Author


Alexandra Malachova

Latest:

Multi-Toxin Determination in Food: The Power of “Dilute and Shoot” Approaches in LC–MS–MS

This article provides useful tips for smooth validation of multi-analyte LC–MS-MS methods and summarizes important validation outcomes for 295 analytes, including more than 200 mycotoxins.


Rudolf Krska

Latest:

Multi-Toxin Determination in Food: The Power of “Dilute and Shoot” Approaches in LC–MS–MS

This article provides useful tips for smooth validation of multi-analyte LC–MS-MS methods and summarizes important validation outcomes for 295 analytes, including more than 200 mycotoxins.


Franz Berthiller

Latest:

Multi-Toxin Determination in Food: The Power of “Dilute and Shoot” Approaches in LC–MS–MS

This article provides useful tips for smooth validation of multi-analyte LC–MS-MS methods and summarizes important validation outcomes for 295 analytes, including more than 200 mycotoxins.


Michael Sulyok

Latest:

Multi-Toxin Determination in Food: The Power of “Dilute and Shoot” Approaches in LC–MS–MS

This article provides useful tips for smooth validation of multi-analyte LC–MS-MS methods and summarizes important validation outcomes for 295 analytes, including more than 200 mycotoxins.


Patrik Appelblad

Latest:

Separation of Quaternary Ammonium Compounds Using a Bonded Polymeric Zwitterionic Stationary Phase

Using a bonded polymeric zwitterionic stationary phase, in HILIC mode, polar quaternary herbicides like Diquat can easily be retained, with good peak symmetry. The impact of ionic strength and organic content in the mobile phase is discussed.



Céline Le Quémener

Latest:

Gingerol purification with Centrifugal Partition Chromatography SCPC-250

Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.


Grégoire Audo

Latest:

Gingerol purification with Centrifugal Partition Chromatography SCPC-250

Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.



Viatcheslav Artaev

Latest:

A New Path to High-Resolution HPLC–TOF-MS — Survey, Targeted, and Trace Analysis Applications of TOF-MS in the Analysis of Complex Biochemical Matrices

High performance time-of-flight mass spectrometry (TOF-MS) is applied to the analysis and characterization of complex biological samples. High mass accuracy, high resolution, and accurate relative isotope abundance are all applied to the determination of analytes covering a range of concentrations in complex matrices including plasma and urine. Qualitative and quantitative evaluations are provided and include demonstrations of the impact of high-performance MS on sensitivity and selectivity. The ability to leverage high-performance MS in conjunction with a broad dynamic range in rapid ultrahigh-pressure liquid chromatography (UHPLC) analyses to identify unknowns and to propose putative metabolic biomarkers is demonstrated. The impact of speed of analysis and selectivity to the depth of coverage and accuracy of the analyses are discussed.


ELGA LabWater

Latest:

Ultrapure Water for HPLC and UHPLC

It is important for the standards, organic solvents, and HPLC mobile phases to be of the highest purity, but water quality is often taken for granted.



Daniel Eßer

Latest:

A Study on the Optimization of the Ion-Pair Reversed Phase Liquid Chromatography Analysis of Protected And Unprotected DNA Oligonucleotides

This article describes a comprehensive study on optimizing ion-pair reversed-phase liquid chromatography for analysing protected and unprotected single-stranded DNA oligonucleotides.









Lisa A. Holland

Latest:

Self-Assembled Nanomaterials for Enhanced Chemical Separations

Nanomaterials offer exciting possibilities for improving separations with techniques such as TLC, SPME, and CE.


M. McCullagh

Latest:

Authentication and Routine Screening of Ginsenoside Isomers in Functional Food Products: UHPLC Coupled with Ion Mobility Mass Spectrometry

Ginseng has been used worldwide for thousands of years. Thought to possess therapeutic effects, it has been marketed as a natural product for the treatment of disease. This article describes how ultrahigh-performance liquid chromatography (UHPLC) can be coupled with ion mobility mass spectrometry (IMS-MS) to profile phytochemicals contained within ginseng and confirm quality.


D. Douce

Latest:

Authentication and Routine Screening of Ginsenoside Isomers in Functional Food Products: UHPLC Coupled with Ion Mobility Mass Spectrometry

Ginseng has been used worldwide for thousands of years. Thought to possess therapeutic effects, it has been marketed as a natural product for the treatment of disease. This article describes how ultrahigh-performance liquid chromatography (UHPLC) can be coupled with ion mobility mass spectrometry (IMS-MS) to profile phytochemicals contained within ginseng and confirm quality.



Corey D. Broeckling

Latest:

Nontargeted Metabolite Profiling in Next-Generation Plant Breeding: A Case Study in Malting Barley

The power of nontargeted metabolite profiling is illustrated in a study focused on the determination of molecular markers in malting barley that are predictive of desirable malting quality for brewing applications. The metabolite extraction, detection, and analysis methods are high throughput and reproducible, and therefore, this approach represents a practical addition to the plant breeder’s molecular toolbox.



Noelia Belmonte-Valles

Latest:

Screening for Hundreds of Pesticide Residues Using a GC/Q-TOF with an Exact Mass Pesticide Database in Food

This note discusses how to rapidly screen, identify, and generate quantitative information for pesticide residues in food with the Agilent 7200 GC/Q-TOF with Agilent MassHunter Qualitative Analysis All Ions workflow.



B. M. Wagner

Latest:

Tools to Improve Protein Separations

The capability to separate and analyze a wide range of proteins in complex systems remains a prime requirement in the biochemical sciences. Intact protein separations are especially difficult as these large molecules can present different conformations, association states and amphoteric features with chromatographic surfaces. Combining high performance liquid chromatography (HPLC) and ultrahigh pressure liquid chromatography (UHPLC) with mass spectrometry (MS) has proven to be an effective approach for solving difficult problems involving protein analyses. Considerable effort has been made to develop columns for separating proteins with high efficiency for reversed-phase, ion-exchange, size-exclusion chromatography, hydrophilic interaction liquid chromatography (HILIC), and hydrophobic interaction chromatography (HIC). Even so, many situations still exist where insufficient resolution is available for accurate protein analysis even when high-resolution MS is available. This presentation provides a brief overview of new approaches being investigated in the author's laboratories for obtaining superior protein separations. This includes new approaches for obtaining better protein separations with columns of highly-efficient superficially porous silica particles and techniques using MS-friendly mobile phases with effective methods for changing protein selectivity (band spacings) by column type and organic mobile phase modifiers.


D. R. Nehring

Latest:

Tools to Improve Protein Separations

The capability to separate and analyze a wide range of proteins in complex systems remains a prime requirement in the biochemical sciences. Intact protein separations are especially difficult as these large molecules can present different conformations, association states and amphoteric features with chromatographic surfaces. Combining high performance liquid chromatography (HPLC) and ultrahigh pressure liquid chromatography (UHPLC) with mass spectrometry (MS) has proven to be an effective approach for solving difficult problems involving protein analyses. Considerable effort has been made to develop columns for separating proteins with high efficiency for reversed-phase, ion-exchange, size-exclusion chromatography, hydrophilic interaction liquid chromatography (HILIC), and hydrophobic interaction chromatography (HIC). Even so, many situations still exist where insufficient resolution is available for accurate protein analysis even when high-resolution MS is available. This presentation provides a brief overview of new approaches being investigated in the author's laboratories for obtaining superior protein separations. This includes new approaches for obtaining better protein separations with columns of highly-efficient superficially porous silica particles and techniques using MS-friendly mobile phases with effective methods for changing protein selectivity (band spacings) by column type and organic mobile phase modifiers.