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Acrylamide by QuEChERS Extraction with LC–MS-MS Detection
September 1st 2012Acrylamide is a neurotoxic compound classified as a probable human carcinogen and genotoxicant (1). In 2002, researchers in Sweden uncovered the presence of acrylamide in certain fried and baked foods at relatively high levels (2).
Rapid and Sensitive Determination of Biofuel Sugars
September 1st 2012Biofuels have emerged as an attractive alternative to fossil fuel. Biofuel refers to any fuel that is derived from biomass (typically, plant and animal waste). A common feedstock for bioethanol production is corn stover (leaves and stalks of maize plants left after harvesting). Corn stover processing involves acid treatment to release a mixture of water-soluble carbohydrates, followed by enzymatic reactions to convert the sugars to ethanol.
The Scherzo Family Columns Effectively Separate Pharmaceutical Compounds
September 1st 2012Pharmaceutical compounds are often difficult to analyze on C18 columns alone because they may be charged or have very similar structures. Imtakt Corporation has introduced a family of multimode columns that are able to overcome these separation challenges: Scherzo SS-C18, Scherzo SM-C18, and Scherzo SW-C18, containing C18, anion, and cation ligands.
Analysis of phosphate compounds with the Agilent 1260 Infinity Bio-inert Quaternary LC System
September 1st 2012This application note shows that unspecific reaction of adenosine triphosphate can be completely prevented due to the iron- and steel-free design using the Agilent 1260 Infinity Bio-inert Quaternary LC System.
70-min Amino Acid Analysis of Physiological Samples
September 1st 2012Pickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Post-column derivatization with Ninhydrin offers unmatched selectivity and reproducibility of the analysis for most challenging matrixes.
Determination of Sulfur in Natural Gas by ASTM Method D 6228-11
September 1st 2012Natural gas and other gaseous fuels contain varying amounts and types of sulfur compounds which can be corrosive to equipment and can inhibit or destroy gas processing catalysts. Small amounts of sulfur odorants are added to natural gas and liquefied petroleum gases (LPGs) for safety purposes. Accurate measurement of sulfur species ensures proper process operation and odorant levels for public safety.
Simplified Yet Sensitive Determination of Aniline and Nitroanilines
September 1st 2012Aniline is an organic compound widely used in the polymer, rubber, pharmaceutical, and dye industries. Aniline and its derivatives (e.g., nitroanilines) are suspected carcinogens and are highly toxic to aquatic life. Because these compounds are thermolabile and polar, the traditional analytical methods require a derivatization step prior to GC analysis.
Fast Screening Methods for Steroids by HPLC with Agilent Poroshell 120 Columns
September 1st 2012Selectivity is the most powerful tool to optimize separations in HPLC. This parameter is changed by using different bonded phases or by changing the mobile phase. In this work, Poroshell 120 columns with highly efficient 2.7 ?m superficially porous particles and the Agilent 1260 Infinity Series LC Multi-Method Solution were used to quickly evaluate method development choices for the analysis of steroids.
Molecular Weight Determination of LMWH SEC–MALS vs. SEC–UV-RI
September 1st 2012Low-molecular-weight heparins (LMWHs) are obtained by fractionation or depolymerization of natural heparins. They are defined as having a mass-average molecular weight of less than 8000 and for which at least 60% of the total weight has a molecular mass less than 8000.
Analysis of Intact Proteins on a Thermo Scientific Accucore 150 C4 HPLC Column
September 1st 2012This application note demonstrates the analysis of intact proteins using a Thermo Scientific Accucore 150-C4 (150 ? pore diameter) HPLC column. Analysis of six proteins ranging in mass from 6 to 45 kDa is carried out in 15 min with pressures compatible with conventional HPLC instrumentation.
Creation of a New IMS Analyzer Platform with High Resolving Power
September 1st 2012Ion mobility spectrometry (IMS) has become widely accepted for the detection of chemical warfare agents, explosives, and narcotics as well as for pharmaceutical quality control and pesticide screening of food. Most currently available commercial IMS units have a resolving power of 10–60.
Unique Effects of Two Mobile Phase Alcohols on the RegisPack CLA-1 Chiral Stationary Phase (CSP)
September 1st 2012Typical chiral stationary phase (CSP) screening paradigms utilize a single alcohol component as co-solvent in its mobile phase. Recent unexpected observations have demonstrated that mixed-alcohol mobile phases can enhance or even introduce peak resolution when none existed in a mono-alcohol system.
Fast Petroleum Hydrocarbons Analysis for Underground Storage Tank Monitoring
September 1st 2012Underground storage tanks (USTs) for various petroleum-based substances such as gasoline, diesel fuel, and fuel oil are monitored for leaks as authorized by the Resource Conservation and Recovery Act (RCRA).
Separation of 20 Amino Acids by Polymer-Based Amino HILIC Column
September 1st 2012Determination and quantification of amino acids are important in many fields. For the HPLC analysis of amino acids, cation exchange mode method has been used with post-column derivatization. Recently, use of reversed-phase mode method is also increasing. However, because of amino acids' hydrophilic nature, they are usually not well retained and have less selectivity by reversed-phase mode without pre-column derivatization steps. In contrast, HILIC mode is an ideal separation mode for hydrophilic compound analysis.
Enhanced Results of Diesel Range Organics Analysis with High-Temperature Gas Enhanced Chromatography
June 1st 2012Diesel range organic (DRO) compounds have boiling points similar to diesel fuel (C10–C28), ranging from 170–430 ?C. These heavy compounds are amenable to GC-FID analysis; however, important considerations should be taken to ensure reproducible results over repeated injections.
The Analysis of 1,4-Dioxane for EPA Method 522 and UCMR 3
June 1st 2012This application note will demonstrate the extraction of 1,4-dioxane from an aqueous matrix using option 1 of EPA Method 522 for 500 mL initial volume sample. It will make use of the SmartPrep Cartridge Extraction system to produce a valid initial demonstration of precision (IDP) and initial demonstration of accuracy (IDA).
Characterization of Polymeric Excipients by SEC-IR
June 1st 2012Excipients are pharmacologically inactive substances used as carrier and release controllers for active drug ingredients. A significant amount (30–80% solid) of polymeric excipients such as hydroxyl propyl cellulose (HPC), hydroxypropyl methyl cellulose (HPMC), hydroxypropyl methyl cellulose acetate succinate (HPMCAS), and povidone are used.
This application demonstrates that stringent sensitivity requirements for the detection of potentially harmful primary aromatic amines can be fulfilled when using an Agilent 1290 Infinity LC system equipped with the 1290 large volume injection kit.
Rapid LC–MS-MS Analysis of Heterocyclic Amines in Salmon
June 1st 2012Heterocyclic amines (HCAs) are a class of compounds that are formed when amino acids from meat proteins react with glucose and creatine under high-temperatures, as is associated with frying or grilling over an open flame (1–3). Studies have correlated the consumption of high levels of HCAs with an increased risk of some forms of cancer.
Detection of Protein Heterogeneity by HPLC
June 1st 2012Protein heterogeneity is generated by post-translational modification, decomposition, and a variety of other processes, including chemical modification, denaturation, and aggregation. Since antibodies and recombinant proteins are now widely used for therapeutic treatment, it is essential to evaluate their heterogeneity during development, stability testing, and in the quality control of the final product.