Author


Megan McGuigan

Latest:

Pesticide Analysis in Dietary Supplements Using Gas Chromatography Time-of-Flight Mass Spectrometry

Screening for pesticide content is a common analysis that is done in laboratories where many samples need to be analyzed over a short period of time. The desire for a high-speed analysis is due to the need for a fast result from a potentially large volume of samples. A fast analysis is typically achieved through the use of high carrier gas flow rates in combination with temperature programming. A fast detector is needed in order to fully characterize the narrow chromatographic peaks generated from these experiments. TOFMS is ideal for detecting these narrow peaks because of its fast acquisition rate over a full mass range at all times during the experiment. In addition, TOFMS provides for non-skewed mass spectra making peak deconvolution possible.


Hlanganani Tutu

Latest:

Extraction and Preconcentration of Residues and Contaminants in Food Samples Using Membrane Techniques

Guest authors from South Africa review the application of membranes in the extraction, preconcentration, and separation of various contaminants in food.



Sandra M. Lorenz

Latest:

Stability of Formic Acid in Methanol Solutions and the Implications for Use in LC–MS Gradient Elution Analysis

Dilute formic acid solutions in methanol were found to decline in acid content with time, the extent of the decline depending upon the initial amount of water present in these solutions. The effect of the formic acid concentration change upon the separation of peptides using high performance liquid chromatography (HPLC) is examined briefly.


William Long

Latest:

Low and High pH Stability of an Agilent Poroshell HPH

HPLC column stability is a critical factor. This note describes how the Agilent Poroshell HPH-C18 column performed brilliantly in an elevated pH mobile phases such as ammonium bicarbonate buffer.


Dave Thomas

Latest:

Analytical Methods to Characterize and Quantify PEG and PEGylated Biopharmaceuticals

Biotherapeutic peptides and proteins are often PEGylated (covalently bonded to polyethylene glycol polymers) to improve bioavailability, reduce immunogenicity, and extend circulating half-life (1). Achieving the desired properties for each application depends on optimizing the number and site of polymers attached, chain length, and the degree of chain branching.


L. C. Short

Latest:

Atmospheric Pressure Photoionization — The Second Source for LC-MS?

This article explores the progress that atmospheric pressure photoionization (APPI) has made in its relatively short history for LC–MS analysis. Specifically, the authors examine the combination of APPI and electrospray ionization (ESI).



Srinivasa Rao

Latest:

Bioinert Versus Biocompatible: The Benefits of Different Column Materials in Liquid Chromatography Separations

In this study, we compare the performance of plastic and metal materials in UHPLC columns designed for the analysis of biological molecules. We evaluate the performance of these materials in terms of inertness, column chromatographic performance, and reproducibility.


James Neal-Kababick

Latest:

GC–MS Screening for Melamine Adulteration in Baby Formula and Dairy Products

Melamine is an industrial chemical with a high nitrogen content that can cause kidney stones and lead to renal failure. In some instances, melamine has been added to baby formula and dairy products as a substitute for protein. This paper presents an efficient and definitive gas chromatography–mass spectrometry (GC–MS) method to identify melamine and related compounds based on the released US Food and Drug Administration (FDA) method.


Jasco, Inc.

Latest:

Reproducibility of Retention Times and Peak Area in a UHPLC System (X-LC®)

With recent advances in technology and the use of ultra high pressure chromatography systems becoming more commonplace, it is of use to compare reproducibility of retention time and peak area of UHPLC vs HPLC.


Stéphane Mabic

Latest:

The Misunderstood Laboratory Solvent: Reagent Water for HPLC

A good pretreatment system combines reverse osmosis and electrodeionization technologies.


JEOL USA, Inc.

Latest:

msFineAnalysis AI for GC-Alpha

This application note provides an overview of JEOL’s innovative msFineAnalysis AI and its capabilities for enhancing GC-MS analysis.


Eric Denoyer

Latest:

Reduced Solvent Usage and Increased Throughput for PAH Analysis Using UHPLC

Polynuclear aromatic hydrocarbons (PAHs) are carcinogenic condensed ring aromatic compounds widely found as trace pollutants in waters, wastes, air particulates, soil and foods. PAHs can be routinely monitored using HPLC with a combination of UV and fluorescence detection as prescribed in EPA methods 550.1, 610 and 8310.


Chris Cantelmo

Latest:

Fast Gradient LC Using Your Existing Instrumentation

Transforming "standard" gradient HPLC systems into extremely fast gradient systems is readily achievable with proper application of chromatographic principles, particularly temperature control, combined with utilization of advanced HPLC columns. Bottom line: You don't have to buy a new LC to achieve ultra high quality and speed!


Monica Dolci

Latest:

Rapid Separation of Rhodamines

This application note describes a rapid U-HPLC method for the separation of sulphorhodamine 101 (Texas Red®) and its three water-soluble derivatives using a Hypersil GOLD™ column packed with 1.9 μm particles.


Jennifer Peterson

Latest:

Automated Extraction Technique for Improved Recovery of Phenols

Dionex has developed a new standard for flow-through solvent extraction which allows accelerated solvent extraction (ASE®) of matrices that have undergone acid or alkaline pretreatment or digestion. The new ASE 150 and ASE 350 systems use extraction cells and post-cell solvent pathways constructed of Dionium™ material. This pH-hardened substance resists corrosion under acidic or alkaline conditions used in standard pretreatments, widening the scope of ASE applications and significantly expanding its capabilities.


Bill Schnute

Latest:

IC–MS Analysis of Low Molecular Mass Organic Acids in Beverages

Organic acids are present in many matrices and play crucial roles. Extensive research involving low molecular mass organic acids (LMMOA) has been performed in food chemistry since these acids contribute to the organoleptic properties of food and beverages, including flavor, color, aroma, taste, shelf-life, and health effects. Developing a profiling method to monitor LMMOA levels in raw materials and final products is very desirable. Many reported methods focus on limited numbers of LMMOAs and are incapable of providing a complete LMMOA profile.


Jan Poustka

Latest:

Analysis of Deoxynivalenol in Beer

Mycotoxins, toxic secondary metabolites of several fungal species, represent food safety issues of high concern. Deoxynivalenol, the most abundant trichothecene mycotoxin, can be found worldwide as a contaminant of wheat, barley, maize and other cereals (1,2). The transmission of deoxynivalenol from barley into beer has been reported in several studies (3,4). Therefore, its levels should be controlled.


Carl Sanchez

Latest:

Development of a High-Throughput LC–MS Assay for Drugs of Abuse from Biological Matrices

A high-throughput LC–MS method using core-shell UHPLC columns to screen for a panel of 11 drugs of abuse (expanded SAMHSA) was developed. The corresponding SPE method allowed the reproducible separation and quantitation of these 11 components in less than 2 min. This method demonstrates the power of new-generation HPLC media as well as some of the factors one must consider when developing such methods for LC–MS analysis.



Jeffrey Zonderman

Latest:

Quantifying Smoke Taint in California Wines by Immersive Sorbent Sheet Extraction Prior to Direct Analysis in Real-Time Mass Spectrometry (DART-MS)

Measuring volatile phenols in wine is essential in ensuring superior wine quality. A new analytical technique, called solid-phase mesh-enhanced sorption from headspace (SPMESH), was modified with direct immersion (DI) conditions and coupled to direct analysis in real time–mass spectrometry (DART–MS) to be used to detect smoke taint in winemaking.


Petr Jandik

Latest:

Determination of Phenolic Compounds Using HPLC and Electrochemical Detection with Disposable Carbon Electrodes

Phenols are frequently present in water because of their widespread use in commercial products and because they are by-products of processes in petrochemical, pulp and paper, plastic, and glue manufacturing industries (1,2). The concentration of phenolic compounds in the waste discharges can be as high as 20 mg/L (2); however, phenol-containing pesticides and wood preservatives may cause significant health hazards even at mg/L levels (1). Consequently, it is important to monitor phenols and substituted phenols in environmental and biological samples. Liquid chromatography with electrochemical detection is one of the widely used methods due to its high selectivity and sensitivity for phenolic compounds. However, glassy carbon working electrodes, used in the electrochemical detection of phenols, often require polishing (3). This time-consuming and often poorly reproducible polishing can be avoided with disposable carbon electrodes, which offer comparable or better analytical performance (4).


Stephen Naylor

Latest:

Inside the Personalized Medicine Toolbox: GCxGC-Mass Spectrometry for High-Throughput Profiling...

The authors present results that suggest that high-throughput, high-coverage profiling capabilities, such as those afforded by GCxGC-TOF-MS, can impact the development of personalized medicine.


Kunihiro Kishida

Latest:

Determining Zeranol in Bovine Tissues Under Nontoxic Conditions

The authors have developed a method of determining zeranol residues in bovine tissues without using toxic chemicals, organic solvents, and reagents in sample preparation and reversed-phase HPLC separation.


Jeffrey S. Rohrer

Latest:

Determination of Hexavalent Chromium Cr(VI) in Drinking Water by Suppressed Conductivity Detection

Development of an ion chromatography (IC) method using suppressed conductivity detection for the determination of Cr(VI) in drinking water.


Pauline Vollmerhaus

Latest:

Challenges in Small-Molecule Quantitation by Mass Spectrometry

Drug discovery scientists are continually striving to improve productivity and efficiency in their workflows. From early discovery to clinical development, existing workflow bottlenecks represent an opportunity to develop solutions to speed the process and improve productivity. The key requirements for quantitative analysis are precision, accuracy, and linear dynamic range. With any quantitative instrument, the hope is that it will be applicable to a vast range of coumpounds, ruggest, and fast. New mass spectrometry (MS) technologies are being developed that meet these criteria and permit high throughput while enabling its application to areas in which speed limitations previously curtailed its practicality. In particular, in the area of ADME profiling, new MS platforms are becoming available that increase the throughput by at least 25-fold, by combining the speed of matrix-assisted laser desorption ionization (MALDI) with the specificity of triple-quadrupole MS. This is bound to greatly accelerate the ADME..


Clarence Wentzel

Latest:

Performance Gains Using Hydrogen Carrier Gas in Refinery Gas Analysis

Escalating costs and increasing demands for helium, coupled with diminishing helium supply, have given rise to the investigation and use of hydrogen as an alternate choice of carrier gas for use in gas chromatography (GC) applications. This application note illustrates that the use of hydrogen as a carrier gas as an alternative to helium in refinery gas applications is not only possible, but also results in improved performance and higher sample throughput.


Stephen J. Valentine

Latest:

Inside the Personalized Medicine Toolbox: GCxGC-Mass Spectrometry for High-Throughput Profiling...

The authors present results that suggest that high-throughput, high-coverage profiling capabilities, such as those afforded by GCxGC-TOF-MS, can impact the development of personalized medicine.


Scott Silver

Latest:

Analysis of Hydrophilic Molecules in Biological Fluid with Novel Mixed-Mode Column

In pharmaceutical development, it is important to analyze small molecules or their metabolites in biological fluids. For this purpose, the analytical methods such as sample pretreatment, 2D-LC and LC–MS have been developed. However there are still problems of resolution and protein adsorption. As a result, satisfying analytical results have not always been achieved.