Author


Julie Wingate

Latest:

GSH Conjugate Metabolite Identification with a Hybrid Triple- Quadrupole–Linear Ion-Trap Instrument and Automated Software-Driven Method Development

In drug discovery, determining information about the extent of metabolism and the elucidation of metabolite structures is a vital step for lead optimization and drug scaffold refinement. The identification and characterization of metabolites plays an important role in both the drug discovery and development phases, as unsuitable pharmacokinetics (bioavailability and drug distribution), toxicity, and adverse drug reactions might be linked to metabolic instability. Historically, metabolite identification was carried out after a compound had been chosen for drug development. However, to reduce candidate failures attributed to toxicity effects, many pharmaceutical companies now conduct these experiments in the earliest phases of candidate drug selection.


Restek Corporation

Latest:

Guard Columns Unleashed: Their Importance in HPLC

Webinar Date/Time: Thu, Nov 14, 2024 10:00 AM EST


Ming Gu

Latest:

Enhancing Mass Spectral Formula Determination by Heuristic Rules

A new approach to enhancing the performance of formula identification of true unknowns beyond high mass and spectral accuracy was evaluated. Three heuristic rules on upper limits and ratios of elements were tested for their effectiveness in filtering out false positive formulas with both high- and low-resolution mass spectrometry data. The rule on elements' upper limits was found to be the most effective one in eliminating incorrect formulas.


Geoffrey B. Cox

Latest:

Reversed-Phase Enantioselective Chromatography with New µ-mm Chiral Stationary Phases

To meet the growing need for fast reversed-phase enantiomer separations, two new 3-μm reversed-phase columns, CHIRALCEL® OD® -3R and CHIRALPAK® AD® -3R, have been introduced. High column performance and column stability under a wide range of conditions, including aqueous solvent systems suited to LC–MS, have been


Joe Binkley

Latest:

Comprehensive Screening of Pollutants in Household Dust Using High-Resolution Mass Spectrometry with Enhanced Chromatographic Resolution

High resolution time-of-flight mass spectrometry (HR-TOF-MS) with a novel multimode ionization source together with enhanced chromatographic resolution can successfully detect and identify pollutants in household dust samples. Here’s how.


Shahana Wahab Huq

Latest:

Novel Sample Preparation Methods for Analyzing Pain Management Drugs in Complex Matrices

The importance of sample preparation for analyzing pain management drugs in different matrices


Shirley Liu

Latest:

Metabolite Identification Using Multiple Mass Defect Filters and Higher Energy Collisional Dissociation on a Hybrid Mass Spectrometer

In this article, the authors evaluate the use of multiple mass defect filters on metabolite identification data from a hybrid mass spectrometer. The study also investigates the use of higher energy collisional dissociation for structural elucidation in metabolite identification experiments.


Richard Verseput

Latest:

A Quality-by-Design Methodology for Rapid LC Method Development, Part III

The guest columnists continue their examination of how statistically rigorous QbD principles can be put into practice.


Chongyu Shen

Latest:

LC–MS-MS Determination of Malachite Green and Leucomalachite Green in Fish Products

Although not currently used in U.S. or European aquaculture, malachite green (MG) is still an effective and inexpensive fungicide that is used in other countries, particularly in Asia. During metabolism, MG reduces to leucomalachite green (LMG) (Figure 1), which has been shown to accumulate in fatty fish tissues. Trace levels of MG and LMG residues continue to be found in fish products. In a 2005 report, MG was found in 18 out of 27 live eel or eel products imported from China to Hong Kong local market and food outlets, resulting in a government recall and destruction of all remaining products (1).



Nelu Grinberg

Latest:

Sulfated β-Cyclodextrin as Chiral Mobile Phase Additive for Ultrahigh-Pressure Liquid Chromatography

A fast enantiomeric separation of a chiral aromatic amine was achieved, using ultra high pressure liquid chromatography and highly sulfated β-cyclodextrin (S-β-CD) as a chiral additive in the mobile phase. The stationary phase consisted of a core shell support with a particle size of 2.7 µm. Under these conditions the baseline separation was obtained within 2.5 min. The influence of the concentration of the additive, along with the thermodynamics of the separation, were studied. Molecular mechanics calculations were consistent with the experimental data for the order of elution, providing further evidence of these interactions. The enantiomeric separation at high temperature (90 °C) using only water as mobile phase also was achieved for the first time.


Hiroyuki Moriyama

Latest:

Fast Analysis of Charge Variants of Monoclonal Antibodies by Ion Exchange Chromatography

Development and production of biopharmaceuticals is a growing segment of the pharmaceutical industry. The development of vaccines is only one - but as a result of the current H1N1 flu hysteria it is the most striking - segment of this emerging field. Recombinant proteins, ranging from insulin products to therapeutic antibodies, represent another important group of biopharmaceuticals. With the introduction of the first so-called biosimilars in Europe the demand for highly efficient analysis methods is further increasing. Newly developed stationary phases for the typical modes of biochromatography of native proteins such as SEC or IEC open up new opportunities for increasing throughput in the bioanalytical lab.


Monika Kansal

Latest:

Structured Approach to Method Development for Bioanalytical HILIC–MS-MS Applications

A structured, general purpose approach to method development for bioanalytical hydrophilic interaction chromatography–tandem mass spectrometry (HILIC–MS-MS) applications is described.


G.M. Mizanur Rahman

Latest:

Evaluation of Extraction Methods and Bias Correction by EPA Method 6800 Protocol for Mercury Species in Tuna Fish Tissue Using an Ion Chromatograph Coupled to an ICP–MS

Mercury pollution mainly originates from industrial activities such as chlorine production, garbage incineration and above all coal-fueled power generation. The US Environmental Protection Agency (US EPA) considers mercury as highly toxic with a pronounced accumulative and persistent character.


Heinz P. Singer

Latest:

Online Automated SPE–LC–MS-MS Quantitation of Sulfonamide Antibiotics and Pesticides in Surface Waters Using a Triple-Quadrupole Mass Spectrometer and Electrospray Ionization Probe

This article describes a fully automated online solid-phase extraction–liquid chromatography–tandem mass spectrometry (SPE–LC–MS-MS) setup using a mass spectrometer and an electrospray ionization probe for analyzing different groups of polar contaminants in natural waters. The goal was to develop an online SPE method for the quantification of sulfonamide antibiotics, including their acetyl metabolites, as well as for frequently used pesticides (triketones, phenylureas, chloracetanilides, phenoxyacetic acids, amides, and triazines) in ambient waters. The analytical methods were applied successfully for a field study in an agricultural region within the catchment area of Lake Greifensee near Zurich, Switzerland.


PSS Polymer Standards Service GmbH

Latest:

Tips & Tricks: Treating Your GPC/SEC System Properly

This installment of Tips & Tricks will provide some helpful pointers to keep your GPC/SEC system in good shape.


Sheldon Henderson

Latest:

Automated Extraction Technique for Improved Recovery of Phenols

Dionex has developed a new standard for flow-through solvent extraction which allows accelerated solvent extraction (ASE®) of matrices that have undergone acid or alkaline pretreatment or digestion. The new ASE 150 and ASE 350 systems use extraction cells and post-cell solvent pathways constructed of Dionium™ material. This pH-hardened substance resists corrosion under acidic or alkaline conditions used in standard pretreatments, widening the scope of ASE applications and significantly expanding its capabilities.


Yingying Huang

Latest:

Metabolite Identification Using Multiple Mass Defect Filters and Higher Energy Collisional Dissociation on a Hybrid Mass Spectrometer

In this article, the authors evaluate the use of multiple mass defect filters on metabolite identification data from a hybrid mass spectrometer. The study also investigates the use of higher energy collisional dissociation for structural elucidation in metabolite identification experiments.


Derek Fisher

Latest:

Dynamic Extraction: A High-Speed, High-Capacity Purification Process That is Rapidly Scalable

The authors discuss a preparative process using the principles of countercurrent chromatography. This process is faster, capable of loadings from milligrams to hundreds of grams, and uses robust equipment.


Leah Williamson

Latest:

ICP MS Detection for HPLC Analyses of Pharmaceutical Products

The authors investigate applications for ICP-MS detection with reversed-phase HPLC.



Allen Herbst

Latest:

Facilitating Discovery of Prion Disease Biomarkers by Quantitative Glycoproteomics

The authors examine MS-based methods to search for protein biomarkers of prion diseases from plasma samples.


Philippe Lagueux

Latest:

Chemical Warfare Agent Spectral Imaging for Real-Time Identification and Localization

In this article, the authors discuss the need for protection against chemical attacks and the role of passive imaging spectroradiometers in the detection of remote chemical agents.


Todd Gillespie

Latest:

ICP MS Detection for HPLC Analyses of Pharmaceutical Products

The authors investigate applications for ICP-MS detection with reversed-phase HPLC.


Charanjit Saini

Latest:

A New High-Resolution, High-Speed Reversed-Phase Monolithic 1 mm I.D. Column for the Separation of Proteins

The new reversed-phase ProSwift® 1 mm i.d. column is a divinylbenzene-based monolithic column for routine chromatography of proteins and other biomolecules. It is available in two different lengths. The shorter (1 Ã- 50 mm) format is designed for fast separations and the longer (1 Ã- 250 mm) format is intended for high resolution analytical separations. However, depending on the application, either can be used for separation of proteins and for coupling with mass spectrometry.


Chin-Kai Meng

Latest:

Comprehensive Screening, Confirmation, and Quantification of Organic Pesticides in Foods by GC–MS and LC–MS

With the globalization of trade, food production and distribution have become truly international businesses. When we dine out, the fish might come from Japan, the rice from Australia, the spices from China, and the strawberries from Mexico. We take it for granted that the food we eat is safe and free from contamination that could make us seriously ill.



Katherine M. Stenerson

Latest:

Method 6040D, Odors in Drinking Water, Using SPME on the Supelco SLB-5ms Capillary Column

A method for trace odor components, isopropyl-methoxypyrazine (IPMP), isobutyl-methoxypyrazine (IBMP), methylisoborneol (MIB), and geosmin in drinking water involving the use of solid phase microextraction (SPME) and the SLB-5ms capillary column using gas chromatography/mass spectrometry (GC–MS).


Metrohm AG

Latest:

Using Data Pretreatment to Improve Resolution

Did you know that applying the second derivative as a pretreatment to your near-infrared spectral data can help resolve overlapped peaks?


Claire Bramwell

Latest:

GSH Conjugate Metabolite Identification with a Hybrid Triple- Quadrupole–Linear Ion-Trap Instrument and Automated Software-Driven Method Development

In drug discovery, determining information about the extent of metabolism and the elucidation of metabolite structures is a vital step for lead optimization and drug scaffold refinement. The identification and characterization of metabolites plays an important role in both the drug discovery and development phases, as unsuitable pharmacokinetics (bioavailability and drug distribution), toxicity, and adverse drug reactions might be linked to metabolic instability. Historically, metabolite identification was carried out after a compound had been chosen for drug development. However, to reduce candidate failures attributed to toxicity effects, many pharmaceutical companies now conduct these experiments in the earliest phases of candidate drug selection.