Author


Stepan Podzimek

Latest:

The Benefits of Multi‐angle Light Scattering for Size‐Exclusion Chromatography

Analytical size-exclusion chromatography (SEC) often suffers from several limitations. This article shows how some of these limitations can be partly (or even completely) resolved by a multi-angle light scattering (MALS) detector for the determination of molar mass distributions of synthetic and natural polymers.


Dr Alessandro Casilli

Latest:

Analysis of Allergens in Fragrances using Multiple Heart-cut Multidimensional Gas Chromatography–Mass Spectrometry

A total of fourteen cuts were defined because the retention time difference between several skin sensitizors was only slight.


M. Alexander Shaw

Latest:

Investigation of Transferrin Structure via Novel Electron Capture Dissociation Techniques Using a Hybrid Linear Ion Trap Time-of-Flight Mass Spectrometer

Protein and peptide analysis via tandem mass spectrometry (MS-MS) has resulted in a wealth of information regarding protein identification, structure, and abundance levels over the past 10 years. Techniques such as neutral loss scanning and collision-induced dissociation (CID) have been especially helpful in facilitating the identification of a multitude of previously unknown sites of protein phosphorylation. However, many of the techniques used to obtain this information are labor intensive and work inconsistently. To address this problem, much effort has been put forth to find alternative methods of fragmenting peptides and proteins that are less difficult and applicable to a wide gamut of peptide classes. Examples of recently developed dissociation techniques include infrared multiphoton dissociation (IRMPD) and electron transfer dissociation (ETD). The implementation of these new techniques has widened the spectrum of peptides amenable to tandem mass spectral analysis.


L.H. Reyes

Latest:

Evaluation of Extraction Methods and Bias Correction by EPA Method 6800 Protocol for Mercury Species in Tuna Fish Tissue Using an Ion Chromatograph Coupled to an ICP–MS

Mercury pollution mainly originates from industrial activities such as chlorine production, garbage incineration and above all coal-fueled power generation. The US Environmental Protection Agency (US EPA) considers mercury as highly toxic with a pronounced accumulative and persistent character.


Judd Aiken

Latest:

Facilitating Discovery of Prion Disease Biomarkers by Quantitative Glycoproteomics

The authors examine MS-based methods to search for protein biomarkers of prion diseases from plasma samples.



Dan DiFeo

Latest:

The Integration of Microextraction Packed Sorbent (MEPS) into Multidimensional Stratagies

LC–GC approaches to analysis are particularly attractive because they combine the selectivity of solid phase sorbents in the first dimension with the separating power and peak capacity of a capillary GC column in the following dimensions. Their widespread use is limited because of the difficultly in desolvating the stream from the LC dimension without the solvent vapour passing down the GC column in significant quantity. An alternative approach to elution chromatography in the first dimension is to harness the specificity of the solid-phase process for digital chromatography using discontinuous changes in solvent polarity. Digital chromatography on a small sorbent bed reduces the volume of mobile phase to discrete plugs that are sufficiently small to be injected directly into a GC with a large volume injector or, alternatively, subsampled into a conventional split/splitless injector.


John Heim

Latest:

Comprehensive Analysis of Human Plasma Using Gas Chromatography–High Resolution Time-of-Flight Mass Spectrometry: A Workflow to Leverage Electron and Chemical Ionization

Gas chromatography with electron ionization and mass spectrometry (GC–EI-MS) detection is a workhorse among analytical techniques in metabolomics. A major challenge in the utilization of GC–EI-MS in metabolomics is the identification of unknowns.



Andrew G. Mckee

Latest:

Inside the Personalized Medicine Toolbox: GCxGC-Mass Spectrometry for High-Throughput Profiling...

The authors present results that suggest that high-throughput, high-coverage profiling capabilities, such as those afforded by GCxGC-TOF-MS, can impact the development of personalized medicine.


Liming Peng

Latest:

Enantiomeric Separation of Proton Pump Inhibitors Using Polysaccharide-Based Chiral Stationary Phases in Reversed-Phase HPLC Conditions

The chiral analysis and purification of proton pump inhibitors (PPIs) has become a popular topic as more of these drugs fall out of patent protection each year. Due to their wide range of enantiomeric selectivity, a set of polysaccharide-based chiral stationary phases (CSPs) was screened to identify methods for the successful enantioseparation of four benzimidazoles; rabeprazole, lansoprazole, omeprazole, and pantoprazole.


David Weil

Latest:

The Use of Accurate Mass and MS-MS for the Analysis of PPCPs in Water

The United States Geological Survey (USGS) has found pharmaceuticals and personal-care products (PPCPs) containing known or suspected endocrine-disruptors in U.S. rivers. As such, it is important to use adequate techniques to help identify these compounds and possible metabolites.


Brian Shofran

Latest:

Metabolic Profiling of Peanut Plant Material by LC–TOFMS

There has been an increasing interest in the presence and availability of compounds in plant materials that may possess bioactive properties, in particular, antioxidant activity. Some of these compounds have been attributed to possess anticancer, antiaging, and antimutagenic properties as well as other health benefits (1). The types of plants that have been investigated cover a vast range from common foodstuffs to regional or exotic materials. Plant parts under study have included portions that are traditionally known to be edible, as well as sections that are considered "waste" or used for animal forage. Because most screening techniques involve lengthy separations, high throughput HPLC methods are desirable.


Anthony R. Kemperman

Latest:

Stability of Formic Acid in Methanol Solutions and the Implications for Use in LC–MS Gradient Elution Analysis

Dilute formic acid solutions in methanol were found to decline in acid content with time, the extent of the decline depending upon the initial amount of water present in these solutions. The effect of the formic acid concentration change upon the separation of peptides using high performance liquid chromatography (HPLC) is examined briefly.


Philip Wylie

Latest:

Comprehensive Screening, Confirmation, and Quantification of Organic Pesticides in Foods by GC–MS and LC–MS

With the globalization of trade, food production and distribution have become truly international businesses. When we dine out, the fish might come from Japan, the rice from Australia, the spices from China, and the strawberries from Mexico. We take it for granted that the food we eat is safe and free from contamination that could make us seriously ill.


Michael H. Coan

Latest:

Chromatography Applications in Drug Discovery of Therapeutic Proteins

In this month's installment of "Directions in Discovery," the authors discuss how, with the arrival of combinatorial libraries and high-throughput screening, pharmaceutical firms can develop new models of drug discovery that not only lessen the initial capital outlay involved in drug discovery, but also refine the discovery process.


Robert F. Wallace

Latest:

Method 6040D, Odors in Drinking Water, Using SPME on the Supelco SLB-5ms Capillary Column

A method for trace odor components, isopropyl-methoxypyrazine (IPMP), isobutyl-methoxypyrazine (IBMP), methylisoborneol (MIB), and geosmin in drinking water involving the use of solid phase microextraction (SPME) and the SLB-5ms capillary column using gas chromatography/mass spectrometry (GC–MS).


William Campbell

Latest:

Ascentis® Express RP-Amide Expands the Selectivity of Fused-Core ™ Particle Technology HPLC Columns

The Fused-Core particle consists of a 1.7 micron solid core and a 0.5 micron porous shell yielding a 2.7 micron diameter. One of the benefits of the Fused-Core particle is the small diffusion path (0.5 microns) compared to conventional fully porous particles. The shorter diffusion path minimizes peak broadening. In fact, there have been many reports on the vast improvements in efficiency provided by Fused-Core particles versus conventional particles. These improvements provide sub-2 micron like performance at half of the backpressure allowing Ascentis Express columns to be used in conventional HPLC as well as UHPLC systems.


Ian J. Garrard

Latest:

Dynamic Extraction: A High-Speed, High-Capacity Purification Process That is Rapidly Scalable

The authors discuss a preparative process using the principles of countercurrent chromatography. This process is faster, capable of loadings from milligrams to hundreds of grams, and uses robust equipment.


Toni Ann Riveros

Latest:

Chemometrical Experimental Design-Based Optimization Studies in Capillary Electrophoresis Applications

A synopsis of our work detailing the use of chemometric response surface methodology (RSM) in two capillary electrophoresis (CE) studies is described.


Petr Lebeda

Latest:

Asymmetric Flow Field Flow Fractionation: A Powerful Method for Polymer Characterization

Recent development of the instrumentation for asymmetric flow field flow fractionation (FFF) brings new possibilities for the characterization of synthetic and natural polymers with several advantages over traditionally used size exclusion chromatography (SEC). The main difference of asymmetric flow FFF compared to SEC is that the polymer separation takes place in an entirely empty channel, which eliminates undesirable SEC effects such as shearing degradation of polymers with ultra high molar mass, anchoring of branched macromolecules in SEC column packing, and enthalpic interactions of polymer molecules with a stationary phase.


Hassan Y. Aboul-Enein

Latest:

Fast Analysis of Third-Generation Cephalosporins in Human Plasma by SPE and HPLC Methods

A fast, selective, and reproducible high performance liquid chromatography (HPLC) method was developed and validated for the analyses of third-generation cephalosporin antibiotics, namely, ceftriaxone, cefixime, and cefdinir in human plasma. The analysis was carried out on a 150 mm Ã- 4.6 mm, 5.0-µm C18 column. The mobile phase used was 80:20 (v/v) 50 mmM phosphate buffer (pH 5.0)–methanol at a flow rate of 1.0 mL/min with 230-nm UV detection.


Robert F. Bonsall

Latest:

Extraction and Detection of Antibiotics in the Rhizosphere Metabolome

Root diseases caused by soilborne plant pathogens are responsible for billions of dollars of losses annually in food, fiber, ornamental, and biofuel crops. The use of pesticides often is not an option to control plant diseases because of economic factors or potential adverse effects on the environment or human health. For this reason, many Americans are now buying pesticide-free organic foods. Organic agriculture has few options for controlling pests and thus must make full use of natural microbial biological control agents in soils that suppress diseases.


Robert Montgomery

Latest:

ICP MS Detection for HPLC Analyses of Pharmaceutical Products

The authors investigate applications for ICP-MS detection with reversed-phase HPLC.


Jay Corr

Latest:

Challenges in Small-Molecule Quantitation by Mass Spectrometry

Drug discovery scientists are continually striving to improve productivity and efficiency in their workflows. From early discovery to clinical development, existing workflow bottlenecks represent an opportunity to develop solutions to speed the process and improve productivity. The key requirements for quantitative analysis are precision, accuracy, and linear dynamic range. With any quantitative instrument, the hope is that it will be applicable to a vast range of coumpounds, ruggest, and fast. New mass spectrometry (MS) technologies are being developed that meet these criteria and permit high throughput while enabling its application to areas in which speed limitations previously curtailed its practicality. In particular, in the area of ADME profiling, new MS platforms are becoming available that increase the throughput by at least 25-fold, by combining the speed of matrix-assisted laser desorption ionization (MALDI) with the specificity of triple-quadrupole MS. This is bound to greatly accelerate the ADME..


Tim Shelbourn

Latest:

ICP MS Detection for HPLC Analyses of Pharmaceutical Products

The authors investigate applications for ICP-MS detection with reversed-phase HPLC.


Agilent Technologies, Inc.

Latest:

Advanced Column Selection and Method Optimization for Biomolecular Purification and Characterization

Webinar Dates/Times: Tuesday, December 10, 2024 7am PST | 10am EST | 3pm GMT | 4pm CET


Christine Hunter

Latest:

A New Perspective on the Challenges of Mass Spectrometry

For drug discovery workflows, the issue of metabolite detection and identification in in vivo systems is a critical challenge. The wide range of complex matrices (such as bile, plasma, urine, and fecal extracts), and the ion suppression effects of these biological fluids, can cause a severe decrease in the ability to detect metabolites. Greater instrument sensitivity is necessary to detect these compounds and, at the same time, helps to minimize sample preparation, simply diluting the negative effects of these complex matrices and avoiding the time- and labor-consuming sample cleanup or concentration steps that otherwise might be required.


Stacy Henday

Latest:

Rapid Determination of Azo Dyes from Textile Samples Using Dionex ASE and U3000 HPLC-UV Instruments with MSQ Plus Detection

Azo dyes are used widely in the manufacture of various consumer goods such as leather, textiles, plastics, paper, hair care products, and cosmetics. On September 11, 2003, the European Union enacted European Parliament Directive 2002/61/EC, prohibiting the manufacture and sale of consumer goods containing specified azo dyes (1). The azo dyes of concern are those that can be reduced to aromatic amines. There are 22 aromatic amines classified as carcinogenic or potentially carcinogenic to humans.


Steve Jordan

Latest:

Removal of Endogenous Matrix Components Using EVOLUTE® CX Mixed-Mode Cation Exchange Solid Phase Extraction

Sample preparation is an essential technique to remove unwanted matrix components prior to LC–MS-MS analysis of drugs in biological fluids. Plasma matrix components whether endogenous (salts, proteins, and phospholipids) or exogenous (dosing vehicles, e.g. PEG 400), can interfere with compounds of interest leading to regions of ion suppression or enhancement. This can lead to inaccurate quantitation and have adverse effects on sensitivity. Mixed-mode SPE provides cleaner extracts as a result of rigorous interference wash steps, afforded by the dual retention mechanism of the sorbents.