interaction liquid chromatography (HILIC), and hydrophobic interaction chromatography (HIC). Even so, many situations still exist where insufficient resolution is available for accurate protein analysis even when high-resolution MS is available. This presentation provides a brief overview of new approaches being investigated in the author's laboratories for obtaining superior protein separations. This includes new approaches for obtaining better protein separations with columns of highly-efficient superficially porous silica particles and techniques using MS-friendly mobile phases with effective methods for changing protein selectivity (band spacings) by column type and organic mobile phase modifiers.
The capability to separate and analyze a wide range of proteins in complex systems remains a prime requirement in the biochemical sciences. Intact protein separations are especially difficult as these large molecules can present different conformations, association states and amphoteric features with chromatographic surfaces. Combining high performance liquid chromatography (HPLC) and ultrahigh pressure liquid chromatography (UHPLC) with mass spectrometry (MS) has proven to be an effective approach for solving difficult problems involving protein analyses. Considerable effort has been made to develop columns for separating proteins with high efficiency for reversed-phase, ion-exchange, size-exclusion chromatography, hydrophilic interaction liquid chromatography (HILIC), and hydrophobic interaction chromatography (HIC). Even so, many situations still exist where insufficient resolution is available for accurate protein analysis even when high-resolution MS is available. This presentation provides a brief overview of new approaches being investigated in the author's laboratories for obtaining superior protein separations. This includes new approaches for obtaining better protein separations with columns of highly-efficient superficially porous silica particles and techniques using MS-friendly mobile phases with effective methods for changing protein selectivity (band spacings) by column type and organic mobile phase modifiers.